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Amyloidosis pp 477-481 | Cite as

Amyloidogenicity and Subgroups of Human Lambda Bence Jones Proteins

  • Takashi Isobi
  • Hiroshi Tonoike
  • Fuyuki Kametani
  • Tomotaka Shinoda

Abstract

The nature of Bence Jones protein (BJP) NIG-77 (Kor) has been biochemically characterized. This BJP λ easily forms white and viscous precipitates in the cold in more than 8 mg/ml. This insolubility property is ascribed to one tryptic peptide involving residues 23–46, which corresponds with the first complementarity-determining regions (CDR) of the V-domain of BJP.

Complete sequence analysis has been carried out to see if there is any correlation between the primary structure and amyloidogenicity. On the basis of sequence homology of CDR as well as whole variable region, BJP NIG-77 has been classified in subsubgroup Vλ I-2 closely related to amyloidosis, in contrast to presumably non-amyloidogenic subsubgroup Vλ I-1 (1).

Keywords

Tryptic Peptide Versus Region Systemic Amyloidosis Crude Material Bence Jones Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Takashi Isobi
    • 1
  • Hiroshi Tonoike
    • 2
  • Fuyuki Kametani
    • 2
  • Tomotaka Shinoda
    • 1
  1. 1.Department of MedicineKobe University School of MedicineKobeJapan
  2. 2.Department of BiochemistryTokyo Metropolitan UniversityTokyoJapan

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