Phosphorylation of Membrane-Located Proteins of Soybean: In Vitro Response of Purified Plasma Membranes to Auxin and Calcium

  • Robert L. Varnold
  • D. James Morré
  • Anna Stina Sandelius
Part of the NATO ASI Series book series (volume 104)

Abstract

Isolated membranes of soybean incorpated 32P from γ-[32P]ATP in vitro. The incorporation was rapid and labeling was achieved in 10 s or less. When displayed on 10% sodium dodecylsulfate-polyacrylamide gels, several labeled protein bands were revealed (Morré et al., 1984). Incorporation into protein bands with apparent molecular weights of 45,000 and 50,000 as well as into total material insoluble in trichloroacetic acid was observed in the presence of auxin (2,4-dichlorophenoxyacetic acid = 2,4-D). Also, incorporation into a low molecular weight constituent was stimulated 2- to 3-fold by auxin in some experiments. The activity may be the result of protein kinases of the C-type since stimulation also was given by diglyceride plus calcium (Morré et al., 1984), constituents known to augment C-type kinases in other systems (Nishizuka, 1984).

Keywords

Sucrose Polypeptide Dextran Inositol Polyethyleneglycol 

References

  1. Kjellbom, P., and C. Larsson, 1984, Preparation and polypeptide composition of chlorophyll-free plasma membranes from leaves of light- grown spinach and barley, Physiol. Plantarum 62: 501.CrossRefGoogle Scholar
  2. Morré, D. J., Morré, J. T., and Varnold, R. L., 1984, Phosphorylation of membrane-located proteins of soybean in vitro and response to auxin. Plant Physiol. 75: 265.CrossRefGoogle Scholar
  3. Nishizuka, Y., 1984, Turnover of inositol phospholipids and signal transduction. Science 225: 1265.CrossRefGoogle Scholar
  4. Varnold, R. L., and Morré, D. J., 1985, Phosphorylation of membrane-located proteins of soybean hypocotyl. Inhibition by calcium in the presence of 2,4-dichlorophenoxyacetic acid. Bot. Gaz.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Robert L. Varnold
    • 1
    • 2
  • D. James Morré
    • 1
    • 2
  • Anna Stina Sandelius
    • 1
    • 2
  1. 1.Department of Medicinal ChemistryPurdue UniversityWest LafayetteUSA
  2. 2.Department of Biological SciencesPurdue UniversityWest LafayetteUSA

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