Practical Aspects of ICE-free Cryopreservation
Low temperatures are used by biologists primarily because they slow or prevent unwanted physical and chemical events. Unfortunately, the utility of low temperatures is usually compromised by the inconvenient fact that cooling also leads to the crystallization of water and thereby creates new and unwanted physical and even chemical events which may injure the system the biologist wishes to preserve. Although the penalties imposed by freezing are in many cases acceptable, ice formation renders biological preservation generally imperfect and sometimes inconvenient.
KeywordsCrystallization Toxicity Glycerol Bromide Propylene
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