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Role of the π Initiation Protein and Direct Nucleotide Sequence Repeats in the Regulation of Plasmid R6K Replication

  • Chapter
Plasmids in Bacteria

Abstract

Bacterial plasmids vary considerably in size and copy number. A fundamental property shared by these elements, however, is their stable inheritance in a growing population of bacteria. This stable maintenance at a copy number characteristic of the element is determined by genetic regions on the plasmid that regulate the initiation of replication and subsequent partitioning of the plasmid. As in the case of bacterial and animal viruses that employ different mechanisms for their duplication in a particular host cell, plasmids have evolved different strategies to assure their maintenance at a specific copy number. In some cases both primary and secondary control circuits function to regulate the initiation of plasmid replication events and prevent loss of the element in an exponentially growing bacterial population. This paper will be concerned largely with the regulation of replication of the naturally occurring anti-biotic resistance plasmid R6K.

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Author information

Authors and Affiliations

  1. Department of Biology, University of California, San Diego, 92093, La Jolla, California, USA

    Marcin Filutowicz, Michael McEachern, Alan Greener, Pradip Mukhopadhyay, Ross Durland & Donald Helinski

  2. Department of Chemistry, Grinnell College, Grinnell, Iowa, USA

    Elliott Uhlenhopp

Authors
  1. Marcin Filutowicz
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  2. Michael McEachern
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  3. Alan Greener
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  4. Pradip Mukhopadhyay
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  5. Elliott Uhlenhopp
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  6. Ross Durland
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  7. Donald Helinski
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Editor information

Editors and Affiliations

  1. University of California, San Diego, La Jolla, California, USA

    Donald R. Helinski

  2. Stanford University, Stanford, California, USA

    Stanley N. Cohen

  3. University of Michigan, Ann Harbor, Michigan, USA

    Don B. Clewell

  4. Genex Corporation, Gaithersburg, Maryland, USA

    David A. Jackson

  5. Council for Research Planning in Biological Sciences, Inc., Washington, D.C., USA

    Alexander Hollaender

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© 1985 Plenum Press, New York

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Filutowicz, M. et al. (1985). Role of the π Initiation Protein and Direct Nucleotide Sequence Repeats in the Regulation of Plasmid R6K Replication. In: Helinski, D.R., Cohen, S.N., Clewell, D.B., Jackson, D.A., Hollaender, A. (eds) Plasmids in Bacteria. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2447-8_13

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  • DOI: https://doi.org/10.1007/978-1-4613-2447-8_13

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9487-0

  • Online ISBN: 978-1-4613-2447-8

  • eBook Packages: Springer Book Archive

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