Abstract
The antigenic heterogeneity of human glioma tissue (19 glioblastoma, 6 astrocytomas) and 13 human glioma-derived cell lines (HGL) was investigated with a panel of 20 monoclonal antibodies (MAs) which defined markers of glioma-associated (GL) or lymphoid distribution (LM). Results obtained from radioimmuno-assay, immunofluorescence, immunohistochemistry, absorption, and polyacrylamide gel analysis estab-lished the following: 1) In general, expression of multiple antigens within one system (GL) correlated with multiple antigen expression in the other (LM). 2) Within the LM panel, Thy-1 and HLA-A,B antigens were expressed by all HGL, whereas only 4/13 HGL were Ia positive, and 3/13 CALLA positive. T-cell marker expression was infrequent and sporadic. 3) Glioblastoma and astrocytoma tissues exhibited less hetero-geneity than HGL; high proportions expressed >75% of the GL specificities tested for. 4) Two MAs highly reactive with glioblastomas (>70% positive) were minimally reactive with astrocytomas (1/6). Glio-blastomas and HGL are antigenically complex and heterogeneous, but the identification of finite antigen distributions by large numbers of MAs may serve a prognostic significance.
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References
Wikstrand CJ, Bigner SH, Bigner DD: Demonstration of complex antigenic heterogeneity in a human glioma cell line and eight derived clones by specific monoclonal antibodies. Cancer Res 43: 3327–3334, 1983
Carrel S, de Tribolet N, Gross N: Expression of HLA-DR and common acute lymphoblastic leukemia antigens on glioma cells. Eur J Immunol 12: 354–357, 1982
Seeger RC, Danon YL, Rayner SA, Hoover F: Definition of a THY-1 determinant of human neuroblastoma, glioma, sarcoma, and teratoma cells with a monoclonal antibody. J Immunol 128: 983–989, 1982
Bigner DD, Bigner SH, Pontén J, Westcrmark B, Mahaley MS, Ruoslanti E, Herschman H, Eng LF, Wikstrand CJ: Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas. J Neuropath Exp Neurol 40: 201–229, 1981
Wikstrand CJ, Bigner DD: Expression of human fetal brain antigens by human tumors of neuroectodermal origin as defined by monoclonal antibodies. Cancer Res 42: 267–275, 1982
Wikstrand CJ, Grahmann FC, McComb RD, Bigner DD: Antigenic heterogeneity of human anaplastic gliomas and glioma-derived cell lines defined by monoclonal antibodies. J Neuropath Exp Neurol 44: 229–241, 1985
Festenstein H, Schmidt W: Variation in MHC antigenic profiles of tumour cells and its biological effects. Immunol Rev 60: 85–127, 1981
Riethmüller G, Johnson J, Wank R et al.: HLA-DR expression on nonlymphoid human tumor cells: biochemical and histochemical studies with monoclonal antibodies. In: GL Wright (ed) Monoclonal Antibodies and Cancer. Academic Press, Inc, New York, 1983, pp 243–249
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© 1986 Martinas Nijhoff Publishers, Boston
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Wikstrand, C.J., Grahmann, F.C., McComb, R.D., Bigner, D.D. (1986). Antigenic heterogeneity of human glioma tissue and cell lines (HGL) defined by monoclonal antibodies (MAs). In: Walker, M.D., Thomas, D.G.T. (eds) Biology of Brain Tumour. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2297-9_69
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DOI: https://doi.org/10.1007/978-1-4613-2297-9_69
Publisher Name: Springer, Boston, MA
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