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Calcium Uptake and Exchange in Barley Aleurone Layers and Protoplasts During Ca-Stimulated Amylase Secretion

  • Douglas S. Bush
  • Russell L. Jones
Part of the NATO ASI Series book series (volume 104)

Abstract

Extracellular Ca2+ regulates α-amylase secretion in isolated barley aleurone layers. At high Ca2+ concentrations (1–10 mM) the secretion of isozymes of α-amylase with high isoelectric points (high pI) is greatly stimulated. This effect is rapidly reversed by withdrawing Ca2+ and is specific for Ca2+. Extracellular Ca2+ may exert its effect either directly on the plasma membrane or by altering the Ca concentration of some compartment inside the cell. We have determined the influence of extracellular Ca2+ on intracellular Ca pools by measuring Ca uptake and exchange in aleurone layers and by direct measurement of cytoplasmic Ca2+ activity in aleurone protoplasts.

Keywords

Calcium Uptake Gibberellic Acid Constant Fraction Aleurone Layer Small Pool 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Douglas S. Bush
    • 1
  • Russell L. Jones
    • 1
  1. 1.Department of BotanyUniversity of CaliforniaBerkeleyUSA

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