The Importance of Matrix Interactions and Tissue Topography for the Growth and Differentiation of Mammary Epithelial Cells in Vitro
Mammary gland morphogenesis and mammary epithelial cell proliferation and functional differentiation are processes which normally require various interactions, such as those between different cells, between cells and extracellular matrix components, and between cells, hormones, and growth factors. How such interactions are coupled in vivo to bring about controlled mammary cell growth and normal cell function remains to be established; clearly, it is a problem that cannot be easily addressed experimentally in the animal. The last few years have seen considerable progress in the development of culture systems which permit an examination in vitro of such interactions crucial either to normal cell behaviour or to transformed cell characteristics. Thus, it is now possible to dissociate mammary cells from the normal topological relationships that exist in vivo and provide them with an in vitro environment which permits growth and reorganization of individual cells and small units into structures with the proper tissue topography which can express markers of epithelial cell function in response to hormonal stimuli.
KeywordsMigration Filtration Lactate Hydrocortisone Polypeptide
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