Abstract
Since its discovery in 1968 by Sultzer (1) as a mutant mouse strain which displayed aberrant peritoneal inflammatory cell responses to endotoxin, the C3H/HeJ mouse has served as one of the dominant experimental models by which to define both in vivo and in vitro the mode of action of endotoxin. Extensive studies have documented that the phenotypic characteristic of endotoxin unresponsiveness is specific for the LPS fraction of endotoxin, and more specifically, the lipid A component (reviewed in 2). Further, the available evidence would suggest that the defect responsible for LPS/lipid A unresponsiveness may be a characteristic of all cells derived from this mouse in that lymphocytes, macrophages and fibroblasts are all refractory to LPS stimulation (3). Genetic evidence has clearly linked this mutation to a locus on chromosome 4. This locus appears to be codominantly expressed and LPS unresponsiveness is inherited as a single gene trait in the appropriate F2 backcrosses (5).
Keywords
- Adoptive Transfer
- Mitogenic Activity
- Bacterial Lipopolysaccharide
- Minor Histocompatibility Antigen
- Murine Lymphocyte
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
B. H. Sultzer, Genetic control of leukocyte responses to endotoxin, Nature 219: 1253 (1968).
D. C. Morrison and J. L. Ryan, A review - bacterial endotoxins and host immune responses, in: “Advances in Immunology,” Volume 28, F. J. Dixon and H. G. Kunkel, eds., Academic Press, New York (1979).
D. C. Morrison, Bacterial endotoxins and pathogenesis, in: “Reviews of Infectious Diseases,” 5:S733 (1983).
J. Watson, K. Kelly, M. Largen, and B. A. Taylor, The genetic mapping of a defective LPS response gene in C3H/HeJ mice, J. Immunol. 129: 422 (1978).
J. Watson and R. Riblet, Genetic control of responses to bacterial lipopolysaccharides in mice. I. Evidence for a single gene that influences mitogenic and immunogenic responses to lipopolysaccharides, J. Exp. Med. 140: 1147 (1974).
A. Jakobovits, N. Sharon, and I. Zan-Bar, Acquisition of mitogenic responsiveness by nonresponding lymphocytes upon insertion of appropriate membrane components, J. Exp. Med. 156: 1274 (1982).
H. Nygren, G. Dahlen, and G. Moller, Bacterial lipopolysaccharides bind selectively to lymphocytes from lipopolysaccharide high responder mouse strains, Scand. J. Immunol. 10: 555 (1979).
R. Chaby, R. Metezeau, and R. Girard, Binding of a rhodamine-labelled lipopolysaccharide to lipopolysaccharide-responder and non-responder lymphocytes, Cell Immunol. 85: 531 (1984).
S. A. Goodman and D. C. Morrison, Selective association of lipid-rich R-like lipopolysaccharide subunits with murine spleen cells, Molecular Immunol. 21: 689 (1984).
B. M. Sultzer, Genetic analysis of lymphocyte activation by lipopolysaccharide endotoxin, Infect. Immun. 13: 1579 (1976).
D. M. Jacobs, D. B. Roberts, J. H. Eldridge, and A. J. Rosenspire, Binding of bacterial lipopolysaccharide to murine lymphocytes, Ann. NY Acad. Sci. 409: 72 (1983).
L. Forni and A. Coutinho, An antiserum which recognizes lipopolysaccharide-reactive B cells in the mouse, Eur. J. Immun. 8: 56 (1978).
A. Coutinho, L. Forni, and T. Watanabe, Genetic and functional characterization of an antiserum to the lipid A-specific triggering receptor on murine B lymphocytes, Eur. J. Immun. 8: 63 (1978).
J. Watson, K. Kelly, and C. Whitlock, Genetic control of endotoxin sensitivity, in: “Microbiology,” D. Schlessinger, ed., American Society for Microbiology, Washington, D. C. (1980).
S. W. Vukajlovich and D. C. Morrison, Conversion of lipopolysaccharides to molecular aggregates with uniform subunit composition: demonstration of LPS-responsiveness in “endotoxin-unresponsive” C3H/HeJ B-lymphocytes, J. Immunol. 130: 2804 (1983).
F. C. McIntire, H. W. Sievert, G. H. Barlow, R. A. Finley, and A. Y. Lee, Chemical, physical and biological properties of a lipopolysaccharide from Escherichia coli K-235, Biochem. 6: 2363 (1967).
B. J. Skidmore, D. C. Morrison, J. M. Chiller and W. O. Weigle, Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS, J. Exp. Med. 142: 1488 (1975).
O. Westphal, O. Lüderitz, and F. Bister, Uber der Extraktion von Bakterien mit phenol Wasser, Z. Naturforsch. 7b: 148 (1952).
D. C. Morrison and L. Leive, Fractions of lipopolysaccharide from Escherichia coli 0111:B4 prepared by two extraction procedures, Biol. Chem. 250: 2911 (1975).
C. Galanos, O. Lüderitz, and O. Westphal, A new method for the extraction of R lipopolysaccharides, Eur J. J. Biochem. 9: 245 (1969).
R. J. Ulevitch, The preparation and characterization of a radioiodinated bacterial lipopolysaccharide, Immunochemistry 15: 157 (1978).
U. K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature (London) 227: 680 (1970).
J. A. Rudbach, R. L. Anacher, W. T. Haskins, A. G. Johnson, K. C. Milner, and E. Ribi, Physical aspects of reversible inactivation of endotoxin, Ann. NY Acad. Sci. 133: 629 (1966).
V, S. Waravdekar and L. D. Saslaw, A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of malonaldehyde-thiobarbituric aeid reactions J. Biol Chem. 234: 1945 (1959).
D. C. Morrison, S. J. Betz, and D. M. Jacobs, Isolation of a lipid A bound polypeptide responsible for “LPS-initiated” mitogenesis of C3H/HeJ spleen cells, J. Exp. Med. 144: 840 (1976).
M. H. Julius, E. Simpson, and L. A. Herzenberg, A rapid method for the isolation of functional thymus-derived murine lymphocytes, Eur. J. Immunol. 3: 645 (1973).
L. L. Perry and M. I. Greene, Conversion of immunity to suppression by in vivo administration of I-A subregion specific antibodies, J. Exp. Med. 156: 480 (1982).
W. Fierz, M. Brenan, A. Mullbader, and E. Simpson, Non-H-2 and H-2-linked immune response genes control the cytotoxic T-cell response to H-Y, Immunogenetics 15: 261 (1982).
S. A. Goodman and D.C. Morrison, Lipopolysaccharides receptors on lymphocytes. I. Lack of immunologic recognition of a putative LPS receptor on LPS responder lymphocytes by non-responder mice, J. Immunology, in press (1985).
P. O. Livingston, A. B. DeLeo, M. Jones, and H. F. Oettgen, Comparison of approaches for augmenting the serologic response to the individually specific methylcholanthrene-induced sarcome-neth A: pretreatment with cyclophosphamide is most effective, J. Immunol. 131: 2601 (1983).
S. J. Betz and D. C. Morrison, Chemical and biological properties of a protein-rich fraction of bacterial lipopolysaccharides. I. The in vitro murine lymphocyte response, J. Immunol. 119: 1790 (1977).
D. C. Morrison, M. E. Wilson, S. Raziuddin, S. J. Betz, B. J. Curry, Z. G. Oades, and P. Munkenbeck, The influence of lipid A-associated protein on endotoxin stimulation of non-lymphoid cells, in: “Microbiology,” D. Schlessinger, ed., American Society for Microbiology, Washington, D. C. (1980).
M. H. Ginsberg and D. C. Morrison, The selective binding of aggregated IgG to lipid A-rich bacterial lipopolysaccharides, J. Immunol. 120: 317 (1978).
K. Yokoyama, J. Mashimo, N. Kasai, T. Terao, and T. Osawa, Binding of bacterial lipopolysaccharide to histocompatibility-2-complex proteins of mouse lymphocytes, Hoppe Seyler’s Z. Physiol. Chem. 360: 587 (1979).
K. Kuus, A. R. Johnston, and R. J. Ulevitch, Enhancement of C3H/HeJ B-cell responsiveness to lipopolysaccharide (LPS) with Trypsin, Fed. Proc. (Abst.) 44: 974 (1985).
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Morrison, D.C., Wollenweber, HW., Vukajlovich, S.W., Goodman, S.A. (1986). Biochemical, Immunological and Functional Analysis of Lymphocytes from the LPS-Non-Responder C3H/HeJ Mouse. In: Szentivanyi, A., Friedman, H., Nowotny, A. (eds) Immunobiology and Immunopharmacology of Bacterial Endotoxins. University of South Florida International Biomedical Symposia Series, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2253-5_28
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