Abstract
A variety of morphological and physiological techniques have been applied to a transplantable rat glioma model in an attempt to elucidate details of vascular development and transport phenomena. Three mor-phological stages of tumour vascular development are defined. General vascularity has been quantitatively assessed using light microscopy of paraffin and resin-embedded material. Fenestrations, abnormal junctions and vesicular profiles have been counted in normal and tumour vessels to provide a quantitative assessment of these recognised ultrastructural abnormalities of glioma vasculature. Changes in the numerical density of sectioned vascular profiles and in the size of vessels have been measured in consecutive sample areas extending from brain surrounding tumour towards the tumour centre.
Intravenous and cerebral intraventricular injections of horseradish peroxidase (HRP) were used to demonstrate differences in the transport of a large hydrophilic molecule by vessels within tumours and by those of peritumoral tissue. To quantify the kinetics of transfer of a small polar molecule into tumours and adjacent brain steady plasma levels of [14C] mannitol were maintained for increasing periods of time in separate tumour-bearing and control animals. The ratio of tissue to plasma radioactivity was plotted against time and the apparent transfer constants for mannitol calculated: that for entry into tumour tissue was approximately 80 times that into normal brain.
These studies aim to solve clinical problems including those of cerebral oedema and of drug delivery to gliomas.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Davaki P, Lantos PL: The development of brain tumours in rats by the intracerebral injection of neoplastic glial cells: A fine structural study. Neuropathol Appl Neurobiol 7: 49–61, 1981
Deane BR, Lantos PL: The vasculature of experimental brain tumours, Part 1 (A sequential light and electron mi-croscopic study of angiogenesis). J Neurol Sci 49: 55–66, 1981
Deane BR, Lantos PL: The vasculature of experimental brain tumours. Part 2 (A quantitative assessment of mor-phological abnormalities). J Neurol Sci 49: 67–77, 1981
Deane BR, Papp MI, Lantos PL: The vasculature of experi-mental brain tumours, Part 3 (Permeability studies). J Neu-rol Sci 65: 47–58, 1984
Deane BR, Greenwood J, Lantos PL, Pratt OE: The vas-culature of experimental brain tumours.,Part 4 (The quan-tification of vascular permeability). J Neurol Sci 65: 59–68, 1984
Lantos PL, Roscoe JP, Skidmore CJ: Studies of the mor-phology and tumourogenicity of experimental brain tu-mours in tissue culture. Br J Exp Pathol 57: 95–104, 1976
Brem S, Cotran R, Folkman J: Tumor angiogenesis — A quantitative method for histologie grading. J Natl Cancer Inst 48: 347–356, 1972
Graham Jr, RC, Karnovsky MJ: The early stages of absorp-tion of injected horseradish peroxidase in the proximal tubules of mouse kidney — Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14: 291–302, 1966
Pratt OE: Continuous-injection methods for the measure-ment of flux across the blood-brain barrier. The steady-state, initial-rate method. Res Methods Neurochem 6: 117–150, 1985
Weiler RO, Griffin RL: Transmission and scanning elec-tron microscopy of the microcirculation of gliomas. In: J Cervos-Navarro, E Betz, G Eblardt, R Ferszt, R Wullen-weber (eds) Pathology of cerebrospinal microcirculation. Adv Neurol 20: 569–575. 1978
Luthert PJ, Lantos PL: A morphometric study of the micro-vasculature of a rat glioma. Neuropathol Appl Neurobiol 11: 461–473. 1985
Hurter T, Mennel HD: Experimental brain tumours and edema in rats, Part 1 (Histology and cytology of tumors). Acta Neuropathol (Bed) 55: 105–111, 1981
Groothuis DR, Fisher HM, Lapin G, Bigner DD, Vick NA: Permeability of different experimental brain tumor models to horseradish peroxidase. J Neuropathol Exp Neurol 41: 164–185, 1982
Blasberg RG, Shapiro WR, Molnar P, Patlak CS, Fenster-macher JD: Local blood-to-tissue transport in Walker 256 metastatic brain tumors. J Neuro-Oncol 2: 205–218, 1984
Waggener JD, Beggs JL: Vasculature of neural neoplasms. In: RA Thompson and JR Green (eds) Neoplasia in the central nervous system. Adv Neurol 15: 27–49, 1976
Hirano A, Matsui T: Vascular structures in brain tumors. Hum Pathol 6: 611–621, 1975
Bradbury MWB: The concept of a Blood-Brain Barrier. John Wiley and Sons, Chichester, New York, Brisbane, Toronto. 1979
Simionescu N: Cellular aspects of transcapillary exchange. Phys Rev 63: 1536–1579, 1983
Loudon MF, Michel CC, White IF: The labelling of frog endothelial cells with ferritin. J Physiol (Lond) 296: 97–112, 1979
Renkin EM, Curry FE: Endothelial permeability: Path-ways and modulations. In: AP Fishman (ed) Endothelium. Annals NY Acad Sci 401: 248–258, 1982
McGuire PG, Twietmeyer TA: Morphology of rapidly frozen aortic endothelial cells. Glutaraldehyde fixation in-creases the number of caveolae. Circ Res 53: 424–429, 1983
Wagner RC, Casley-Smith JR: Endothelial vesicles. Micro-vase Res 21: 267–298, 1981
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Martinas Nijhoff Publishers, Boston
About this chapter
Cite this chapter
Luthert, P.J., Deane, B.R., Greenwood, J., Pratt, O.E., Lantos, P.L. (1986). The vasculature of experimental brain tumours: angiogenesis, vascular pathology and permeability studies. In: Walker, M.D., Thomas, D.G.T. (eds) Biology of Brain Tumour. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2297-9_27
Download citation
DOI: https://doi.org/10.1007/978-1-4613-2297-9_27
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9415-3
Online ISBN: 978-1-4613-2297-9
eBook Packages: Springer Book Archive