Abstract
The requirement for Ca2+ in the response of the isolated barley aleurone layer to gibberellic acid (GA) was first reported by Chrispeels and Varner (1967). These workers showed that when Ca2+ was omitted from the incubation medium the synthesis of α-amylase in response to GA was reduced by 50% in imbibed half-grains and by 80% in isolated aleurone layers. Jacobsen et al. (1970) showed that two isoenzymes of α-amylase were produced when aleurone layers were incubated in GA and another two when Ca2+ was added. The a-amylase isoenzymes synthesized in response to GA have been referred to as group A: they belong to the same antigenic group, have isoelectric points (pIs) around 5 and are sensitive to Hg salts and N-ethylmaleimide. α-Amylases produced on addition of Ca2+ are referred to as group-B isoenzymes: they belong to a different antigenic group, have pis around 6.0, and are stable to Hg and N-ethylmaleimide (Jacobsen et al., 1970; Jacobsen and Higgins, 1982).
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© 1986 Plenum Press, New York
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Jones, R.L., Deikman, J., Melroy, D. (1986). Role of Ca2+ in the Regulation of α-Amylase Synthesis and Secretion in Barley Aleurone. In: Trewavas, A.J. (eds) Molecular and Cellular Aspects of Calcium in Plant Development. NATO ASI Series, vol 104. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2177-4_7
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DOI: https://doi.org/10.1007/978-1-4613-2177-4_7
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