Expression of Cloned Muscle-Specific Genes in Transfected Cells and Transgenic Mice
The control of expression of muscle-specific genes introduced into myogenic cells and transgenic mice was studied. The expression of chimeric genes containing the 5′ regions and flanking DNA of the genes coding for rat skeletal muscle actin and myosin light chain 2 was greatly increased during differentiation of stably transfected myogenic clones and in transiently transfected myogenic cells. Likewise, the expression of the chicken skeletal muscle actin gene was developmentally regulated in several stably transfected rat myogenic clones, indicating that the mechanisms controlling muscle-specific gene expression have been conserved for at least 300 million years. In contrast, the expression of a fused mouse/human β-globin gene, and of a chimeric gene containing the 5′ region and flanking cDNA of the rat β-actin gene did not Increase during differentiation of transfected myogenic clones. The expression of the rat MLC2 gene was tissue-specific and developmentally regulated in transgenic mice.
KeywordsUrea Polypeptide Acetyl Creatine Chloramphenicol
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