Abstract
Tamoxifen is an anti-oestrogenic agent widely used in the treatment of breast cancer. Dose levels are typically 20 mg/day, sometimes after an initial loading dose of 40 mg. Two main metabolites are observed, N-desmethyltamoxifen and 4-hydroxytamoxifen. (For formulae, see #D-2.) The stated dosage gives tamoxifen and N-desmethyltamoxifen levels of 150-250 ng/ml plasma, and 4-hydroxytamoxifen levels of <8 ng/ml [l]. This Note describes an HPLC method for the determination of tamoxifen and desmethyltamoxifen down to 25 ng/ml plasma. 4-Hydroxytamoxifen would need a more sensitive method (cf.#D-4).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Golander, Y. & Sternson, L.A. (1980) J. Chromatog. 181
Stevenson, D & Reid, E. (1981) Anal. Lett. 14B
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Plenum Press, New York
About this chapter
Cite this chapter
Stevenson, D. (1984). Determination of Tamoxifen and N-Desmethyltamoxifen in Plasma by HPLC. In: Reid, E., Wilson, I.D. (eds) Drug Determination in Therapeutic and Forensic Contexts. Methodological Surveys in Biochemistry and Analysis, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2397-6_30
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2397-6_30
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9462-7
Online ISBN: 978-1-4613-2397-6
eBook Packages: Springer Book Archive