Advent and Present-Day Status of Ligand Methods
Biological methods have been crucial in pharmacological studies since the earliest days of quantitation. Disciplined use of bioassay allowed the discovery and quantitation of such endogenous compounds as acetylcholine and noradrenaline long before chemical assay became possible. In contrast, little was done in attempts to measure exogenous compounds introduced into the body until key developments in chemical analysis took place, notably with the Bratton-Mar shall reaction, absorption and fluorescence spectroscopy and, more recently, GC and HPLC. Specificity in chemical drug assays became a rigorous aim, often hinging on both work-up procedures and detection modes.
KeywordsMorphine Cocaine Methotrexate NADPH Charcoal
Unable to display preview. Download preview PDF.
- 1.Maggio, E.T., ed. (1980) Enzyme Immunoassay, CRC Press, Boca Raton, Florida, 295 pp.Google Scholar
- 2.Burgen, A.S.V. & Mitchell, J.F. (1978) Gaddum’s Pharmacology, 8th edn., Oxford Univ. Press, Oxford. - See pp. 229 – 247.Google Scholar
- 3.Alcid, D.V. & Seligman, S.J. (1973) Antimicrobial Agents & Chemotherapy 3, 559 – 561.Google Scholar
- 4.Berther, A. & Redfors, A. (1970) Clin. Pharmacol. Ther. 11, 665 – 673.Google Scholar
- 6.Schall, R.F. & Teroso, H.J. (1981) Clin. Chem. 27, 1157 – 1164.Google Scholar
- 7.Wisdom, G.B. (1976) Clin. Chem. 22, 1243 – 1255.Google Scholar
- 8.Falk, L.C., Clard, D.R., Kalman, S.M. & Long, T.F. (1976) Clin. Chem. 22, 785 – 788.Google Scholar
- 11.Burd, J.F., Wong, R.C., Feenery, J.E., Carrico, R.J. & Bogulaski, R.C. (1977) Clin. Chem. 23, 1402 – 1408.Google Scholar
- 13.Curry, S.H. (1970) Br. J. Pharmacol. 39, 250 P.Google Scholar