Skip to main content

Physarum Myosin Binds Ca2+: Results From Electrophoresis and Equilibrium Dialysis Experiments

  • Chapter
The Molecular Biology of Physarum polycephalum

Part of the book series: NATO ASI Series ((NSSA,volume 106))

  • 118 Accesses

Abstract

We wish to understand the molecular mechanism of calcium-regulated cytoplasmic movements in the Plasmodium of Physarum polycephalum. Early studies demonstrated that the contractile activity of the cytoplasm, presumably an expression of the actomyosin ATPase activity, is influenced by Ca2+ (Hatano, 1970; Matthews, 1977). Recently it has become evident that the state of polymerization and aggregation of the actin filaments may also be Ca2+-regulated (see Chapter 9).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Burgess, W. H., Jemiolo, D. K., and Kretsinger, R. H., 1980, Interaction of calcium and calmodulin in the presence of sodium dodecyl sulfate, Biochim. Biophys. Acta, 623: 257.

    PubMed  CAS  Google Scholar 

  • Hatano, S., 1970, Specific effect of Ca2+ on movement of plasmodial fragment obtained by caffeine treatment, Exp. Cell Res ., 61: 199.

    Article  PubMed  CAS  Google Scholar 

  • Holroyde, M. J., Potter, J. D., and Solaro, R. J., 1979, The calcium binding properties of phosphorylated and unphosphorylated cardiac and skeletal myosins, J. Biol. Chem ., 254: 6478.

    PubMed  CAS  Google Scholar 

  • Kessler, D., 1982, Plasmodial structure and motility, in: “Cell Biology of Physarum and Didymium”, Vol. I, H. C. Aldrich and J. W. Daniel, eds., Academic Press, New York.

    Google Scholar 

  • Kessler, D., and Dolberg, B. K., 1985, Physarum myosin binds calcium equilibrium dialysis results, J. Cell Biol., 101: 162a.

    Google Scholar 

  • Kessler, D., Eisenlohr, L. C., Lathwell, M. J., Huang, J., Taylor, H. C., Godfrey, S. D., and Spady, M. L., 1980. Physarum myosin light chain binds calcium, Cell Motil ., 1: 63.

    Article  PubMed  CAS  Google Scholar 

  • Kohama, K., 1981, Ca-dependent inhibitory factor for the myosin-actin-ATP interaction of Physarum myosin, J. Biochem ., 90: 1829.

    PubMed  CAS  Google Scholar 

  • Kohama, K., and Kendrick-Jones, J., 1982, Negative Ca2+-sensitivity of actin-activated Mg-ATPase activity of myosin from Physarum polycephalum, J. Muscle Res. Cell Motil ., 3: 491.

    Google Scholar 

  • Laemmli, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature, 227: 680.

    Article  PubMed  CAS  Google Scholar 

  • Maruyama, K., Mikawa, T., and Ebashi, S., 1984, Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis, J. Biochem ., 95: 511.

    PubMed  CAS  Google Scholar 

  • Matthews, L. M., Jr., 1977, Calcium ion regulation in caffeine derived microplasmodia of Physarum polycephalum, J. Cell Biol ., 72: 502.

    Article  PubMed  CAS  Google Scholar 

  • Nachmias, V. T., 1981a, Hybrids of Physarum myosin light chains and desensitized scallop myofibrils, J. Cell. Biol ., 90: 408.

    Article  PubMed  CAS  Google Scholar 

  • Nachmias, V. T., 1981b, Physarum myosin light chain one: A potential regulatory factor in cytoplasmic streaming, Protoplasma, 109: 13.

    Article  CAS  Google Scholar 

  • Nachmias, V. T., 1982, Purification of myosin from Physarum polycephalum, in: “Cell Biology of Physarum and Didymium”, Vol. II, H. C. Aldrich, and J. W. Daniel, eds., Academic Press, New York.

    Google Scholar 

  • Potter, J. D., Strang-Brown, P., Walker, P. L., and Iida, S., 1983, Ca2+ binding to calmodulin, Methods Enzymol ., 102: 135.

    Article  PubMed  CAS  Google Scholar 

  • Studier, F. W., 1973, Analysis of bacteriophage T7 early RNAs and proteins on slab gels, J. Mol. Biol ., 79: 237.

    Article  PubMed  CAS  Google Scholar 

  • Van Eldik, L. J., Piperno, G., and Watterson, D. M., 1980, Comparative biochemistry of calmodulins and calmodulin-like proteins, Ann. N.Y. Acad. Sci ., 356: 36.

    Article  PubMed  Google Scholar 

  • Weeds, AG., and Lowey, S., 1971, Substructure of myosin molecule. II. The light chains of myosin, J. Mol. Biol ., 61: 701.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1986 Plenum Press, New York

About this chapter

Cite this chapter

Kessler, D., Dolberg, B.K. (1986). Physarum Myosin Binds Ca2+: Results From Electrophoresis and Equilibrium Dialysis Experiments. In: Dove, W.F., Dee, J., Hatano, S., Haugli, F.B., Wohlfarth-Bottermann, KE. (eds) The Molecular Biology of Physarum polycephalum . NATO ASI Series, vol 106. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2203-0_11

Download citation

  • DOI: https://doi.org/10.1007/978-1-4613-2203-0_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9294-4

  • Online ISBN: 978-1-4613-2203-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics