Expression of Cloned Muscle-Specific Genes in Transfected Cells and Transgenic Mice
The control of expression of muscle-specific genes introduced into myogenic cells and transgenic mice was studied. The expression of chimeric genes containing the 5′ regions and flanking DNA of the genes coding for rat skeletal muscle actin and myosin light chain 2 was greatly increased during differentiation of stably transfected myogenic clones and in transiently transfected myogenic cells. Likewise, the expression of the chicken skeletal muscle actin gene was developmentally regulated in several stably transfected rat myogenic clones, indicating that the mechanisms controlling muscle-specific gene expression have been conserved for at least 300 million years. In contrast, the expression of a fused mouse/human β-globin gene, and of a chimeric gene containing the 5′ region and flanking cDNA of the rat β-actin gene did not Increase during differentiation of transfected myogenic clones. The expression of the rat MLC2 gene was tissue-specific and developmentally regulated in transgenic mice.
KeywordsActin Gene Globin Gene Chimeric Gene Myogenic Cell Chloramphenicol Acetyl Transferase
Unable to display preview. Download preview PDF.
- Czosnek, H., Nudel, U., Shani, M., Barker, P.E., Pravtcheva, D., Ruddle, F.H. and Yaffe, D., 1982, The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2 and skeletal muscle actin are located on three different mouse chromosomes, EMBO J., 1: 1299.PubMedGoogle Scholar
- Czosnek, H., Nudel, U., Mayer, Y., Barker, P.E., Pravtcheva, D.D., Ruddle, F.H. and Yaffe, D., 1983, The genes coding for the cardiac muscle actin, the skeletal muscle actin and the cytoplasmic β-actin are located on three different mouse chromosomes. EMBO J., 11: 1977.Google Scholar
- de Saint Vincent, B.R., Delbruck, S., Eckhart, W., Meinkoth, J.,Vitto, L. and Wahl, G., 1981, The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker, Cell, 27: 267.Google Scholar
- Efstatiadis, A., Pasakony, J.W., Maniatis, T., Lawn, R.M., O’Connell, C., Spritz, R.A., DeRiel, J.K., Forget, B.G., Weissman, S.M., Slightom, J.L., Blechi, A.E., Smithies, O., Baralle, F.E., Schoulders, C.C. and Proudoot, N.J., 1980, The structure and evolution of the human β-globin gene family, Cell, 21: 653CrossRefGoogle Scholar
- Melloul, D., Aloni, B., Calvo, J., Yaffe, D. and Nudel, U., 1984, Developmentally regulated expression of chimeric genes containing muscle actin DNA sequences in transfected myogenic cells, EMBO J. 3:983,Google Scholar
- Nudel, U., Melloul, D., Aloni, B., Greenberg, D. and Yaffe, D., 1984b, Developmentally regulated expression of chimeric muscle genes transferred into myogenic cells, In, Molecular Biology of Development, E. Davidson and R. Firtel, eds., p647, Alan R. Liss, Inc., New York.Google Scholar
- Nudel, U., Melloul, D., Aloni, B., Greenberg, D. and Yaffe, D., 1985b, The promoter regions of two muscle-specific genes containing DNA sequences which confer tissue- and stage-specific expression of chimeric genes in transfected myogenic cells, In, Molecular Biology of Muscle Development, UCLA Symp. on Molecular and Cellular Biology, New Series, Vol. 29, C. Emerson, D.A. Fischman, B. Nadal-Ginard and M.A.Q. Siddiqui, eds., Alan R. Liss, Inc., New York.Google Scholar
- Shani, M. and Yaffe, D, 1985, Expression of rat skeletal muscle genes in transgenic mice, In, Molecular Biology of Muscle Development, UCLA Symp., Molecular and Cellular Biology, New Series, Vol. 29, Alan R. Liss, Inc., New York.Google Scholar
- Shani, M., Zevin-Sonkin, D., Saxel, O., Carmon, Y., Katcoff, D., Nudel, U. and Yaffe, D., 1981, The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of the corresponding mRNA sequences during myogenesis, Dev. Biol., 86: 483.PubMedCrossRefGoogle Scholar
- Vanderkerckhove, J. and Weber, K., 1979, The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation, Differentiation, 14: 124.Google Scholar