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Mutation Induction and Detection in Arabidopsis

  • G. P. Rédei
  • Gregoria N. Acedo
  • S. S. Sandhu
Part of the Environmental Science Research book series (ESRH, volume 31)

Summary

Mutation is an infrequent event, and the stability of the various gene loci is quite different. Additional complexity appears when mutability is studied in different taxonomic groups displaying a variety of genome organization. Higher plants permit the study of mutation in three types of genomes: nuclear, plastidic and mitochondrial. In Arabidopsis the size of the nuclear genome is about 1. 3 × 1012 dalton whereas the chloroplast and the mitochondrial genomes are estimated to be 4 to 5 orders of magnitude smaller. The nuclear genome of Arabidopsis is much less redundant than that of other higher plants, thus mutation can be detected at an estimated 19,688 loci during embryonic development and the total number of loci with visible mutations appears to be 27,875.

Keywords

Mutation Rate Segregation Ratio Mutation Induction Germline Cell Ethyl Methanesulfonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Acedo, G. N. (1983)Laboratory Experiments in Plant TissueCulture, University of Missouri, Columbia, MO.Google Scholar
  2. 2.
    Arntzen, C. J. , K. Pfister, and K. E. Steinack (1982) The mechanism of triazine resistance: Alterations in the cloroplast site of action, InHerbicide Resistance in Plants, La Baron, H. , and J. Gressel, Eds. , Wiley, New York, pp. 185 – 214.Google Scholar
  3. 3.
    Auerbach, D. (1976)Mutation Research, Halsted Press, New York.Google Scholar
  4. 4.
    Broetjes, C. , P. Koene, and J. W. Van Veen (1980) A mutant of a mutant of a mutant of a. . : Radiation-induced mutation in mutation-breeding programme withChrysanthemum morifoliumRam.Euphytica29: 525 – 530.Google Scholar
  5. 5.
    Chapman, R. W. , J. C. Stephens, R. A. Lansman, and J. C. Avise (1982) Models of mitochondrial DNA transmissions genetics and evolution in higher eucaryotes.Genet.Res. , Cambridge 40: 41 – 57.Google Scholar
  6. 6.
    Darr, S. , V. S. Machado, and C. J. Arntzen (1981) Uniparental inheritance of a chloroplast photosystem II polypeptide controlling herbicide binding.Biochim.Biophys.Acta634: 219 – 228.Google Scholar
  7. 7.
    De Greve, H. , J. Leamans, J. P. Harnalsteens, L. Thia-Toong, M. De Beuckeleer, L. Willmitzer, L. Otten, M. Van Montagu, and J. Schell (1982) Regeneration of normal fertile plants that express octopine synthase, from tobacco crown galls after deletion of tumour-controlling functions.Nature300: 752 – 755.Google Scholar
  8. 8.
    de Serres, F. J. , and J. Ashby, Eds. (1981)Evaluation of Short-Term Tests for Carcinogens, Elsevier/North Holland, New York.Google Scholar
  9. 9.
    De Vries, H. (1901)Die Mutationstheorie, Veit, Leipzig, Germany.Google Scholar
  10. 10.
    Galloway, R. E. , and L. Mets (1982) Non-Mendelian inheritance of 3-(3,4-dichlorophenyl)-l, 1-dimethylurea-resistant thyaloid membrane properties inChlamydomonas.Plant Physiol. 70: 1673 – 1677.Google Scholar
  11. 11.
    Gordon, K.H.J., E.J. Crouse, H.J. Bohnert, and R.G. Herrmann (1982) Physical mapping of the differences in chloroplast DNA of five wild-type plastomes in Oenothera subsection Euoenothera. Theor. Appl. Genet. 61:373–381Google Scholar
  12. 12.
    Howell, S.H. (1982) Plant molecular vehicles: Potential vectors for introducing foreign DNA into plants. Annu. Rev. Plant Phys. 33:609–650 Google Scholar
  13. 13.
    Kamalay, J. C. , and R. B. Goldberg (1980) Regulation of structural gene expression in tobacco.Cell19: 935 – 946.Google Scholar
  14. 14.
    Kastenbaum, M.A., and K.O. Bowman (1966) The minimum significant number of successes in a binomial sample. Oak Ridge Natl. Lab. QRNL-3909, pp. 1–69. Google Scholar
  15. 15.
    Kiper, M. , D. Bartels, and H. Kochel (1979) Gene number estimates in plant tissues and cells, InPlant Systernatics and Evolution, Genome and Chromatin: Organization, Evolution,Function, Naglf W. , V. Hemleben, and F. Ehrendorfer, Eds. , Springer, New York, pp. 129 – 140.Google Scholar
  16. 16.
    Kung, S. D. , Y. S. Zhu, and G. F. Shen (1982)Nicotianachloroplast genome III. Chloroplast DNA evolution.Theor. Appl. Genet. 61:73–79.Google Scholar
  17. 17.
    Lewin, R. (1983) Promiscuous DNA leaps all barriers.Science219:478–479. Google Scholar
  18. 18.
    Li, S. L. , and G. P. Redei (1969a) Thiamine mutants of the cruciferArabidopsis.Biochem.Genet. 3: 163 – 170.Google Scholar
  19. 19.
    Li, S. L. , and G. P. Redei (1969b) Estimation of nutation rates in autogamous diploids.Radiat.Bot. 9: 125 – 131.Google Scholar
  20. 20.
    Luria, S. E. , and M. Delbruck (1943) Mutations of bacteria from virus sensitivity to virus resistance.Genetics28: 491 – 511.Google Scholar
  21. 21.
    Marx, J. L. (1983) A transposable element of maize emerges.Science219: 829 – 830.Google Scholar
  22. 22.
    Maugh, T. H. , II (1978) Chemicals: How many are there?Science199: 162.ADSGoogle Scholar
  23. 23.
    Michaelis, P. (1955) Ober Gesetmassigkeiten der Plasmon-Unikombination und uber eine Methode zur Trennung einer Plastiden-, Chondriosomen-, resp. , Sphaerosomen- (Mikrosomen)- und einer Zytoplasma-Vererbung.Cytologia20: 315 – 338.Google Scholar
  24. 24.
    Micke, A. (1975) Induced mutations in plant breeding.Can.J.PlantSci. 55: 865.Google Scholar
  25. 25.
    Muller, A. J. (1963) Embryonentest zum Nachweis recessiver Latalfaktoren beiArabidopsis thaliana.Biol.Zbl. 83: 133 – 163.Google Scholar
  26. 26.
    Orihara, Y. , and K. Tsunewaki (1982) Molecular basis of the genetic diversity of the cytoplasm inTriticumandAegilopsI. Diversity of the chloroplast and its lineage revealed by the restriction pattern of ct-DNAs.Japan J.Genet. 57: 371 – 396.Google Scholar
  27. 27.
    Redei, G. P. (1965) Genetic blocks in the thiamine synthesis of the angiospermArabidopsis.Amer.J.Bot. 52: 834 – 841.Google Scholar
  28. 28.
    Redei, G. P. (1970)Arabidopsis thaliana(L. ) Heynh. A review of the genetics and biology.Bibliogr.Genet. 20:1–151.Google Scholar
  29. 29.
    Redei, G. P. (1973) Extrachromosomal mutability determined by a nuclear gene locus inArabidopsis.Mutation Res. 18: 149 – 162.Google Scholar
  30. 30.
    Redei, G. P. (1975)Arabidopsisas a genetic tool.Annual Rev.Genet. 9:111–127.Google Scholar
  31. 31.
    Redei, G. P. (1982)Genetics, Macmillan, New York.Google Scholar
  32. 32.
    Redei, G. P. , G. N. Acedo, and S. S. Sandhu (1983) Sensitivity, specificity and accuracy of theArabidopsisassay in the identification of carcinogens, (in this volume, pp. 691–710).Google Scholar
  33. 33.
    Redei, G. P. , and S. L. Li (1969b) Effects of X-rays and ethyl methanesulfonate on the chlorophyll b locus in the soma and on the thiamine loci in the germline ofArabidopsis.Genetics61: 453 – 459.Google Scholar
  34. 34.
    Rédei, G. P. , and S. B. Plurad (1973) Hereditary structural alterations of plastids induced by a nuclear mutator gene inArabidopsis.Protoplasma77: 361 – 380.Google Scholar
  35. 35.
    Robbelen, G. (1962) Plastomemutationen nach Rontgen-bestrahlung vonArabidopsis thaliana(L. )Heynh.Zeitschr.Vererb. -Lehre93: 25 – 34.Google Scholar
  36. 36.
    Robbelen, G. (1966) Chloroplastendifferenzierung nach geninduzierter Plastommutation beiArabidopsis thaliana(L. )Heynh.Z.Pflanzenphysiol. 55: 387 – 403.Google Scholar
  37. 37.
    Solignac, M. , and J. Genermont (1982) Les estimations du nombre de genes et la complexification des genomes.Ann.Biol. 21 (3): 210 – 273.Google Scholar
  38. 38.
    Spradling, A. C. , and G. M. Rubin (1982) Transposition of cloned P elements intoDrosophilagerm line chromosomes.Science218: 341 – 347.Google Scholar
  39. 39.
    Stadlerf L. J. (1928) Mutations in barley induced by X-rays and radium.Science68: 186 – 187.Google Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • G. P. Rédei
    • 1
  • Gregoria N. Acedo
    • 1
  • S. S. Sandhu
    • 2
  1. 1.Department of AgronomyUniversity of MissouriColumbiaUSA
  2. 2.Environmental Protection AgencyUSA

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