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Immunobiology and Immunopharmacology of Bacterial Endotoxins pp 89–96Cite as

Analysis and Characterization of Bacterial Endotoxins (Lipopolysaccharides) by SDS-Polyacrylamide Gel Electrophoresis Followed by Silver Stain

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Part of the book series: University of South Florida International Biomedical Symposia Series ((EMISS,volume 18))

Abstract

Lipopolysaccharide (LPS) is a surface component of gram-negative bacteria, and possesses both antigenic and pharmacological properties. The term endotoxin is used synonymously with LPS in emphasizing its toxic properties. Enterobacterial LPS consists of three regions, i.e., lipid A, core and O-side-chain, the latter which may not be present in certain bacteria. The antigenic specificities of LPSs reside primarily in the O-side-chains but also in the core regions, while the biological properties reside in the lipid A moiety (1).

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Authors and Affiliations

  1. Office of Biologics Research and Review, Food and Drug Administation, Bethesda, Maryland, USA

    Chao-Ming Tsai & Carl E. Frasch

Authors
  1. Chao-Ming Tsai
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  2. Carl E. Frasch
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Editor information

Editors and Affiliations

  1. University of South Florida College of Medicine, Tampa, Florida, USA

    Andor Szentivanyi (Dean of the College of Medicine Deputy Vice President for Medical Affairs Professor of Pharmacology and Internal Medicine Chairman of the Department of Pharmacology and Therapeutics) & Herman Friedman (Professor and Chairman) (Dean of the College of Medicine Deputy Vice President for Medical Affairs Professor of Pharmacology and Internal Medicine Chairman of the Department of Pharmacology and Therapeutics) &  (Professor and Chairman)

  2. University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Alois Nowotny

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© 1986 Plenum Press, New York

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Tsai, CM., Frasch, C.E. (1986). Analysis and Characterization of Bacterial Endotoxins (Lipopolysaccharides) by SDS-Polyacrylamide Gel Electrophoresis Followed by Silver Stain. In: Szentivanyi, A., Friedman, H., Nowotny, A. (eds) Immunobiology and Immunopharmacology of Bacterial Endotoxins. University of South Florida International Biomedical Symposia Series, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2253-5_8

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  • DOI: https://doi.org/10.1007/978-1-4613-2253-5_8

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9319-4

  • Online ISBN: 978-1-4613-2253-5

  • eBook Packages: Springer Book Archive

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