Abstract
The outer membrane of gram-negative bacteria has proven to be a valuable model system in which to study the many complexities of biomembrane structure, function, and biogenesis which are common not only to prokaryotic but also eukaryotic cells. The advantages in utilizing the Escherichia coli outer membrane to examine the various aspects of membrane biology are (1) the outer membrane consists of relatively few major proteins, many of which have been purified, (2) a finely detailed genetic map for E. coli as well as the structure of the genes coding for many of these proteins have been established, and (3) all of the outer membrane proteins have been shown to be synthesized in the cytoplasmic compartment and subsequently secreted across the cytoplasmic membrane prior to assembly in the outer membrane. Therefore, by utilizing these aspects of the E. coli outer membrane, one can directly address many of the fundamental questions concerning biomembranes including the functions and structure of integral membrane proteins, molecular mechanisms of biosynthesis and assembly of membrane proteins, interactions between membrane proteins themselves and with other membrane components, regulation of membrane biogenesis, and mechanisms of protein translocation across bilayer membranes.
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Vlasuk, G.P., Ghrayeb, J., Inouye, M. (1985). The Major Outer Membrane Lipoprotein of Escherichia coli:Secretion, Modification, and Processing. In: Martonosi, A.N. (eds) The Enzymes of Biological Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2355-6_9
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DOI: https://doi.org/10.1007/978-1-4613-2355-6_9
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