Immunohistochemical demonstration of HLA-DR positive cells in normal and neoplastic brain tissues. I. Preliminary Report
Recent evidence indicates that HLA-DR positive cells of putatively glial origin may be present in normal and neoplastic brain tissues. The nature and function of these cells is under debate. Freshly frozen sections from 9 brain tumor patients were analyzed by immunohistochemical techniques for HLA-DR antigens, using a panel of antisera and monoclonal antibodies, with and without normal human serum pre-incubation; five non-neoplastic brain and cerebellum samples served as control. All normal and tumoral specimens investigated showed a spectrum of HLA-DR positivity. A marked heterogeneity in HLA-DR expression was observed in gliomas: the majority of cells with typical neoplastic morphology were devoid of immunostaining, whereas a smaller amount (10–25%) of predominantly astro-cyte-like elements proved to be HLA-DR positive. In a proportion of these cells, strong reactivity was shown by 2D1 and LC, two different monoclonal àntibodies which are considered to be specific for bone-marrow-derived leukocytes and histiocytes. Normal human serum pre-incubation had no significant effect on HLA-DR, GFAP, 2D1 and LC staining; however serum pre-incubation was followed by partial or complete inhibition for OKT6, OKT4 and OKT8 binding.
Keywordsbrain brain tumor glioma histocompatibility antigens HLA-DR immunohistochemistry
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- 4.Chilosi M, Bonetti F, Iannucci A: Carcinogenic 3′3-di-aminobenzidine (letter). Am J Clin Pathol 75: 638, 1981Google Scholar
- 9.Fontana A, Kristensen F, Dubs R, Gemsa D, Weber E: Production of prostaglandin E and an interleukin-1 like factor by cultured astrocytes and C6 glioma cells. J Immu-nol 129: 2413–2419, 1982Google Scholar