Advertisement

Amyloidosis pp 245-251 | Cite as

In Vivo Degradation of Protein SAA to Protein AA and Incorporation in Amyloid Fibrils

  • A. Husebekk
  • B. Skogen
  • G. Husby
  • G. Marhaug

Abstract

To groups of mice, A and B, were injected intraperitoneally with LPS in order to induce amyloidosis. Group A got human HDL-SAA complexes intravenously in addition to LPS. The mice were sacrificed. The liver, spleen, and kidneys from both group A and B contained moderate amounts of amyloid examined by polarization microscopy after Congo red staining. Antiserum to human AA/SAA was tested and showed no cross reactivity with mouse SAA or AA.

Isolated amyloid fibrils from the mice in group A contained human protein AA. This was shown by double immunodiffusion, immunoblot, and ELISA techniques. In contrast, mice in group B had only mouse amyloid fibrils. The experiments provided solid evidence that SAA is the precursor for amyloid fibril protein AA.

Keywords

Amyloid Deposit Amyloid Fibril Amyloid Protein Filter Strip Antigenic Reactivity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. B. Pepys and M. L. Baltz, Adv. Immunol., 34: 141 (1983).PubMedCrossRefGoogle Scholar
  2. 2.
    G. Husby and J. B. Natvig, J. Clin. Invest., 53: 1054 (1974).PubMedCrossRefGoogle Scholar
  3. 3.
    G. Husby, K. Sletten, T. E. Michaelsen, and J. B. Natvig, Scand. J. Immunol., 1: 393 (1972).PubMedCrossRefGoogle Scholar
  4. 4.
    M. Pras, M. Schubert, D. Zucker-Franklin, A. Rimon, and E. C. Franklin, J. Clin. Invest., 47: 924 (1968).PubMedCrossRefGoogle Scholar
  5. 5.
    C. J. Rosenthal and E. C. Franklin, J. Clin. Invest., 53: 1054 (1974).CrossRefGoogle Scholar
  6. 6.
    R. F. Anders, J. B. Natvig, K. Sletten, G. Husby, and K. Nordstoga, J. Immunol., 118: 229 (1977).PubMedGoogle Scholar
  7. 7.
    E. P. Benditt and N. Eriksen, Proc. Natl. Acad. Sci. USA, 74: 4025 (1977).PubMedCrossRefGoogle Scholar
  8. 8.
    B. Skogen, A. L. Børresen, J. B. Natvig, K. Berg, and T. Michaelsen, Scand. J. Immunol., 10: 39 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    R. F. Anders, J. B. Natvig, and G. Husby, J. Exp. Med., 143: 678 (1976).PubMedCrossRefGoogle Scholar
  10. 10.
    R. Kisilevski, L. Boudreau, and B. Foster, Lab. Invest., 48: 60 (1983).Google Scholar
  11. 11.
    G. Lavie, D. Zucker-Franklin, and E. C. Franklin, J. Exp. Med., 148: 1020 (1978).PubMedCrossRefGoogle Scholar
  12. 12.
    K. Sletten, G. Marhaug, and G. Husby, Hoppe-SeylerTs Z. Physiol. Chem., 364: 1039 (1983).CrossRefGoogle Scholar
  13. 13.
    B. Skogen and J. B. Matvig, Scand. J. Immunol., 14: 389 (1981).PubMedCrossRefGoogle Scholar
  14. 14.
    B. Skogen, L. Thorsteinsson, and J. B. Natvig, Scand. J. Immunol., 11: 533 (1980).PubMedCrossRefGoogle Scholar
  15. 15.
    R. J. Havel, H. A. Eder, and J. H. Bragdon, J. Clin. Invest., 34: 1345 (1955).PubMedCrossRefGoogle Scholar
  16. 16.
    R. F. Anders, J. B. Natvig, T. E. Michaelsen, and G. Husby, Scand. J. Immunol., 4: 397 (1975).PubMedCrossRefGoogle Scholar
  17. 17.
    G. Marhaug, G. Gaudernack, B. Bogen, and G. Husby, Clin. Exp. Immunol 50: 390 (1982).PubMedGoogle Scholar
  18. 18.
    R. T. Swank and K. D. Munkres, Analyt. Biochem., 39: 462 (1971).PubMedCrossRefGoogle Scholar
  19. 19.
    H. Towbin, T. Staehelin, and J. Gordon, Proc. Natl. Acad. Sci. USA, 76: 4350 (1979).CrossRefGoogle Scholar
  20. 20.
    G. Marhaug, Scand. J. Immunol., 18: 329 (1983).PubMedCrossRefGoogle Scholar
  21. 21.
    G. Marhaug, K. Sletten, and G. Husby, in: Amyloidosis E.A.R.S. ( C. R Tribe and P. A. Bacon, eds.), John Wright and Sons Limited, Bristol-London-Boston (1983), pp. 15–18.Google Scholar
  22. 22.
    K. Sletten and G. Husby, Eur. J. Biochem., 41: 117 (1974).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • A. Husebekk
    • 1
  • B. Skogen
    • 1
  • G. Husby
    • 1
  • G. Marhaug
    • 1
  1. 1.Department of Rheumatology Institute of Clinical MedicineUniversity of TomsøTomsøNorway

Personalised recommendations