Skip to main content
SpringerLink
Log in

Relationship between Calcium, Arachidonic Acid Metabolites, and Neutrophil Activation

  • Chapter
Calcium in Biological Systems

Abstract

The neutrophils represent the first line of defense against foreign and pathogenic elements, and in so doing must perform several functions. In response to chemotactic factors, they detect, move toward, and accumulate at the site of infection. This phenomenon is termed chemotaxis. Once at the site of infection, they ingest or phagocytize the appropriate particles. The phagocytic process is followed by the discharge of the neutrophils’ various lysosomes and granules into the phagocytic vacuole thereby promoting degradation and digestion of the engulfed particle or organism. Thus, most neutrophil functions such as motility, cell shape change, phagocytosis, and degranulation that are activated by chemotactic factors depend on the mechanical displacement of part of, or all of, the cell. Mechanochemical coupling is thus an essential component of neutrophil responsiveness. In addition to eliciting various neutrophil functions, chemotactic factors activate several biochemical events, including stimulation of certain membrane-bound enzymes, oxidative metabolism, and actin associated with the cytoskeleton [4,22,30,31,33].

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Abdel-Latif, A. A.; Luke, B.; Smith, J. P. Studies on the properties of the soluble phosphatidylinositol- phosphodiesterase of rabbit iris smooth muscle. Biochim. Biophys. Acta 614: 425–434, 1980.

    PubMed  CAS  Google Scholar 

  2. Akhtar, R. A.; Abdel-Latif, A. A. Studies on the properties of triphosphoinositide phosphodiesterase and phosphodiesterase of rabbit iris smooth muscle. Biochim. Biophys. Acta 527: 150–170, 1978.

    Google Scholar 

  3. Bareis, D. L.; Hirata, F.; Schiftmann, E.; Axelrod, J. Phospholipid metabolism, calcium flux and the receptor- mediated induction of chemotaxis in rabbit neutrophils. J. Cell Biol 93: 690–697, 1982.

    Article  PubMed  CAS  Google Scholar 

  4. Becker, E. L.; Naccache, P. H.; Showell, H. J.; Walenga, R. W. Early events in neutrophil activation: Receptor stimulation, ionic fluxes and arachidonic acid metabolism. In: Lymphokines, Volume 4, E. Pick and M. Land, eds., New York, Academic Press, 1981, pp. 194–334.

    Google Scholar 

  5. Berridge, N. J. A novel cellular signaling system based on the integration of phospholipid and calcium metabolism. In: Calcium and Cell Function, Volume 3, W. Y. Cheung, ed., New York, Academic Press, 1983, pp. 1–36.

    Google Scholar 

  6. Billah, M. M.; Lapetina, E. G. Rapid decrease of phosphatidylinositol 4,5-bisphosphate in thrombin-stimulated platelets. J. Biol. Chem 257: 12705–12708, 1982.

    PubMed  CAS  Google Scholar 

  7. Bokoch, G.; Reed, P. W. Stimulation of arachidonic metabolism in the polymorphonuclear leukocyte by an N- formylated peptide. J. Biol. Chem 255: 10223–10226, 1980.

    PubMed  CAS  Google Scholar 

  8. Cockcroft, S.; Bennett, J. P.; Gomperts, B. D. Stimulus-secretion coupling in rabbit neutrophils is not mediated by phosphatidylinositol breakdown. Nature (London) 288: 275–277, 1980.

    Article  CAS  Google Scholar 

  9. Feinstein, M. B.; Sha’afi, R. I. The role of calcium in arachidonic acid metabolism and in the actions of arachidonic acid-derived metabolites. In: Calcium and Cell Function, Volume 4, W. Y. Cheung, ed., New York, Academic Press, 1983, pp. 337–376.

    Google Scholar 

  10. Garcia-Castro, I.; Mato, J. M.; Vasanthakumas, G.; Wiesmann, W. P.; Schiffmann, E.; Chiang, P. K. Paradoxical effects of adenosine on neutrophil chemotaxis. J. Biol. Chem 258: 4345–4349, 1983.

    PubMed  CAS  Google Scholar 

  11. Goetzl, E. J.; Foster, D. W.; Goldman, D. W. Specific effects on human neutrophils of antibodies to a membrane protein constituent of neutrophil receptors for chemotactic formyl-methionyl peptides. Immunology 45: 1–8, 1982.

    Google Scholar 

  12. Griffin, H. D.; Hawthorne, J. N. Calcium-activated hydrolysis of phosphatidyl-myo-inositol 4-phosphate and phosphatidyl-myo-inositol 4,5-bis phosphate in guinea pig synaptosomes. Biochem. J 176: 541–552, 1978.

    PubMed  CAS  Google Scholar 

  13. Hirata, F.; Corcoran, B. A.; Venkatasubramanian, K.; Schiffmann, E.; Axelrod, J. Chemoattractants stimulate degradation of methylated phospholipids and release of arachidonic acid in rabbit leukocytes. Proc. Natl. Acad. Sci. USA 76: 2640–2643, 1979.

    Article  PubMed  CAS  Google Scholar 

  14. Hotchkiss, A.; Jordan, J. V.; Hirata, F.; Schulman, N. R.; Axelrod, J. Phospholipid methylation and human platelet function. Biochem. Pharmacol 30: 2089–2095, 1981.

    Article  PubMed  CAS  Google Scholar 

  15. Jackowski, S.; Sha’afi, R. I. Response of adenosine cyclic 3′,5′-monophosphate levels in rabbit neutrophils to the chemotactic peptide formyl-methionyl-phenylalanine. Mol. Pharmacol 16: 473–481, 1979.

    PubMed  CAS  Google Scholar 

  16. Jubiz, W.; Radmark, O.; Malmsten, C.; Hansson, G.; Lindgrent, J. A.; Palmblad, J.; Uden, A.-M.; Sam- uelsson, B. A novel leukotriene produced by stimulation of leukocytes with formylmethionylleucylphenylala- nine. J. Biol. Chem 257: 6106–6110, 1982.

    PubMed  CAS  Google Scholar 

  17. Korchak, H. M.; Weissmann, G. Stimulus-response coupling in the human neutrophil: Transmembrane potential and the role of extracellular Na+. Biochim. Biophys. Acta 601: 180–194, 1980.

    Article  PubMed  CAS  Google Scholar 

  18. Michell, R. H.; Kirk, C. J. The unknown meaning of receptor-stimulated inositol lipid metabolism. Trends. Pharmacol. Sci 3: 1490–1491, 1982.

    Google Scholar 

  19. Niedel, J. E.; Cuatrecasas, P. Formyl peptide chemotactic receptors of leukocytes and macrophages. Curr. Top. Cell. Regul 27: 137–170, 1982.

    Google Scholar 

  20. Rubin, R. P.; Sink, L. E.; Freer, R. J. On the relationship between formyl methionyl-leucyl-phenylalanine stimulation of arachidonyl phosphatidylinositol turnover and lysosomal enzyme secretion by rabbit neutrophils. Mol. Pharmacol 19: 31–73, 1981.

    PubMed  CAS  Google Scholar 

  21. Seligmann, B. D.; Gallin, J. L. Use of lipophilic probes of membrane potential to assess human neutrophil activation: Abnormality in chronic granulomatous disease. J. Clin. Invest 66: 493–503, 1980.

    Article  PubMed  CAS  Google Scholar 

  22. Sha’afi, R. I.; Naccache, P. H. Ionic movements in neutrophil chemotaxis and secretion. In: Advances in Inflammation Research, Volume 2, G. Weissmann, ed., New York, Raven Press, 1981, pp. 115–148.

    Google Scholar 

  23. Sha’afi, R. I.; Naccache, P. H.; Molski, T. F. P.; Volpi, M. Chemotactic stimuli-induced changes in the pH of rabbit neutrophils. In: Intracellular pH: Its Measurements, Regulation and Utilization in Cellular Functions, Kroc Foundation Series, Volume 15, R. Nuccitelli and D. W. Deamer, New York, Liss, 1982, pp. 513–525.

    Google Scholar 

  24. Showell, H. J.; Naccache, P. H.; Sha’afi, R. I.; Becker, E. L. The effects of extracellular K+, Na+, and Ca2+ on lysosomal enzyme secretion from polymorphonuclear leukocytes. J. Immunol 119: 804–811, 1977.

    PubMed  CAS  Google Scholar 

  25. Simchowitz, L.; Fischbein, L. L.; Spilberg, I.; Atkinson, J. P. Induction of a transient elevation in intracellular levels of adenosine-3′,5′-cyclic monophosphate by chemotactic factors: An early event in human neutrophil activation. J. Immunol 124: 1482–1491, 1980.

    PubMed  CAS  Google Scholar 

  26. Simchowitz, L.; Spilberg, I.; Atkinson, J. P. Evidence that functional responses of human neutrophils occur independently of transient elevations in cAMP levels. 67th Annual Meeting of FASEB 42: 1080, 1983.

    Google Scholar 

  27. Tsien, R. Y.; Pozzan, T.; Rink, T. J. Calcium homeostasis in intact lymphocytes: Cytoplasmic free calcium monitored with a new, intracellular trapped fluorescent indicator. J. Cell Biol 94: 325–334, 1982.

    Article  PubMed  CAS  Google Scholar 

  28. Volpi, M.; Naccache, P. H.; Sha’afi, R. I. Calcium transport in inside-out membrane vesicles prepared from rabbit neutrophils. J. Biol. Chem 258: 4153–4158, 1983.

    PubMed  CAS  Google Scholar 

  29. Volpi, M.; Yassin, R.; Naccache, P. H.; Sha’afi, R. I. Chemotactic factor causes rapid decreases in phos-phatidylinositol 4,5 bis-phosphate and phosphatidylinositol 4-monophosphate in rabbit neutrophils. Biochem. Biophys. Res. Commun 112: 957–964, 1983.

    Article  PubMed  CAS  Google Scholar 

  30. Weissmann, G.; Smolen, J.; Korchak, H.; Hoffstein, S. The secretory code of the neutrophils. In: Cellular Interactions, J. T. Dingle and J. L. Gordon, eds., Amsterdam, Elsevier/North-Holland, 1980, pp. 15–31.

    Google Scholar 

  31. White, J. R.; Naccache, P. H.; Sha’afi, R. I. The synthetic chemotactic peptide formyl-methionyl-leucyl- phenylalanine causes an increase in actin associated with the cytoskeleton in rabbit neutrophils. Biochem. Biophys. Res. Commun 108: 1144–1149, 1982.

    Article  PubMed  CAS  Google Scholar 

  32. White, J. R.; Naccache, P. H.; Molski, T. F. P.; Borgeat, P.; Sha’afi, R. I. Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factors. Biochem. Biophys. Res. Commun 113: 44–50, 1983.

    Article  PubMed  CAS  Google Scholar 

  33. Wilkinson, D. C. Chemotaxis and Inflammation, 2nd ed., Edinburgh, Churchill Livingstone, 1982.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut, 06032, USA

    Ramadan L. Sha’afi

  2. Department of Pathology, University of Connecticut Health Center, Farmington, Connecticut, 06032, USA

    Paul H. Naccache

Authors
  1. Ramadan L. Sha’afi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paul H. Naccache
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia, USA

    Ronald P. Rubin  & James W. Putney Jr.  & 

  2. CIBA-GEIGY Corporation, Summit, New Jersey, USA

    George B. Weiss

Rights and permissions

Reprints and permissions

Copyright information

© 1985 Plenum Press, New York

About this chapter

Cite this chapter

Sha’afi, R.L., Naccache, P.H. (1985). Relationship between Calcium, Arachidonic Acid Metabolites, and Neutrophil Activation. In: Rubin, R.P., Weiss, G.B., Putney, J.W. (eds) Calcium in Biological Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2377-8_16

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2377-8_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9453-5

  • Online ISBN: 978-1-4613-2377-8

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation