Abstract
The development of analytical methods for HPLC has in the past been based on experience, intuition and serendipidity. With the high efficiency of present-day columns, the main scope for improving separations lies in adjusting the mobile phase for selectivity. Optimization has been fostered (Snyder, Kirkland) by a ‘Selectivity Triangle’ based on solution interactions — acidic, basic and dipole. Thereby optimal resolution of components for a particular column is achievable by choosing solvents from a relatively small range.
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References
Snyder, L.R. (1978) J. Chromatog. Sci. 16, 223 – 234
Snyder, L.R. (1980) in High Performance Liquid Chromatography Vol. 1 (Horvath, C., ed.), Academic Press, New York, pp. 207 – 331
Snyder, L.R., Dolan, J.W. & Grant, J.R. (1979) J. Chromatog. 165, 3 – 30.
Glajch, J.L., Kirkland, J.J., Squire, K.M. & Minor, J.M. (1980) J. Chromatog. 199, 57 – 79
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© 1984 Plenum Press, New York
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Cox, G.B. (1984). Multi-Solvent Optimization of HPLC Separations. In: Reid, E., Wilson, I.D. (eds) Drug Determination in Therapeutic and Forensic Contexts. Methodological Surveys in Biochemistry and Analysis, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2397-6_9
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DOI: https://doi.org/10.1007/978-1-4613-2397-6_9
Publisher Name: Springer, Boston, MA
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