HPLC Analysis of Cisplatin Analogues in Biological Fluids

  • D. R. Newell
  • Z. H. Siddik
  • K. R. Harrap
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 14)

Abstract

The cisplatin analogues investigated relate to the search for a second generation Pt complex less toxic or more active than the parent compound. For cis-diammine(1,1-cyclobutane-dicarboxylato) platinum (CBDCA), normal-phase chromatography on silica with UV detection suffices down to levels of 10 μM in urine or plasma ultrafiltrates. Quantitation below 10 μM needs HPLC fraction collection followed by flameless atomic absorption spectrophotometry (FAAS; 0.5 μM detectable). These methods have been successfully applied to CBDCA in plasma and urine from patients and rats [1, 2]. For cis-dichloro- trans -dihydroxy bis (isopropylamine) platinum (CHIP), plasma ultrafiltrates were analyzed by HPLC on a μBondapak phenyl column with UV detection [3], or by water /methanol gradient elution from a μBondapak C-18 column followed by fraction collection and FAAS [4] (detection limits 1 μM and 0.04 μM respectively). Urines containing CHIP were analyzed by the latter method [3, 5]. These assays have been successfully applied to dog and human samples where besides CHIP, cis-dichloro-bis(isopropylamine)platinum has been found [3]. [Formulae are shown overleaf.-Ed.]

Keywords

HPLC Platinum Acetonitrile MeOH Cyclohexane 

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Copyright information

© Plenum Press, New York 1984

Authors and Affiliations

  • D. R. Newell
    • 1
  • Z. H. Siddik
    • 1
  • K. R. Harrap
    • 1
  1. 1.Department of Biochemical PharmacologyInstitute of Cancer ResearchSuttonSurrey, UK

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