Abstract
Katz (1) in his Sherrington lecture summarized our understanding of the microphysiology of the presynaptic terminals of the vertebrate neuromuscular junction (nmj). The arrival of a nervous impulse causes a transient increase in the calcium permeability of the plasma membrane and the associated entry of calcium ions triggers the synchronized release of quanta of transmitter. The magnitude of the evoked response, recorded as the endplate potential (EPP) is therefore primarily dependent on the concentration of extracellular calcium (2). There is also a spontaneous release of quantal packets of acetylcholine (ACh), recorded as the miniature endplate potentials (MEPPs), and the MEPP frequency is determined, at least in part, on the other hand, by the concentration of intracellular calcium at the terminals (3, 4). However, we still lack a firm conclusion as to how the intracellular calciumions trigger both the spontaneous and the evoked release of transmitter. Since the earlier biophysical studies of Katz and Miledl a large number of pharmacological agents have been tested on the vertebrate nmj, and the purpose of this very brief review is to attempt to determine their sites of action in the hope of discovering some of the biochemical events of exocytosis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Katz B: The release of neural transmitter substances. Liverpool University Press, 1969.
Crawford AC: The dependence of evoked transmitter release on external calcium ions at very low mean quantal contents. J. Physiol. 240: 255–278, 1974.
Alnaes E, Rahamimoff R: On the role of mitochondria in transmitter release from motor nerve terminals. J. Physiol. 248: 285–306. 1975.
Statham HE, Duncan CJ: The action of ionophores at the frog neuromuscular junction. Life Sci. 17: 1401–1406, 1975.
Thesleff S, Molgo J: A new type of transmitter release at the neuromuscular junction. Neuroscience 9: 1–8, 1983.
Tauc, L: Nonvesicular release of neurotransmitter. Physiol Rev. 62: 857–893, 1982.
Blaustein MP, Ratzlaff RW, Kendrick NK: The regulation of intracellular calcium in presynaptic nerve terminals. Ann. N.Y. Acad. Sci. 307: 195–212, 1978.
Blaustein MP, Ratzlaff RW, Schweitzer ES: Control of intracellular calcium in presynaptic nerve terminals. Federation Proc. 39: 2790–2795, 1980.
Glossmann H: Molecular approach to the calcium channel. XI Congress International Society for Heart Research, London 1983.
Ferry DR, Goll A, Glossmann H: Putative calcium channel molecular weight determination by target size analysis. N.S. Arch. Pharmacol. 323: 292–297, 1983.
Nicholls DG, Åkerman KEO: Biochemical approaches to the study of cytosolic calcium regulation in nerve endings. Phil. Trans. Roy. Soc. Lond. 296: 115–122, 1981.
Duncan CJ: Properties of the Ca2+-ATPase activity of mammalian synaptic membrane preparations. J. Neurochem. 27: 1277–1279, 1976.
Akerman KEO, Nicholls DG: Ca2+ transport and the regulation of transmitter release in isolated nerve endings. Trends in Biochem. Sci. 8: 63–4, 1983.
Lin SC, Way EL: Calcium-activated ATPases in presynaptic nerve endings. J. Neurochem. 39: 1641–1651, 1982.
Ross DH, Cardenas HL: Calmodulin stimulation of Ca2+-dependent ATP hydrolysis and ATP-dependent Ca2+ transport in synaptio membranes. J. Neurochem. 41: 161–171, 1983.
Campbell AJ: Intracelular calcium. Its universial role as regulator. John Wiley & Sons Ltd, 1983.
Blaustein MP, Ratzlaff RW, Kendrick NC, Schweitzer, ES: Calcium buffering in presynaptic nerve terminals. I. Evidence for involvement of a nonmitochondrial ATP-dependent sequestration mechanism. J. Gen. Physiol. 72: 15–41, 1978.
Tillotson DL, Gorman ALF: Localization of neuronal Ca2+ buffering near plasma membrane studied with different divalent cations. Cell. Molec. Neurobiol. 3: 297–310, 1983.
Lüllmann, H, Peters T, Preuner J: Role of the plasmalemma for calcium homeostasis and for excitation-contraction coupling in cardiac muscle. In: AJ Drake-Holland and MIM Noble (eds) Cardiac Metabolism. John Wiley & Sons Ltd, 1983, p. 1.
Blaustein MP, Ratzlaff RW, Schweitzer, ES: Calcium buffering in presynaptic nerve terminals. II. Kinetic properties of the nonmitochondrial Ca sequestration mechanism. J. Gen. Physiol. 72: 43–66, 1978.
Somlyo AP: Cellular site of calcium regulation. Nature 309: 516–517, 1984.
Molgo J, Thesleff S: Studies on the mode of action of botulinum toxin type A at the frog neuromuscular junction. Brain Res. 297: 309–316, 1984.
Rahamimoff H, Abramovitz E: Ca transport and ATPase activity of synaptosomal vesicles from rat brain. FEBS Letts. 92: 163–167, 1978.
Israel M, Manaranche R, Marsal J, Meunier FM, Morel N, Frachon P, Lesbats B: ATP-dependent calcium uptake by cholinergic synaptic vesicles isolated from Torpedo electric organ. J. Membr. Biol. 54: 115–126, 1980.
Rephaeli A, Parsons SM: Calmodulin stimulation of 45Ca2+ transport and protein phosphorylation in cholinergic synaptic vesicles. Proc. Nat. Acad. Sci. U.S.A. 79: 5783–5787, 1982.
Duncan CJ, Publicover SJ: Inhibitory effects of cholinergic agents on the release of transmitter at the frog neuromuscular junction. J. Physiol. 294: 91–103, 1979.
Miyamoto MD: The actions of cholinergic drugs on motor nerve terminals. Pharmacol. Rev. 29: 221–247, 1978.
Rahamimoff R, Yaari Y: Delayed release of transmitter at the frog neuromuscular junction. J. Physiol. 228: 241–257, 1973.
Balnave RJ, Gage PW: On facilitation of transmitter release at the toad neuromuscular junction. J. Physiol. 239: 657–675, 1974.
Datyner NB, Gage PW: Phasic secretion of acetyloholine at a mammalian neuromuscular junction. J. Physiol. 303: 299–314, 1980.
Kita K, Narita K, Van der Kloot W: Effects of temperature on the decline in miniature end-plate potential frequency following a tetanus. Brain Res. 190: 435–445, 1980.
Duncan CJ, Statham HE: Interacting effects of temperature and extracellular calcium on the spontaneous release of transmitter at the frog neuromuscular junction. J. Physiol. 268: 319–333, 1977.
Barrett EF, Barrett JN, Botz D, Chang DB, Mahaffey D: Temperature-sensitive aspects of evoked and spontaneous transmitter release at the frog neuromusoular junction. J. Physiol. 279: 253–273, 1978.
Publicover SJ, Duncan CJ: Dantrolene and the effect of temperature on the spontaneous release of transmitter at the frog neuromuscular junction. Experientia 37: 859–860, 1981.
Hubbard JI, Jones SF, Landau EM: The effect of temperature change upon transmitter release, facilitation and post-tetanic potentiation. J. Physiol. 216: 591–609, 1971.
Ward D, Crowley WJ, Johns TR: Effect of temperature at the neuromuscular junction. Am. J. Physiol. 222: 216–219, 1972.
Duncan CJ: Role of calcium in triggering the release of transmitters at the neuromuscular junction. Cell Calcium 4: 171–193, 1983.
Erulkar SD: The modulation of neurotransmitter release at synaptic junctions. Rev. Physiol. Bioohem. Pharmacol. 98: 64–175, 1983.
Carlen PL, Kosower EM, Werman R: The thiol-oxidizing agent diamide increases transmitter release by decreasing calcium requirements for neuromuscular transmission in the frog. Brain Res. 117: 257–276, 1976.
Kita H, Van der Kloot W: Action of Co and Ni at the frog neuromuscular junction. Nature, New Biol. 245: 52–53, 1973.
Cull-Candy SG, Lundh H, Thesleff S: Effects of botulinum toxin on neuromuscular transmission in the rat. J. Physiol. 260: 177–203, 1976.
Molgo J, Lemeignan M, Lechat P: Effects of 4-aminopyridine at the frog neuromuscular junction. J. Pharmacol. Exp. Therap. 203: 653–663, 1977.
Colomo F, Rahamimoff R: Interaction between sodium and calcium ions in the process of transmitter release at the neuromuscular junction. J. Physiol. 198: 203–218, 1968.
Landau EM, Nachshen DA: The interaction of pH and divalent cations at the neuromuscular junction. J. Physiol. 251: 775–790, 1975.
Manalis RS, Cooper GP, Pomeroy SL: Effects of lead on neuromuscular transmission in the frog. Brain Res. 294: 95–109, 1984.
Pickett JB, Bornstein JC: Some effects of lead at mammalian neuromuscular junction. Am. J. Physiol. 246: C271–C276, 1984.
Uchiyama T, Molgó J, Lemeignan M: Presynaptic effects of bekanamycin at the frog neuromuscular junction. Reversibility by calcium and aminopyridines. Eur. J. Pharmacol. 72: 271–280, 1981.
Pecot-Dechavassine M: Action of vinblastine on the spontaneous release of acetylcholine at the frog neuromuscular junction. J. Physiol. 261: 31–48, 1976.
Dreyer F, Schmitt A: Transmitter release in tetanus and botulinum A toxin-poisoned mammalian motor endplates and its dependence on nerve stimulation and temperature. Pflugers Arch. 399: 228–234, 1983.
Kita H, Narita K, Van der Kloot W: The relation between tonicity and impulse-evoked transmitter release in the frog. J. Physiol. 325: 213–222, 1982.
Rubin LL, Gorio A, Mauro A: Effect of concanavalin A on black widow spider venom activity at the neuromuscular junction: implications for mechanisms of venom action. Brain Res. 143: 107–124, 1978.
Gorio A, Mauro A: Reversibility and mode of action of black widow spider venom on the vertebrate neuromuscular junction. J. Gen. Physiol. 73: 245–263, 1979.
Burgoyne RD, Geisow MJ, Barron J: Dissection of stages in exocytosis in the adrenal chromaffin cell with use of trifluoperazine. Proc. Roy. Soc. Lond. B 216: 111–115, 1982.
Publicover SJ: Presynaptic action of trifluoperazine at the frog neruromuscular junction. N.S. Arch. Pharmacol. 322: 83–88, 1983.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Martinus Nijhoff Publishing, Boston
About this chapter
Cite this chapter
Duncan, C.J. (1986). The Role of Calcium in Triggering the Release of Transmitter at the Vertebrate Neuromuscular Junction. An Analysis of the Effects of Drugs. In: Rahamimoff, R., Katz, B. (eds) Calcium, Neuronal Function and Transmitter Release. Topics in the Neurosciences, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2307-5_22
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2307-5_22
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9420-7
Online ISBN: 978-1-4613-2307-5
eBook Packages: Springer Book Archive