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Simplified Approaches to the HPLC Determination of Drugs in Biological Samples

  • G. S. Clarke
  • L. K. Liu
  • M. L. Robinson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 14)

Abstract

The simplest approach to sample preparation for HPLC is centrifugation and/or filtration of the sample, followed by direct injection onto the column. This is particularly suitable for drugs which are not bound to the matrix, and where concentrations are well above the limit of detection. An example of this approach is the analysis of cephradine in urine (1–100 µg/ml), as reported from this laboratory in an earlier volume of this series [l]. Another example of direct injection (sometimes with pre-dilution) is a reported assay for cefmenoxime in urine [2].

Keywords

Analytical Column Short Column HPLC Determination Early Volume Senior Editor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Forster, T.C., Heyes, W.F., Jones, G.T. & Wood, P.R. (1978) in Blood Drugs and Other Analytical Challenges (Reid, E., ed.) [Vol. 7, this series], Horwood, Chichester, pp. 341 – 344.Google Scholar
  2. 2.
    Noonan, I.A., Gambertoglio, J.G., Barriere, S.L., Conte, J.E. & Lin, E.T. (1983) J. Chromatog. 273, 458 – 463CrossRefGoogle Scholar
  3. 3.
    Burns, D.A. (1981) Pharm. Tech. 5, 53 – 60.Google Scholar
  4. 4.
    Koch, D.D. & Kissinger, P.T. (1980) Anal. Chem. 52, 27 – 29CrossRefGoogle Scholar
  5. 5.
    Ascalone, V. & Dal Bo, L. (1983) J. Chromatog. 273, 357 – 366.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • G. S. Clarke
    • 1
  • L. K. Liu
    • 1
  • M. L. Robinson
    • 1
  1. 1.E.R. Squibb & SonsInternational Development LaboratoriesMoreton, MerseysideUK

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