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Expression of Cloned Muscle-Specific Genes in Transfected Cells and Transgenic Mice

  • David Yaffe
  • Uri Nudel
  • Moshe Shani
  • Danielle Melloul
  • Batya Aloni
  • Yitzhak Mayer
  • David Greenberg

Summary

The control of expression of muscle-specific genes introduced into myogenic cells and transgenic mice was studied. The expression of chimeric genes containing the 5′ regions and flanking DNA of the genes coding for rat skeletal muscle actin and myosin light chain 2 was greatly increased during differentiation of stably transfected myogenic clones and in transiently transfected myogenic cells. Likewise, the expression of the chicken skeletal muscle actin gene was developmentally regulated in several stably transfected rat myogenic clones, indicating that the mechanisms controlling muscle-specific gene expression have been conserved for at least 300 million years. In contrast, the expression of a fused mouse/human β-globin gene, and of a chimeric gene containing the 5′ region and flanking cDNA of the rat β-actin gene did not Increase during differentiation of transfected myogenic clones. The expression of the rat MLC2 gene was tissue-specific and developmentally regulated in transgenic mice.

Keywords

Actin Gene Globin Gene Chimeric Gene Myogenic Cell Chloramphenicol Acetyl Transferase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Carmon, Y., Czosnek, H., Nudel, U., Shani, M. and Yaffe, D., 1982, DNAase I sensitivity of genes expressed during myogenesis. Nucl. Acids Res., 10: 3085PubMedCrossRefGoogle Scholar
  2. Chao, M.V., Mellon, P., Charnay, F., Maniatis, T. and Axel, R., 1983, The regulated expression of β-globin genes introduced into eryth- roleukemia cells, Cell, 32: 483.PubMedCrossRefGoogle Scholar
  3. Charnay, P., Treisman, R., Mellon, P., Chao, M., Axel, R. and Maniatis T., 1984, Differences in human α- and β-globin gene expression in mouse erythroleukemia cells: The role of intragenic sequences, Cell, 38: 251.PubMedCrossRefGoogle Scholar
  4. Czosnek, H., Nudel, U., Shani, M., Barker, P.E., Pravtcheva, D., Ruddle, F.H. and Yaffe, D., 1982, The genes coding for the muscle contractile proteins, myosin heavy chain, myosin light chain 2 and skeletal muscle actin are located on three different mouse chromosomes, EMBO J., 1: 1299.PubMedGoogle Scholar
  5. Czosnek, H., Nudel, U., Mayer, Y., Barker, P.E., Pravtcheva, D.D., Ruddle, F.H. and Yaffe, D., 1983, The genes coding for the cardiac muscle actin, the skeletal muscle actin and the cytoplasmic β-actin are located on three different mouse chromosomes. EMBO J., 11: 1977.Google Scholar
  6. de Saint Vincent, B.R., Delbruck, S., Eckhart, W., Meinkoth, J.,Vitto, L. and Wahl, G., 1981, The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker, Cell, 27: 267.Google Scholar
  7. Efstatiadis, A., Pasakony, J.W., Maniatis, T., Lawn, R.M., O’Connell, C., Spritz, R.A., DeRiel, J.K., Forget, B.G., Weissman, S.M., Slightom, J.L., Blechi, A.E., Smithies, O., Baralle, F.E., Schoulders, C.C. and Proudoot, N.J., 1980, The structure and evolution of the human β-globin gene family, Cell, 21: 653CrossRefGoogle Scholar
  8. Kurtz, D.T., 1981, Hormone inducibility of α2µ. -globulin genes in transfected mouse cells, Nature, 261: 629.CrossRefGoogle Scholar
  9. Lacy, E., Roberts, S., Evans, E.P., Burtenshaw, M.D. and Costantini, F.D., 1983, A foreign β-globin gene in transgenic mice: integration at abnormal chromosomal positions and expression in inappropriate tissues, Cell, 34: 343.PubMedCrossRefGoogle Scholar
  10. Mayer, Y., Czosnek, H., Zeelon, P.E., Yaffe, D. and Nudel, U., 1984, Expression of the gene coding for the skeletal muscle and cardiac actin in the heart, Nucl, Acids Res., 12: 1087.CrossRefGoogle Scholar
  11. Melloul, D., Aloni, B., Calvo, J., Yaffe, D. and Nudel, U., 1984, Developmentally regulated expression of chimeric genes containing muscle actin DNA sequences in transfected myogenic cells, EMBO J. 3:983,Google Scholar
  12. Nudel, U., Calvo, J.M., Shani, M. and Levy, Z., 1984a, The nucleotide sequence of rat myosin light chain 2 gene, Nucl. Acids Res., 12: 7175.PubMedCrossRefGoogle Scholar
  13. Nudel, U., Melloul, D., Aloni, B., Greenberg, D. and Yaffe, D., 1984b, Developmentally regulated expression of chimeric muscle genes transferred into myogenic cells, In, Molecular Biology of Development, E. Davidson and R. Firtel, eds., p647, Alan R. Liss, Inc., New York.Google Scholar
  14. Nudel, U., Greenberg, D., Ordahl, C.P., Saxel, O., Neuman, S. and Yaffe, D., 1985a, Developmentally regulated expression of a chicken muscle-specific gene in stably transfected rat myogenic cells, Proc. Natl. Acad. Sci. USA, 82: 3106.PubMedCrossRefGoogle Scholar
  15. Nudel, U., Melloul, D., Aloni, B., Greenberg, D. and Yaffe, D., 1985b, The promoter regions of two muscle-specific genes containing DNA sequences which confer tissue- and stage-specific expression of chimeric genes in transfected myogenic cells, In, Molecular Biology of Muscle Development, UCLA Symp. on Molecular and Cellular Biology, New Series, Vol. 29, C. Emerson, D.A. Fischman, B. Nadal-Ginard and M.A.Q. Siddiqui, eds., Alan R. Liss, Inc., New York.Google Scholar
  16. Shani, M., 1985, Tissue-specific expression of rat myosin light chain 2 gene in transgenic mice, Nature, 314: 283.PubMedCrossRefGoogle Scholar
  17. Shani, M. and Yaffe, D, 1985, Expression of rat skeletal muscle genes in transgenic mice, In, Molecular Biology of Muscle Development, UCLA Symp., Molecular and Cellular Biology, New Series, Vol. 29, Alan R. Liss, Inc., New York.Google Scholar
  18. Shani, M., Zevin-Sonkin, D., Saxel, O., Carmon, Y., Katcoff, D., Nudel, U. and Yaffe, D., 1981, The correlation between the synthesis of skeletal muscle actin, myosin heavy chain, and myosin light chain and the accumulation of the corresponding mRNA sequences during myogenesis, Dev. Biol., 86: 483.PubMedCrossRefGoogle Scholar
  19. Vanderkerckhove, J. and Weber, K., 1979, The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation, Differentiation, 14: 124.Google Scholar
  20. Walker, M.D., Edlund, T., Boulet, A.M. and Rutter, W.J., 1983, Cell-specific expression controlled by the 5’-flanking region of insulin and chymotrypsin genes, Nature, 306: 557.PubMedCrossRefGoogle Scholar
  21. Wright, S., Rosenthal, A., Flavell, R. and Grosveld, F., 1984, DNA sequences required for regulated experession of β-globin genes in murine erythroleukemia cells, Cell, 38: 265.PubMedCrossRefGoogle Scholar
  22. Yablonka, Z. and Yaffe, D., 1977, Synthesis of myosin light chains and accumulation of translatable mRNA coding for light chain-like polypeptides in differentiating muscle cultures, Differentiation, 8: 133.PubMedCrossRefGoogle Scholar
  23. Yaffe, D., Nudel, U., Mayer, Y. and Neuman, S., 1985, Highly conserved Sequences in the 3’-untranslated region of mRNAs coding for homologous proteins in distantly related species, Nucl. Acids Res., 13: 3723.PubMedCrossRefGoogle Scholar
  24. Yaffe, D. and Saxel, O., 1977, A myogenic cell line with altered serum requirements for differentiation, Differentiation, 7: 159.PubMedCrossRefGoogle Scholar
  25. Zakut, R., Shani, M., Givol, D., Neuman, S., Yaffe, D. and Nudel, U., 1982, Nucleotide sequence of the rat skeletal muscle actin gene, Nature, 298: 857.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • David Yaffe
    • 1
  • Uri Nudel
    • 1
  • Moshe Shani
    • 1
  • Danielle Melloul
    • 1
  • Batya Aloni
    • 1
  • Yitzhak Mayer
    • 1
  • David Greenberg
    • 1
  1. 1.Department of Cell BiologyThe Weizmann Institute of ScienceRehovotIsrael

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