Summary
The metastatic behavior of 14 cell lines derived from 13 individual human tumor cell lines (six of which were colon carcinomas) have been assessed in athymic, nude mice. Many of these lines produced lung metastases after intravenous injection (experimental metastasis) and subcutaneous injection (spontaneous metastasis) into nude mice; however, the most dramatic expression of metastatic behavior was obtained after the injection of tumor cells into the spleens of recipient mice. With this route of cell inoculation, large tumor deposits frequently were found in the liver, the lungs, and the mesenteric and omental lymph nodes. Injection of human tumor lines into the spleens of athymic, nude mice appears to be a novel way of ascertaining the malignant capacity of neoplastic cells.
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References
Hart IR: The role of animal models in the study of experimental metastasis. In: LA Liotta and IR Hart (eds). Tumor invasion and metastasis. Martinus Nijhoff, The Hague, 1982, pp 1–14.
Fogh J, Fogh JM, Orfeo T: One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Natl Cancer Inst 59: 221–226, 1977.
Fogh J, Orfeo T, Tiso J, Sharkey FE, Fogh JM, Daniels WP: Twenty-three new human tumor lines established in nude mice. Exp Cell Biol 48: 229–239, 1980.
Kanazaki T, Hashimoto K, Bath DW: Heterotransplantation of human malignant melanoma cell lines in athymic nude mice. J Natl Cancer Inst 62: 1131–1153, 1979.
Giovanella BC, Stehlin J, Williams LJ Jr: Heterotransplantation of human malignant tumors in “nude” thymusless mice. II. Malignant tumors induced by injection of cell cultures derived from solid tumors. J Natl Cancer Inst 52: 921–930, 1974.
Sordat B, Pritsche R, Mach JP, Carrel S, Ozzello L, Cerotini 3Cs Morphologic and functional evaluation of human solid tumors serially transplanted in nude mice. In: J Rygaard and CO Povlsen (eds), Proceedings of the First International Workshop on nude mice. Gustav-Fisher Verlag, Stuttgart, 1974, pp 269–277.
Sharkey FE, Fogh J: Metastasis of human tumors in athymic nude mice. Int J Cancer 24: 733–738, 1979.
Sordat BC, Ueyama Y, Fogh J: Metastasis of tumor xenografts in the nude mouse. In: J Fogh and BC Giovanella (eds). The nude mouse in experimental and clinical research. Vol 2. Academic Press, New York, 1982, pp 95–143.
Kozlowski JM, Fidler IJ, Campbell D, Xu Z-L, Kaighn ME, Hart IR: Metastatic behavior of human tumor cell lines grown in the nude mouse. Cancer Res 44: 3522–3529, 1984.
Kozlowski JM, Hart IR, Fidler IJ, Hanna N: A human melanoma line heterogeneous with respect to metastatic capacity in athymic nude mice. J Natl Cancer Inst 72: 913–917, 1984.
Fidler 13: Selection of successive tumor lines for metastases. Nature 242: 148–149, 1973.
DeVore DO, Houches DP, Overjera AA, Dill GS, Hutson TB: Collagenase inhibitors retarding invasion of a human tumor in nude mice. Exp Cell Biol 48: 367–373, 1980.
Takahashi S, Konishi Y, Nakatoni K, Inui S, Kojima K, Shiratori T: Conversion of a poorly differentiated human adenocarcinoma to ascites form with invasion and metastasis in nude mice. J Natl Cancer Inst 60: 925–929, 1978.
Witte PL, Ber R: Improved efficiency of hybridoma ascites production by intrasplenic inoculation in mice. J Natl Cancer Inst 70: 575–577, 1983.
Vezeridis MP, Meinmer PA, Kajiji SM, Turner MD, Weimann MC, Calabresi P: Hepatic metastases of human tumor cell lines in nude mice (Abstract). Clin Res 32: 423, 1984.
Kerbel RS, Man MS, Dexter D: A model of human cancer metastasis: Extensive spontaneous and artificial metastasis of a human pigmented melanoma and derived variant sublines in nude mice. J Natl Cancer Inst 72: 93–108, 1984.
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© 1986 Martinus Nijhoff Publishing, Boston
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Hart, I.R. (1986). The Use of Nude Mice to Ascertain the Malignant Capacity of Human Colon Cancer. In: Mastromarino, A.J. (eds) Biology and Treatment of Colorectal Cancer Metastasis. Developments in Oncology, vol 42. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2301-3_2
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DOI: https://doi.org/10.1007/978-1-4613-2301-3_2
Publisher Name: Springer, Boston, MA
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