Antigenic heterogeneity of human glioma tissue and cell lines (HGL) defined by monoclonal antibodies (MAs)
The antigenic heterogeneity of human glioma tissue (19 glioblastoma, 6 astrocytomas) and 13 human glioma-derived cell lines (HGL) was investigated with a panel of 20 monoclonal antibodies (MAs) which defined markers of glioma-associated (GL) or lymphoid distribution (LM). Results obtained from radioimmuno-assay, immunofluorescence, immunohistochemistry, absorption, and polyacrylamide gel analysis estab-lished the following: 1) In general, expression of multiple antigens within one system (GL) correlated with multiple antigen expression in the other (LM). 2) Within the LM panel, Thy-1 and HLA-A,B antigens were expressed by all HGL, whereas only 4/13 HGL were Ia positive, and 3/13 CALLA positive. T-cell marker expression was infrequent and sporadic. 3) Glioblastoma and astrocytoma tissues exhibited less hetero-geneity than HGL; high proportions expressed >75% of the GL specificities tested for. 4) Two MAs highly reactive with glioblastomas (>70% positive) were minimally reactive with astrocytomas (1/6). Glio-blastomas and HGL are antigenically complex and heterogeneous, but the identification of finite antigen distributions by large numbers of MAs may serve a prognostic significance.
Keywordsheterogeneity gliomas monoclonal antibodies
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