Amyloidogenicity and Subgroups of Human Lambda Bence Jones Proteins
The nature of Bence Jones protein (BJP) NIG-77 (Kor) has been biochemically characterized. This BJP λ easily forms white and viscous precipitates in the cold in more than 8 mg/ml. This insolubility property is ascribed to one tryptic peptide involving residues 23–46, which corresponds with the first complementarity-determining regions (CDR) of the V-domain of BJP.
Complete sequence analysis has been carried out to see if there is any correlation between the primary structure and amyloidogenicity. On the basis of sequence homology of CDR as well as whole variable region, BJP NIG-77 has been classified in subsubgroup Vλ I-2 closely related to amyloidosis, in contrast to presumably non-amyloidogenic subsubgroup Vλ I-1 (1).
KeywordsHPLC Carbohydrate Electrophoresis Fractionation Trypsin
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