Abstract
Thirty years ago in 1954, we summarized our knowledge then of the chemistry and biology of endotoxins (73). At that time, the phenol/water extraction procedure had been established (74), and the extracts from a number of bacterial strains studied chemically and biologically. These investigations had led to the identification of the extracted products as the 0 antigens and endotoxins of these bacteria, resembling products isolated earlier by Boivin and Mesrobeanu (2), Morgan et al. (44), Goebel et al. (15), Shear et al. (62), and others. It had been recognized that endotoxins are composed of a polysaccharide component linked covalently to a lipid component, and thus were chemically lipopolysaccharides. The lipid had been termed lipid A (73) and found to contain D - glucosamine, phosphate, and long-chain fatty acids in ester and amide linkages. It had been shown that the linkage of the polysaccharide to lipid A could be cleaved by hydrolysis with dilute acetic or hydrochloric acid, leading to free water-soluble polysaccharide and free water-insoluble lipid A. Neither free polysaccharide nor free insoluble lipid A expressed endotoxic activities. Nevertheless, it has been anticipated that lipid A was the biologically active part and that activity would result from the binding of lipid A to the polysaccharide component, the latter functioning as a hydrophilic carrier for lipid A.
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References
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Lüderitz, O., Galanos, C., Rietschel, E.T., Westphal, O. (1986). Lipid A: Relationships of Chemical Structure and Biological Activity. In: Szentivanyi, A., Friedman, H., Nowotny, A. (eds) Immunobiology and Immunopharmacology of Bacterial Endotoxins. University of South Florida International Biomedical Symposia Series, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2253-5_5
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