Ca2+-Dependence of Callose Synthesis and the Role of Polyamines in the Activation of 1,3-β-Glucan Synthase by Ca2+
During recent investigations we tried to establish a convenient system in which several reactions could be simultaneously induced which are regarded to be of importance in the resistance of plants to pathogens. We used suspension-cultured soybean cells with the aim to affect as many cells as possible at the same time in order to have a low background of undisturbed cells, and to allow accurate time studies for biochemical work. As an “elicitor” we selected the natural polycation chitosan (= deacetylated chitin) as experimental results with basic polyamino acids (e.g. Poly-L-Lys1) suggested that the primary interaction of such molecules with the cell or its membrane might be relatively nonspecific. This allowed to avoid pragmatically the consideration whether or not group-specific receptors are involved in plant/pathogen interactions.
KeywordsAniline Blue Soybean Cell Callose Synthesis Callose Formation Free Unsaturated Fatty Acid
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