Abstract
Most of our knowledge on the subcellular events taking place from the moment that the action potential invades the nerve terminal until response is recorded in the postsynaptic cell comes from experiments performed on the neuromuscular synapse. This preparation which is most suitable for electrophysiological studies is unfortunately not adequate for biochemical experiments. Therefore, we are using synaptosomes from brain (intact or following osmotic disruption) as the alternative biochemical model for studying calcium transport systems in nerve terminals. Synaptosomes are the closest biochemical model structures to intact synapses (1, 2). They maintain membrane potentials as measured by voltage sensitive fluorescent probes (3), have osmotic properties similar to an intact cell, are more permeable to K than to Na (1) and release neurotransmitters in media containing calcium — provided depolarizing potassium concentrations or other depolarizing agents are present (1, 2, 3).
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© 1986 Martinus Nijhoff Publishing, Boston
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Rahamimoff, H., Barzilai, A., Erdreich, A., Spanier, R. (1986). Molecular Properties of Isolated Ca2+ Transport Systems from Nerve Terminals. In: Rahamimoff, R., Katz, B. (eds) Calcium, Neuronal Function and Transmitter Release. Topics in the Neurosciences, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2307-5_5
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DOI: https://doi.org/10.1007/978-1-4613-2307-5_5
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