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Development and Application of an HPLC Pre-Concentration Device Suitable for Large Amounts of Biological Sample

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Drug Determination in Therapeutic and Forensic Contexts

Part of the book series: Methodological Surveys in Biochemistry and Analysis ((ANAY,volume 14))

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Abstract

A cone-shaped HPLC pre-column has been developed to cope with the problems that arise during the work-up of large volumes of biological sample. The device offers large loading capacity at the front of the pre-column. It is constructed of stainless steel and PCTFE. Its pressure resistance (up to 40 MPa) allows full integration into HPLC systems. It is observed that there is scarcely any decrease in performance on going from a small to a large sample volume using the cone-shaped pre-column. Its possibilities in biomedical research are discussed, with metabolic examples.

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References

  1. Roth, W., Beschke, K., Jauch, R., Zimmer, A. & Koss, F.W. (1980) J. Chromatog. 222, 13 – 22.

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Author information

Authors and Affiliations

  1. Duphar Research Laboratories, P.O. Box 2, Weesp, The Netherlands

    H. M. Ruijten, P. H. van Amsterdam & H. de Bree

Authors
  1. H. M. Ruijten
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  2. P. H. van Amsterdam
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  3. H. de Bree
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Editor information

Editors and Affiliations

  1. Guildford Academic Associates, Guildford, Surrey, UK

    Eric Reid

  2. Hoechst Pharmaceutical Research Laboratories, Milton Keynes, UK

    Ian D. Wilson

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© 1984 Plenum Press, New York

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Ruijten, H.M., van Amsterdam, P.H., de Bree, H. (1984). Development and Application of an HPLC Pre-Concentration Device Suitable for Large Amounts of Biological Sample. In: Reid, E., Wilson, I.D. (eds) Drug Determination in Therapeutic and Forensic Contexts. Methodological Surveys in Biochemistry and Analysis, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2397-6_8

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  • DOI: https://doi.org/10.1007/978-1-4613-2397-6_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9462-7

  • Online ISBN: 978-1-4613-2397-6

  • eBook Packages: Springer Book Archive

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