In Vivo Degradation of Protein SAA to Protein AA and Incorporation in Amyloid Fibrils
To groups of mice, A and B, were injected intraperitoneally with LPS in order to induce amyloidosis. Group A got human HDL-SAA complexes intravenously in addition to LPS. The mice were sacrificed. The liver, spleen, and kidneys from both group A and B contained moderate amounts of amyloid examined by polarization microscopy after Congo red staining. Antiserum to human AA/SAA was tested and showed no cross reactivity with mouse SAA or AA.
Isolated amyloid fibrils from the mice in group A contained human protein AA. This was shown by double immunodiffusion, immunoblot, and ELISA techniques. In contrast, mice in group B had only mouse amyloid fibrils. The experiments provided solid evidence that SAA is the precursor for amyloid fibril protein AA.
KeywordsFiltration Albumin EDTA Agarose Electrophoresis
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