Immunohistochemical demonstration of HLA-DR positive cells in normal and neoplastic brain tissues. I. Preliminary Report

  • Marco Chilosi
  • Massimo Gerosa
  • Antonio Iannucci
  • Mark Rosenblum
  • Claudio Doglioni
  • Mara Montagna
  • Gabriella Stevanoni
  • Albino Bricolo
  • Giuseppe Tridente

Abstract

Recent evidence indicates that HLA-DR positive cells of putatively glial origin may be present in normal and neoplastic brain tissues. The nature and function of these cells is under debate. Freshly frozen sections from 9 brain tumor patients were analyzed by immunohistochemical techniques for HLA-DR antigens, using a panel of antisera and monoclonal antibodies, with and without normal human serum pre-incubation; five non-neoplastic brain and cerebellum samples served as control. All normal and tumoral specimens investigated showed a spectrum of HLA-DR positivity. A marked heterogeneity in HLA-DR expression was observed in gliomas: the majority of cells with typical neoplastic morphology were devoid of immunostaining, whereas a smaller amount (10–25%) of predominantly astro-cyte-like elements proved to be HLA-DR positive. In a proportion of these cells, strong reactivity was shown by 2D1 and LC, two different monoclonal àntibodies which are considered to be specific for bone-marrow-derived leukocytes and histiocytes. Normal human serum pre-incubation had no significant effect on HLA-DR, GFAP, 2D1 and LC staining; however serum pre-incubation was followed by partial or complete inhibition for OKT6, OKT4 and OKT8 binding.

Keywords

brain brain tumor glioma histocompatibility antigens HLA-DR immunohistochemistry 

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Copyright information

© Martinas Nijhoff Publishers, Boston 1986

Authors and Affiliations

  • Marco Chilosi
    • 1
    • 2
  • Massimo Gerosa
    • 1
    • 2
  • Antonio Iannucci
    • 1
    • 2
  • Mark Rosenblum
    • 1
    • 2
  • Claudio Doglioni
    • 1
    • 2
  • Mara Montagna
    • 1
    • 2
  • Gabriella Stevanoni
    • 1
    • 2
  • Albino Bricolo
    • 1
    • 2
  • Giuseppe Tridente
    • 1
    • 2
  1. 1.Departments of Pathology, Immunology and NeurosurgeryUniversity of VeronaItaly
  2. 2.Brain Tumor Research CenterUniversity of CaliforniaSan FranciscoUSA

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