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Genetic Interactions of Broad Host-Range Plasmid RK2: Evidence for a Complex Replication Regulon

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Plasmids in Bacteria

Abstract

As first recognized by Jacob, Brenner, and Cuzin (13), a successful plasmid must have an efficient replication control system that ensures its stable maintenance by the host. It is now very apparent that bacterial plasmids have evolved an intriguing variety of regulatory strategies to accomplish this (23). However, it is important to realize that for any such system to work effectively, there are certain requirements which concern the plasmid-host interaction. (i) Plasmid genes for replication control must be expressed adequately by the host at the proper time in the cell cycle. (ii) For initiation of replication, the plasmid initiation complex must interact productively with the host’s replication apparatus. (iii) Expression of the plasmid replication determinants must be tightly controlled to prevent an unnecessary metabolic burden or potentially lethal titration of host replication components. (iv) The plasmid must interact with host cell components to guarantee segregation of at least one plasmid copy to each daughter cell at division.

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Author information

Authors and Affiliations

  1. Department of Microbiology and Cancer Center College of Physicians & Surgeons, Columbia University, 701 West 168th Street, 10032, New York, New York, USA

    David H. Figurski, Calvin Young, Helen C. Schreiner & Thomas F. D’ Amico

  2. Department of Biochemistry, University of Minnesota, 55108, Minneapolis, Minnesota, USA

    Robert F. Pohlman

  3. Department of Microbiology, The Public Health Research Institute of the City of New York, 455 First Avenue, New York, New York, 10016, USA

    David H. Bechhofer

  4. Departments of Medicine and Microbiology, College of Physicians and Surgeons, Columbia University, USA

    Alice S. Prince

Authors
  1. David H. Figurski
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  2. Calvin Young
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  3. Helen C. Schreiner
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  4. Robert F. Pohlman
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  5. David H. Bechhofer
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  6. Alice S. Prince
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  7. Thomas F. D’ Amico
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Editor information

Editors and Affiliations

  1. University of California, San Diego, La Jolla, California, USA

    Donald R. Helinski

  2. Stanford University, Stanford, California, USA

    Stanley N. Cohen

  3. University of Michigan, Ann Harbor, Michigan, USA

    Don B. Clewell

  4. Genex Corporation, Gaithersburg, Maryland, USA

    David A. Jackson

  5. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1985 Plenum Press, New York

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Figurski, D.H. et al. (1985). Genetic Interactions of Broad Host-Range Plasmid RK2: Evidence for a Complex Replication Regulon. In: Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., Hollaender, A. (eds) Plasmids in Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2447-8_19

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  • DOI: https://doi.org/10.1007/978-1-4613-2447-8_19

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9487-0

  • Online ISBN: 978-1-4613-2447-8

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