Skip to main content
SpringerLink
Log in

Inactivation of Viral Promoters by In Vitro Methylation: Studies on the Adenovirus and Baculovirus System

  • Chapter
Book cover Adenovirus DNA

Part of the book series: Developments in Molecular Virology ((DMVI,volume 8))

  • 68 Accesses

Abstract

Frequently, viruses have served a very important role in investigations on the mechanisms involved in gene expression in eukaryotic cells. For example, the principle of RNA splicing was first recognized during the course of studies on gene expression in adenovirus- and simian virus 40-infected cells (e.g. 1, 2). Using the same viral systems, decisive progress was made in developing methods to map viral transcripts and to employ cellfree transcription (3) and splicing assays (4) in order to elucidate details of these mechanisms. For several years, we have studied the functional significance of site-specific DNA methylations, particularly in the promoter part of adenovirus genes by a number of different experimental approaches. Again, the adenovirus system offered several advantages as an experimental tool, not only due to the manageable size of its genome and the wealth of information available on the molecular biology of these viruses (5). In addition, the interaction of the adenoviral genome with host cell factors could be analyzed in quite different biological environments, i.e. in productively or abortively infected cells, as well as in adenovirus-transformed cells in whose genome the adenovirus genome was stably integrated (6 – 9).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Berget, S.M., Moore, C. and Sharp, P.A. Proc. Natl. Acad. Sci. USA 74: 3171–3175, 1977.

    Article  PubMed  CAS  Google Scholar 

  2. Aloni, Y., Dhar, R., Laub, O., Horowitz, M. and Khoury, G. Proc. Natl. Acad. Sci. USA 74: 3686–3690, 1977.

    Article  PubMed  CAS  Google Scholar 

  3. Manley, J.L., Fire, A., Cano, A., Sharp, P.A. and Gefter, M.L. Proc. Natl. Acad. Sci. USA 17: 3855–3859, 1980.

    Article  Google Scholar 

  4. Hernandez, N. and Keller, W. Cell 35: 89–99, 1983.

    Article  PubMed  CAS  Google Scholar 

  5. The Molecular Biology of Adenoviruses 1–3, ed. W. Doerfler. In: Current Topics Microbiol. Immunol. 109 (1983), 110 (1984), 111 (1984).

    Google Scholar 

  6. Doerfler, W. Proc. Natl. Acad. Sci. USA 60: 636–643, 1968.

    Article  PubMed  CAS  Google Scholar 

  7. Doerfler, W. J. Virol. 6: 652–666, 1970.

    PubMed  CAS  Google Scholar 

  8. Doerfler, W. Current Topics Microbiol. Immunol. 101: 127–194, 1982.

    Article  CAS  Google Scholar 

  9. Doerfler, W., Gahlmann, R., Stabel, S., Deuring, R., Lichtenberg, U., Schulz, M., Eick, D. and Leisten, R. Current Topics Microbiol. Immunol. 109: 193–228, 1983.

    Article  CAS  Google Scholar 

  10. Günthert, U., Schweiger, M., Stupp, M. and Doerfler, W. Proc. Natl. Acad. Sci. USA 73: 3923–3927, 1976.

    Article  PubMed  Google Scholar 

  11. Von Acken, U., Simon, D., Grunert, F., Döring, H.A. and Kröger, H. Virology 99: 152–157, 1979.

    Article  Google Scholar 

  12. Eick, D., Fritz, H.-J. and Doerfler, W. Anal. Biochem. 135: 165–171, 1983.

    Article  PubMed  CAS  Google Scholar 

  13. Vardimon, L., Neumann, R., Kuhlmann, I., Sutter, D. and Doerfler, W. Nucleic Acids Res. 8: 2461–2473, 1980.

    Article  PubMed  CAS  Google Scholar 

  14. Wienhues, U. and Doerfler, W. J. Virol. 57: 000–000, 1985.

    Google Scholar 

  15. Deuring, R., Klotz, G. and Doerfler, W. Proc. Natl. Acad. Sci. USA 78: 3142–3146, 1981.

    Article  PubMed  CAS  Google Scholar 

  16. Deuring, R. and Doerfler, W. Gene 26: 283–289, 1983.

    Article  PubMed  CAS  Google Scholar 

  17. Sutter, D. and Doerfler, W. Cold Spring Harbor Symp. Quant. Biol. 44: 565–568, 1979.

    Google Scholar 

  18. Sutter, D. and Doerfler, W. Proc. Natl. Acad. Sci. USA 77: 253–256, 1980.

    Article  PubMed  CAS  Google Scholar 

  19. Kruczek, I. and Doerfler, W. EMBO J. 1: 409–414, 1982.

    PubMed  CAS  Google Scholar 

  20. Kuhlmann, I. and Doerfler, W. Virology 118: 169–180, 1982.

    Article  PubMed  CAS  Google Scholar 

  21. Kuhlmann, I., Achten, S., Rudolph, R. and Doerfler, W. EMBO J. 1 79–86, 1982.

    PubMed  CAS  Google Scholar 

  22. Kuhlmann, I. and Doerfler, W. J. Virol. 47: 631–636, 1983.

    PubMed  CAS  Google Scholar 

  23. Doerfler, W., Kruczek, I., Eick, D., Vardimon, L. and Kron, B. Cold Spring Harbor Symp. Quant. Biol. 47: 593–603, 1982.

    CAS  Google Scholar 

  24. Knust-Kron, B., Hübscher, U., Eick, D., Spadari, S. and Doerfler, W., submitted, 1985.

    Google Scholar 

  25. Hübscher, U., Pedrali-Nay, G., Knust-Kron, B., Doerfler, W. and Spadari, S. Anal. Biochem., submitted, 1985.

    Google Scholar 

  26. Razin, A. and Riggs, A.D. Science 210: 604–610, 1980.

    Article  PubMed  CAS  Google Scholar 

  27. Doerfler, W. J. gen. Virol. 57: 1–20, 1981.

    Article  PubMed  CAS  Google Scholar 

  28. Ehrlich, M. and Wang, R.Y.-H. Science 212: 1350–1357, 1981.

    Article  PubMed  CAS  Google Scholar 

  29. Razin, A. and Friedmann, J. Progr. Nucleic Acids Res. Mol. Biol. 25: 33–52, 1981.

    Article  CAS  Google Scholar 

  30. Doerfler, W. Ann. Rev. Biochem. 52: 93–124, 1983.

    Article  PubMed  CAS  Google Scholar 

  31. Cooper, D.N. Human Genetics 64: 315–333, 1983.

    Article  PubMed  CAS  Google Scholar 

  32. Riggs, A.D. and Jones, P.A. Adv. Cancer Res. 40: 1–30, 1983.

    Article  PubMed  CAS  Google Scholar 

  33. Doerfler, W. Adv. Viral Oncol. 4: 217–247, 1984.

    CAS  Google Scholar 

  34. Doerfler, W. Current Topics Microbiol. Immunol. 108: 79–98, 1984.

    CAS  Google Scholar 

  35. Trautner, T.A. (ed.) Methylation of DNA. Springer, Berlin/Heidelberg/New York/Tokyo, 1984.

    Google Scholar 

  36. Taylor, J.H. DNA Methylation and Cellular Differentiation. Springer, Wien/New York, 1984.

    Google Scholar 

  37. Razin, A., Cedar, H., Riggs, A.D. (eds.) DNA Methylation. Springer, New York, 1984.

    Chapter  Google Scholar 

  38. Hattman, S. DNA Methylation. In: The Enzymes (Ed. P.D. Boyer), Academic Press, New York, 1984.

    Google Scholar 

  39. Sutter, S., Westphal, M. and Doerfler, W. Cell 14: 569–585, 1978.

    Article  PubMed  CAS  Google Scholar 

  40. Stabel, S., Doerfler, W. and Friis, R.R. J. Virol. 36: 22–40, 1980.

    PubMed  CAS  Google Scholar 

  41. Ortin, J., Scheidtmann, K.-H., Greenberg, R., Westphal, M. and Doerfler, W. J. Virol. 20: 355–372, 1976.

    PubMed  CAS  Google Scholar 

  42. Carstens, E.B., Tjia, S.T. and Doerfler, W. Virology 99: 386–398, 1979.

    Article  PubMed  CAS  Google Scholar 

  43. Tjia, S.T., Carstens, E.B. and Doerfler, W. Virology 99: 399–409, 1979.

    Article  PubMed  CAS  Google Scholar 

  44. Lübbert, H. and Doerfler, W. J. Virol. 50: 497–506, 1984.

    PubMed  Google Scholar 

  45. Lübbert, H. and Doerfler, W. J. Virol. 52: 255–265, 1984.

    PubMed  Google Scholar 

  46. Smith, S.S. and Thomas, C.A., Jr. Gene 13: 395–408, 1981.

    Article  PubMed  CAS  Google Scholar 

  47. Urieli-Shoval, S., Gruenbaum, Y., Sedat, J. and Razin, A. FEBS Letters 146: 148–152, 1982.

    Article  PubMed  CAS  Google Scholar 

  48. Müller, T. and Doerfler, W., unpublished results, 1985.

    Google Scholar 

  49. Achwal, C.W., Ganguly, P. and Chandra, H.S. EMBO J. 3: 263–266, 1984.

    PubMed  CAS  Google Scholar 

  50. Hooft van Iddekinge, B.J.L., Smith, G.E. and Summers, M.D. Virology 131: 561–565, 1983.

    Article  PubMed  CAS  Google Scholar 

  51. Rohel, D.Z., Cochran, M.A. and Faulkner, P. Virology 124: 357–365, 1983.

    Article  PubMed  CAS  Google Scholar 

  52. The Molecular Biology of Baculoviruses, (ed.) W. Doerfler. In: Current Topics Microbiol. Immunol., in preparation.

    Google Scholar 

  53. Kaye, A.M. and Winocour, E. J. Mol. Biol. 24: 475–478, 1967.

    Article  CAS  Google Scholar 

  54. Willis, D.B. and Granoff, A. Virology 102: 250–257, 1980.

    Article  Google Scholar 

  55. Kruczek, I. and Doerfler, W. Proc. Natl. Acad. Sci. USA 80: 7586–7590, 1983.

    Article  PubMed  CAS  Google Scholar 

  56. Doerfler, W. Virology 18: 587–606, 1969.

    Article  Google Scholar 

  57. Vardimon, I. and Doerfler, W. J. Mol. Biol. 141: 227–246, 1981.

    Article  Google Scholar 

  58. Cook, J.L. and Lewis, A.M., Jr. Cancer Res. 39: 1455–1461, 1979.

    PubMed  CAS  Google Scholar 

  59. Vardimon, L., Renz, D. and Doerfler, W. Recent Results Cancer Res. 84: 90–102, 1983.

    PubMed  CAS  Google Scholar 

  60. Esche, H. J. Virol. 41: 1076–1082, 1982.

    PubMed  CAS  Google Scholar 

  61. Johansson, K., Persson, H., Lewis, A.M., Pettersson, U., Tibbetts, C. and Philipson, L. J. Virol. 22: 628–639, 1978.

    Google Scholar 

  62. Van der Vliet, P.C. and Levine, A.J. Nature New Biol. 246: 170–174, 1973.

    PubMed  Google Scholar 

  63. Vardimon, L., Gunthert, U. and Doerfler, W. Mol. Cell. Biol. 2: 1574–1580, 1982.

    PubMed  CAS  Google Scholar 

  64. Church, G.M. and Gilbert, W. Proc. Natl. Acad. Sci. USA 81: 1991–1995, 1984.

    Article  PubMed  CAS  Google Scholar 

  65. Josse, J., Kaiser, A.D. and Romberg, A. J. Biol. Chem. 236: 864–875, 1961.

    CAS  Google Scholar 

  66. Subak-Sharpe, H., Burk, R.R., Crawford, L.V., Morrison, J.M., Hay, J. and Keir, H.M. Cold Spring Harbor Symp. Quant. Biol. 31: 737–748, 1966.

    PubMed  CAS  Google Scholar 

  67. Morrison, J.M., Keir, H.M., Subak-Sharpe, H. and Crawford, L.V. J. gen. Virol. 1: 101–108, 1967.

    Article  PubMed  CAS  Google Scholar 

  68. Felsenfeld, G., Nickol, J., Behe, M., Mhee, J. and Jackson, D. Cold Spring Harbor Symp. Quant. Biol. 47: 577–584, 1982.

    CAS  Google Scholar 

  69. Tykocinski, M.L. and Max, E.E. Nucleic Acids Res. 12: 4385–4396, 1984.

    Article  PubMed  CAS  Google Scholar 

  70. Waalwijk, C. and Flavell, R.A. Nucleic Acids Res. 5: 3231–3236, 1978.

    Article  PubMed  CAS  Google Scholar 

  71. Southern, E.M. J. Mol. Biol. 98: 503–517, 1975.

    Article  CAS  Google Scholar 

  72. Vardimon, L., Kuhlmann, I., Cedar, H., and Doerfler, W. Eur. J. Cell Biol. 25: 13–15, 1981.

    PubMed  CAS  Google Scholar 

  73. Vardimon, L., Kressmann, A., Cedar, H., Maechler, H. and Doerfler, W. Proc. Natl. Acad. Sci. USA 79: 1073–1077, 1982.

    Article  PubMed  CAS  Google Scholar 

  74. Langner, K.-D., Vardimon, L., Renz, D. and Doerfler, W. Proc. Natl. Acad. Sci. USA 81: 2950–2954, 1984.

    Article  PubMed  CAS  Google Scholar 

  75. Roberts, R. J., Akusjärvi, G., Alestrom, P., Gelinas, R.E., Gingeras, T.R., Sciaky, D. and Pettersson, U. Adenovirus DNA: The Viral Genome and its Expression, ed. W. Doerfler. In: Developments in Molecular Virology, Martinus Nijhoff Publishers, Boston, 1985.

    Google Scholar 

  76. Marinus, M.G., Morris, N.R. J. Bacteriol. 114: 1143–1150, 1973.

    PubMed  CAS  Google Scholar 

  77. Gorman, C.M., Moffat, L.F. and Howard, B.H. Mol. Cell. Biol. 2: 1044–1051, 1982.

    PubMed  CAS  Google Scholar 

  78. Langner, K.-D., Weyer, U. and Doerfler, W. Proc. Natl. Acad. Sci. USA 82: 0000–0000, 1985.

    Google Scholar 

  79. Knebel, D. and Doerfler, W. J. Virol., submitted, 1985.

    Google Scholar 

  80. Russlinger, M., Hurst, J. and Flavell, R.A. Cell 24: 197–206, 1983.

    Article  Google Scholar 

  81. Stein, R., Gruenbaum, Y., Pollak, Y., Razin, A. and Cedar, H. Proc. Natl. Acad. Sci. USA 79.:61–65, 1982.

    Google Scholar 

  82. Knebel, D., Lübbert, H. and Doerfler, W. EMBO J. 4: 000–000, 1985.

    CAS  Google Scholar 

  83. Kuzio, J., Rohel, D.Z., Curry, C.J., Krebs, A., Carstens, E.B. and Faulkner, P. Virology, 119: 414–418, 1984.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Genetics, University of Cologne, Cologne, Germany

    W. Doerfler, K.-D. Langner, D. Knebel, H. Lübbert & B. Knust-Kron

Authors
  1. W. Doerfler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K.-D. Langner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Knebel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Lübbert
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Knust-Kron
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Institute of Genetics, University of Cologne, 5000, Cologne 41, Federal Republic of Germany

    Walter Doerfler

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Martinus Nijhoff Publishing, Boston

About this chapter

Cite this chapter

Doerfler, W., Langner, KD., Knebel, D., Lübbert, H., Knust-Kron, B. (1986). Inactivation of Viral Promoters by In Vitro Methylation: Studies on the Adenovirus and Baculovirus System. In: Doerfler, W. (eds) Adenovirus DNA. Developments in Molecular Virology, vol 8. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2293-1_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2293-1_6

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9413-9

  • Online ISBN: 978-1-4613-2293-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation