Abstract
All 22 enzymic steps in the synthesis of cholesterol (Figure 1) from squalene are catalyzed by membrane-bound enzymes of the endoplasmic reticulum which is isolated as the microsomal fraction of cell-free tissue preparations. In addition, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA reductase), the rate-limiting enzyme for the overall cholesterol biosynthetic pathway, is also membrane-bound and found associated with microsomes. These observations are significant since they suggest that the membrane may play important roles in both the catalysis and regulation of synthesis of cellular sterols.
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Trzaskos, J.M., Gaylor, J.L. (1985). Membrane-Bound Enzymes of Cholesterol Biosynthesis:Resolution and Identification of the Components Required for Cholesterol Synthesis from Squalene. In: Martonosi, A.N. (eds) The Enzymes of Biological Membranes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2355-6_5
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