Abstract
Bacterial lipopolysaccharide (LPS) extracted from the cell walls of gram-negative bacteria has a number of effects on the cells of the immune system (see 21 for review). The effects of LPS on macrophages (Mø) and B lymphocytes are very prominent. Mø activated by LPS increase their phagocytic ability (20,21), pinocytosis (5,30), oxidative metabolism (15,25), synthesis of cellular proteins including lysosomal enzymes (19,20), secretion of collagenase (36) and arginase (4), tumor cytotoxicity (33), microbicidal activity (27), interleukin (IL) 1 (18), colony-stimulating factor, and interferon (21). When murine splenic B lymphocytes are stimulated by LPS, about one-third of the B lymphocytes initiates DNA synthesis (13), and subsequently, these activated B lymphocytes produce antibodies polyclonally (21). However, its effect on human peripheral B lymphocytes is not obvious (21,26,29), unless these cells are cultured for long periods of time (seven to nine days) with prescreened lots of fresh human serum (17). On the contrary, LPS has no obvious mitogenic effect on T lymphocytes, except on a very small percentage of cells (35), and it is incapable of initiating T lymphocytes to produce lymphokines. However, if T lymphocytes are activated by T cell stimulants, the T lymphocytes seem to be able to accept the stimulus of LPS. The combination of some kinds of phytomitogens with LPS can synergistically enhance the blastogenic responses of thymocytes in mice (7,24,32), spleen lymhocytes of rats (8) or peripheral blood T lymphocytes of humans (12,29) as measured by increase[3H]thymidine ([3H]TdR)uptakes.
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© 1986 Plenum Press, New York
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Nakano, M., Nitta, T. (1986). Synergistic Effect of Endotoxin with Concanavalin a on DNA Synthesis in Lymphocytes and the Role of Interleukins 1 and 2. In: Szentivanyi, A., Friedman, H., Nowotny, A. (eds) Immunobiology and Immunopharmacology of Bacterial Endotoxins. University of South Florida International Biomedical Symposia Series, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2253-5_27
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DOI: https://doi.org/10.1007/978-1-4613-2253-5_27
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