The Concentration of Calmodulin Alters During Early Stages of Cell Development in the root apex of Pisum Sativum

  • E. E. F. Allan
  • A. J. Trewavas
Part of the NATO ASI Series book series (volume 104)

Abstract

Quantitative changes in calmodulin were investigated during early stages of cell development in the root apex of Pisum sativum. Concentrations were estimated in two ways: highly purified samples of calmodulin extracted from serial developmental sections were estimated by NAD kinase activating ability, and whole cell extracts of individual tissue areas from serial sections were examined by two-dimensional polyacrylamide gel electrophoresis. Extraction conditions were designed to solubilise membrane-bound calmodulin, and to minimise interference from calmodulin-binding proteins which appeared to reduce the extractability of calmodulin from pea seedling tissues.

Keywords

Electrophoresis Polypeptide Polyacrylamide 

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • E. E. F. Allan
    • 1
  • A. J. Trewavas
    • 1
  1. 1.Botany DepartmentUniversity of EdinburghEdinburghUK

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