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Receptor Cycling in Pituitary Cells: Biological Consequences of Inhibition of Receptor-Mediated Endocytosis

  • Patricia M. Hinkle
  • Jane Halpern
  • Patricia A. Kinsella
  • Risa A. Freedman

Abstract

The anterior pituitary gland of mammalian species secretes six major trophic hormones: adrenocorticotropic hormone, two gonadotropins, thyrotrophin, prolactin and growth hormone. Synthesis and secretion of these pituitary hormones is regulated in part by hypothalamic factors which reach the pituitary gland by a portal system and which act upon specific target cells to stimulate or inhibit hormone production. In addition, pituitary function is regulated by circulating hormones including steroids and peptides.

Keywords

Epidermal Growth Factor Pituitary Cell Wheat Germ Agglutinin Prolactin Secretion Epidermal Growth Factor Binding 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aizawa, T. and Hinkle, P.M., 1985, TRH rapidly stimulates a biphasic secretion of PRL and GH in GH4C1 rat pituitary tumor cells, Endocrinology, 116: 73–8.Google Scholar
  2. Goldstein, J.L., Anderson, R.G.W. and Brown, M.S., 1979, Coated pits, coated vesicles, and receptor-mediated endocytosis, Nature, 279: 679–68.Google Scholar
  3. Gourdji, D., Tougard, C. and Tixier-Yidal, A., 1981, Clonal prolactin stains as a tool in neuroendocrinology, in: “Frontiers in Neuroendocrinology,” vol. 7, W.F. Ganong and L. Martini, eds., pp. 317–35, Raven Press, New York.Google Scholar
  4. Haigler, H.T., Maxfield, F.R., Willingham, M.C. and Pastan, I.H., 1980, Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells, J Biol. Chem., 255:1239–124.Google Scholar
  5. Hal pern, J. and Hinkle, P.M., 1981, Direct visualization of receptors for thyrotropin-releasing hormone with a fluorescein labeled analog, Proc. Natl. Acad. Sci., 78:587–59.Google Scholar
  6. Hal pern, J. and Hinkle, P.M., 1983, Binding and internalization of epidermal growth factor by rat pituitary tumor cells, Mol. Cell Endocrinol., 33:183–19.Google Scholar
  7. Hinkle, P.M., 1984, Interaction of peptide hormones with rat pituitary tumor cells in culture, in: “Secretory Tumors of the Pituitary Gland,” P. Mcl. Black, N.T. Zervos, E.C. Ridgway and J.B. Martin, eds., pp. 25–4, Raven Press, New York.Google Scholar
  8. Hinkle, P.M. and Kinsella, P.A., 1982, Rapid temperature dependent transformation of the thyrotropin-releasing hormone receptor complex in rat pituitary tumor cells, J. Biol. Chem., 257:5462–547.Google Scholar
  9. Hinkle, P.M. and Tashjian, A.H., Jr., 1973, Receptors for thyrotropin releasing hormone in prolactin producing rat pituitary cells in culture, J. Biol. Chem, 148:6180–618.Google Scholar
  10. Hinkle, P.M. and Tashjian, A.H., Jr., 1975a, Thyrotropin-releasing hormone regulates the number of its own receptors in the GH3 strain of pituitary cells in culture, Biochem., 14: 3845–385.Google Scholar
  11. Hinkle, P.M. and Tashjian, A.H., Jr., 1975b, Degradation of thyrotropin-releasing hormone by the GH3 strain of pituitary cells in culture, Endocrinology, 97: 324–33.Google Scholar
  12. Kaplan, J., 1981, Polypeptide-binding membrane receptors: analysis and classification, Science, 212: 14–2.CrossRefGoogle Scholar
  13. King, A.C. and Cuatrecasas, P., 1981, Peptide hormone induced receptor mobility, aggregation and internalization, New Engl. J. Med., 305:77–8.Google Scholar
  14. Pastan, I.H. and Willingham, M.C., 1981, Receptor-mediated endocytosis of hormones in cultured cells, Ann. Rev. Physiol., 43:239–25.Google Scholar
  15. Presky, D.H. and Schonbrunn, A., 1983, Receptor-bound somatostatin (SRIF) and epidermal growth factor (EGF) are processed differently by GH4C1 pituitary cells. Program of the 65th Annual Meeting of the Endocrine Society, A 564, p. 221.Google Scholar
  16. Seglen, P.O, 1983, Inhibitors of lysosomal function, Methods Enzymol., 96: 737–76.Google Scholar
  17. Tashjian, A.H., Jr., 1979, Clonal strains of hormone-producing pituitary cells. Methods Enzymol., 58: 527–53.Google Scholar
  18. Tashjian, A.H., Jr., Yasumura, Y., Sato, G.H, Parker, M.L., 1968, Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone, Endocrinology, 82: 342–35.Google Scholar
  19. Tixier-Vidal, A. and Gourdji, D., 1981, Mechanism of action of synthetic hypothalamic peptides on anterior pituitary cells, Physiol. Rev., 61:974–101.Google Scholar
  20. Webb, J.L., 1966, “Enzyme and Metabolic Inhibitors,” Chapter 6, pp. 595–64, Academic Press, New York.Google Scholar
  21. Westendorf, J.M. and Schonbrunn, A., 1983, Characterization of bombesin receptors in a rat pituitary cell line, J. Biol. Chem., 258:7527–753.Google Scholar
  22. Willingham, M.C. and Pastan, I., 1980, The receptosome: an intermediate organelle of receptor-mediated endocytosis in cultured fibroblasts, Cell, 21: 67–7.Google Scholar

Copyright information

© Plenum Press, New York 1986

Authors and Affiliations

  • Patricia M. Hinkle
    • 1
  • Jane Halpern
    • 1
  • Patricia A. Kinsella
    • 1
  • Risa A. Freedman
    • 1
  1. 1.Department of Pharmacology and the Cancer CenterUniversity of Rochester, School of Medicine and DentistryRochesterUSA

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