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Cryopreservation of Mammalian Eggs and Embryos

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Manipulation of Mammalian Development

Part of the book series: Developmental Biology ((DEBO,volume 4))

Abstract

In the decade since reliable methods of freezing mouse embryos were first described (Whittingham et al., 1972; Wilmut, 1972a) methods have been developed for freezing the embryos of a number of other species. Frozen embryos have been used in the treatment of human infertility, in farm animal breeding, and in biological research. During this period, the procedures have also been simplified.

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References

  • Bank, H., and Maurer, R. R., 1974, Survival of frozen rabbit embryos, Exp. Cell Res. 89: 188–196.

    Article  PubMed  CAS  Google Scholar 

  • Bilton, R. J., 1980, Preservation of embryos of the large domestic species, Proc. 9th Int. Cong. Anim. Reprod. A.I. 2: 245–253.

    Google Scholar 

  • Blears, D. J., and Danyluk, S. S., 1968, Proton wide-line nuclear magnetic resonance spectra of hydrated proteins, Biochim. Biophys. Acta 154: 17–27.

    PubMed  CAS  Google Scholar 

  • Bouyssou, B., and Chupin, D., 1981, Two step freezing of cattle blastocysts with dimethysulphoxide (DMSO) of glycerol, Theriogenology 17: 159–167.

    Article  Google Scholar 

  • Butler, W. J., and Roberts, T. K., 1975, Effects of some Phosphatidyl compounds on boar spermatozoa following cold shock or slow cooling, J. Reprod. Fertil. 43: 183–187.

    Article  PubMed  CAS  Google Scholar 

  • Chang, M. C., and Walton, A., 1940, The effect of low temperature and acclimatization on the respiratory activity and survival of ram spermatozoa, Proc. R. Soc., Lond. B 129: 517–527.

    Article  Google Scholar 

  • Dick, D. A. T., 1966, Cell Water, Butterworths, Washington, D.C.

    Google Scholar 

  • Edidin, M., and Petit, V. A., 1977, The effect of temperature on the lateral diffusion of plasma membrane proteins, in: The Freezing of Mammalian Embryos (K. Elliott and J. Whelan, eds.), pp. 155–165, CIBA Symposium 52, Elsevier, Amsterdam.

    Google Scholar 

  • Elford, B. C., 1970a, Diffusion and distribution of diethyl sulphoxide in the isolated Guinea-pig taenia coli, J. Physiol. (Lond.) 209: 187–208.

    CAS  Google Scholar 

  • Elford, B. C., 1970b, Non-solvent water in muscle, Nature (Lond.) 227: 282–283.

    Article  CAS  Google Scholar 

  • Farrant, J., 1977, Water transport and cell survival in cryobiological procedures, Philos. Trans. R. Soc. Lond. B 278: 191–205.

    Article  CAS  Google Scholar 

  • Farrant, J., 1980, General observations on cell preservations, in: Low Temperature Preservation in Medicine and Biology ( M. J. Ashwood-Smith and J. Farrant, eds.), Pitman Medical Tunbridge, Wells.

    Google Scholar 

  • Farrant, J., and Morris, G. J., 1973, Thermal shock and dilution shock as the causes of freezing injury, Cryobiology 10: 134–140.

    Article  PubMed  CAS  Google Scholar 

  • Farrant, J., Lee, H., and Walter, C. A., 1978, Effects of interactions between cooling and rewarming conditions on survival of cells, in: The Freezing of Mammalian Embryos (K. Elliott and J. Whelan, eds.), pp. 49–63, CIBA Symposium 52, Elsevier, Amsterdam.

    Google Scholar 

  • Frank, H. S., 1965, The structure of water, Fed. Proc. (Suppl.) 15: S1–S11.

    Google Scholar 

  • Glenister, P. H., and Lyon, M. F., 1981, Experience of banking mutant genes, in: Frozen Storage of Laboratory Animals ( G. H. Zeilmaker, ed.), pp. 157–163, Gustav Fischer, Stuttgart.

    Google Scholar 

  • Hazlewood, C. F., Nichols, B. L., and Chamberlain, N. F., 1969, Evidence for the existence of a minimum of two phases of ordered water in skeletal muscle, Nature (Lond.) 222: 747–750.

    Article  CAS  Google Scholar 

  • Hechter, D., 1965, Role of water structure in the molecular organisation of cell membranes, Fed. Proc. (Suppl.) 15: S91–S102.

    Google Scholar 

  • Kasai, M., Niwa, K., and Iritani, A., 1981, Effects of various cryoprotective agents on the survival of unfrozen and frozen mouse embryos, J. Reprod. Fertil. 63: 175–180.

    Article  PubMed  CAS  Google Scholar 

  • Klotz, I. M., 1965, Role of water structure in macromolecules, Fed. Proc. (Suppl.) 15: S24–S33.

    Google Scholar 

  • Leibo, S. P., 1977, Fundamental cryobiology of mouse ova and embryos, (K. Elliott and J. Whelan, eds.), pp. 69–72. Elsevier, Amsterdam.

    Google Scholar 

  • Leibo, S. P., and Mazur, P., 1978, Methods for the preservation of mammalian embryos by freezing, in: Methods in Mammalian Reproduction ( J. C. Daniel, Jr., ed.), pp. 179–201, Academic Press, New York.

    Google Scholar 

  • Leibo, S. P., Mazur, P., and Jackowski, S. C., 1974, Factors affecting survival of mouse embryos during freezing and thawing, Exp. Cell Res. 89: 79–88.

    Article  PubMed  CAS  Google Scholar 

  • Leibo, S. P., West, A. W., Perry, B., and Mills, A. C., 1982a, A one-step method for direct non-surgical transfer of frozen thawed bovine embryos. II. Applied Studies, Proc. Soc. Cryobiol, p. 79 (abst.).

    Google Scholar 

  • Leibo, S. P., West, A. W., and Perry, B. 1982b, A one-step method for direct non-surgical transfer of frozen thawed bovine embryos. 1. Basic studies, Proc. Soc. Cryobiol., p. 78 (abst.).

    Google Scholar 

  • Lovelock, J. E., 1953, The haemolysis of human red blood-cells by freezing and thawing, Biochim. Biophys. Acta 10: 414–426.

    Article  PubMed  CAS  Google Scholar 

  • Lovelock, J. E., 1954, The protective action of neutral solutes against haemolysis by freezing and thawing, Biochem. J. 56: 265–270.

    PubMed  CAS  Google Scholar 

  • Luyet, B., and Keane, J., 1955, A critical temperature range apparently characterised by sensitivity of bull semen to high freezing velocity, Biodynamics 7: 281–292.

    CAS  Google Scholar 

  • Massip, A., Van Der Zawlmen, P., Hanzen, C., and Ectors, F., 1982, Fast freezing of cow embryos in French strains with an automatic program, Theriogenology 18: 325–332.

    Article  PubMed  CAS  Google Scholar 

  • Maurer, R. R., and Haseman, J. K., 1976, Freezing morula stage rabbit embryos, Biol. Reprod. 14: 251–263.

    Article  Google Scholar 

  • Mazur, P., 1970, The freezing of biological systems, Cryobiology 168: 939–949.

    CAS  Google Scholar 

  • Mazur, P., Farrant, J., Leibo, S. P., and Chu, E. H. Y., 1969, Survival of hamster tissue culture cells after freezing and thawing, Cryobiology 6: 1–9.

    Article  PubMed  CAS  Google Scholar 

  • Mazur, P., Leibo, S. P., Farrant, J., Chu, E. H. Y., Hanna, M. G., and Smith, L. H., 1970, Interactions of cooling rate, warming rate and protective additive on the survival of frozen mammalian cells, in: The Frozen Cell ( G. E. W. Wolstenholme and M. O’Connor, eds.), pp. 69–85, Churchill Livingstone, London.

    Google Scholar 

  • Mazur, P., Rail, W. F., and Rigopoulos, N., 1981, Relative contributions of the fraction of unfrozen water and of salt concentrations to the survival of slowly frozen human erythrocytes, Biophys. J. 36: 653–675.

    Article  PubMed  CAS  Google Scholar 

  • Miller, R. H., and Mazur, P., 1976, Survival of frozen-thawed human red cells as a function of cooling and warming velocities, Cryobiology 13: 404–414.

    Article  PubMed  CAS  Google Scholar 

  • Miyamoto, H., and Ishibashi, T., 1977, Survival of frozen-thawed mouse and rat embryos in the presence of ethylene glycol, J. Reprod. Fertil. 50: 373–375.

    Article  PubMed  CAS  Google Scholar 

  • Miyamoto, H., and Ishibashi, T., 1978, The protective action of glycols against freezing damage of mouse and rat embryos, J. Reprod. Fertil. 54: 427–432.

    Article  PubMed  CAS  Google Scholar 

  • Miyamoto, H., and Ishibashi, T., 1981, Survival of mouse embryos after freezing and thawing in the presence of erythritol, J. Exp. Zool. 216: 337–340.

    Article  PubMed  CAS  Google Scholar 

  • Miyamoto, H., and Ishibashi, T., 1983a, Survival of mouse embryos frozen-thawed slowly or rapidly in the presence of various cryoprotectants, J. Exp. Zool. 226: 123–127.

    Article  PubMed  CAS  Google Scholar 

  • Miyamoto, H., and Ishibashi, T., 1983b, Solid CO2 freezing of mouse embryos, J. Reprod. Fertil. 67: 107–111.

    Article  PubMed  CAS  Google Scholar 

  • Moore, N. W., and Bilton, R. J., 1976, Storage, culture and transfer of embryos of domestic animals, Proc. 8th Int. Corigr. Anim. Reprod. A. I. Krakow 3: 306–308.

    Google Scholar 

  • Moore, N. W., and Bilton, R. J., 1977, Frozen storage of embryos of farm animals: Progress and implications, in: The Freezing of Mammalian Embryos ( K. Elliott and J. Whelan, eds.), pp. 203–211, Elsevier, Amsterdam.

    Google Scholar 

  • Morris, G. J., and Farrant, J., 1973, Effects of cooling rate on thermal shock hemolysis, Cryobiology 10: 119–125.

    Article  PubMed  CAS  Google Scholar 

  • Nieman, H., Sacher, B., Schilling, E., and Smidt, D., 1982, Improvement of survival rates of bovine blastocysts with sucrose for glycerol dilution after a fast freezing and thawing method, Theriogenology 17: 102.

    Article  Google Scholar 

  • Parkes, A. S., and Smith, A. U., 1953, Regeneration of rat ovarian tissue grafted after exposure to low temperatures, Proc. R. Soc. Lond. B 140: 455–470.

    Article  PubMed  CAS  Google Scholar 

  • Parrott, D. M. V., 1960, The fertility of mice with orthotopic ovarian grafts derived from frozen tissue, J. Reprod. Fertil. 1: 230–241.

    Article  Google Scholar 

  • Polge, C., 1957, Low-temperature storage of mammalian spermatozoa, Proc. R. Soc. B 147: 498–508.

    Article  CAS  Google Scholar 

  • Polge, C., 1977, The freezing of mammalian embryos: Perspectives and possibilities, in: The Freezing of Mammalian Embryos (K. Elliott and J. Whelan, eds., pp. 3–13, CIBA Symposium 52, Elsevier, Amsterdam.

    Google Scholar 

  • Polge, C., Smith, A. U., and Parkes, A. S., 1949, Revival of spermatozoa after vitrification and dehydration at low temperatures, Nature (Lond.) 164: 666.

    Article  CAS  Google Scholar 

  • Polge, C., Wilmut, I., and Rowson, L. E. A., 1974, The low temperature preservation of cow, sheep and pig embryos, Cryobiology 11: 560.

    Article  Google Scholar 

  • Rail, W. F., and Fahy, G. M., 1985, Ice-free cryopreservation of mouse embryos at -196° V by vitrification, Nature (Lond.) 313: 573–574.

    Article  Google Scholar 

  • Rail, W. F., and Polge, C. 1984, Effect of warming rate on mouse embryos frozen and thawed in glycerol, J. Reprod. Fertil. 70: 285–292.

    Article  Google Scholar 

  • Rail, W. F., Reid, D. S., and Farrant, J., 1980, Innocuous biological freezing during warming, Nature (Lond.) 286: 511–514.

    Article  Google Scholar 

  • Rail, W. F., Mazur, P., and McGrath, J. J., 1983, Depression of ice-nucleation temperature of rapidly cooled mouse embryos by glycerol and dimethyl sulphoxide, Biophys, J. 41: 1–12.

    Article  Google Scholar 

  • Rail, W. F., Czlonkowska, M., Barton, S. C., and Polge, C., 1984, Cryoprotection of Day 4 mouse embryos by methanol, J. Reprod. Fertil. 70: 293–300.

    Article  Google Scholar 

  • Rapatz, G., Sullivan, J. J., and Luyet, B., 1968, Preservation of erythrocytes in blood containing various cryoprotective agents, frozen at various rates and brought to a give feral temperature, Cryobiology 5: 18–25.

    Article  PubMed  CAS  Google Scholar 

  • Renard, J. P., Heyman, J., and Ozil, J. P., 1982, Congélation de l’embryon bovin. Une nouvelle méthode de décongélation pour le transfert cervical d’embryons conditionnés une seule fois en paillettes, Ann. Med. Vet. 126: 23–32.

    Google Scholar 

  • Rowson, L. E. A., and Moor, R. M., 1966, Embryo transfer in the sheep: The significance of synchronizing oestrus in the donor and recipient animal, J. Reprod. Fertil. 11: 207–212.

    Article  PubMed  CAS  Google Scholar 

  • Rowson, L. E. A., Moor, R. M., and Lawson, R. A. S., 1969, Fertility following egg transfer in the cow; effect of method, medium and synchronisation of oestrus, J. Reprod. Fertil. 18: 517–523.

    Article  PubMed  CAS  Google Scholar 

  • Schneider, U., and Maurer, R. R., 1983, Factors affecting survival of frozen-thawed mouse embryos, Biol. Reprod. 29: 121–128.

    Article  PubMed  CAS  Google Scholar 

  • Sherman, J. K., and Lin, T. P., 1958, Effect of glycerol and low temperature on survival of unfertilised mouse eggs, Nature (Lond.) 170: 374–375.

    Google Scholar 

  • Tait, M. J., and Franks, F., 1971, Water in biological systems, Nature (Lond.) 230: 91–94.

    Article  CAS  Google Scholar 

  • Trounson, A. D., and Mohr, L., 1983, Human pregnancy following cryopreservation, thawing and transfer of an eight-cell embryo, Nature (Lond). 305: 707–709.

    Article  CAS  Google Scholar 

  • Trounson, A. D., Willadsen, S. M., Rowson, L. E. A., and Newcomb, R., 1976, The storage of cow eggs at room temperature and at low temperatures, J. Reprod. Fertil. 46: 173–178.

    Article  PubMed  CAS  Google Scholar 

  • Trounson, A. D., Shea, B. F., Ollis, G. W., and Jacobson, M. E., 1978, Frozen storage and transfer of bovine embryos, J. Anim. Sci. 47: 677–681.

    PubMed  CAS  Google Scholar 

  • Tsunoda, Y., Soma, T., and Sugie, T., 1981, Frozen storage of rabbit and hamster embryos, in: Frozen Storage of Laboratory Animals ( G. H. Zeilmaker, ed.), pp. 129–139, Gustav Fischer, Stuttgart.

    Google Scholar 

  • Walton, A., 1957, Cold shock of spermatozoa, Proc. R. Soc. B 147: 508–509.

    Article  CAS  Google Scholar 

  • Whittingham, D. G. 1974, The viability of frozen-thawed mouse blastocysts, J. Reprod. Fertil. 37: 159–162.

    Article  PubMed  CAS  Google Scholar 

  • Whittingham, D. G., 1975, Survival of rat embryos after freezing and thawing, J. Reprod. Fertil. 43: 515–518.

    Article  Google Scholar 

  • Whittingham, D. G., 1981a, Sensitivity of mouse embryos to the rate of thawing, in: Frozen Storage and Laboratory Animals ( G. H. Zeilmaker, ed.), pp. 21–32, Gustav Fischer, Stuttgart.

    Google Scholar 

  • Whittingham, D. G., 1981b, Discussion, in: Frozen Storage of Laboratory Animals ( G. H. Zeilmaker, ed.), p. 58, Gustav Fischer, Stuttgart.

    Google Scholar 

  • Whittingham, D. G., and Adams, C. E. 1976, Low temperature preservation of rabbit embryos, J. Reprod. Fertil. 47: 269–274.

    Article  PubMed  CAS  Google Scholar 

  • Whittingham, D. G., Leibo, S. P., and Mazur, P., 1972, Survival of mouse embryos frozen to −196° and −269°C, Science 178: 411–414.

    Article  PubMed  CAS  Google Scholar 

  • Whittingham, D. G., Wood, M., Farrant, J., Lee, H., and Halsley, J. A., 1979, Survival of frozen mouse embryos after rapid thawing from −196°C, J. Reprod. Fertil. 56: 11–21.

    Article  PubMed  CAS  Google Scholar 

  • Willadsen, S. M., 1977, Factors affecting the survival of sheep embryos during deep-freezing and thawing, in: The Freezing of Mammalian Embryos (K. Elliott and J. Whelan, eds.), pp. 175–189. CIBA Symposium 52, Elsevier, Amsterdam.

    Google Scholar 

  • Willadsen, S. M., 1980, Deep freezing of embryos in the large domestic species, Proc. 9th Int. Cong. Anim. Reprod. A.I. 2: 255–261.

    Google Scholar 

  • Willadsen, S. M., Polge, C., Rowson, L. E. A., and Moor, R. M., 1976a, Deep freezing of sheep embryos, J. Reprod. Fertil. 46: 151–154.

    Article  PubMed  CAS  Google Scholar 

  • Willadsen, S. M., Trounson, A. O., Rowson, L. E. A., and Newcomb, R., 1976, Low temperature preservation of cow eggs, in: Egg Transfer in Cattle ( L. E. A. Rowson, ed.), pp. 117–124, Commission of the European Communities, Luxembourg.

    Google Scholar 

  • Willadsen, S. M., Polge, C., and Rowson, L. E. A., 1978, In vitro storage of cattle embryos, in: Control of Reproduction in the Cow ( J. R. Sreenan, ed.), pp. 427–436, Martinus Nijhoff, The Hague.

    Google Scholar 

  • Wilmut, I., 1972a, The effect of cooling rate, warming rate, cryoprotective agent and stage of development on survival of mouse embryos during freezing and thawing, Life Sci. 2 (2): 1071–1079.

    Article  Google Scholar 

  • Wilmut, I., 1972b, The low temperature preservation of mammalian embryos, J. Reprod. Fertil. 31: 513–514.

    Article  PubMed  CAS  Google Scholar 

  • Wilmut, I., and Rowson, L. E. A., 1973, Experiments on the low-temperature preservation of cow embryos, Vet. Ree. 92: 686–690.

    Article  CAS  Google Scholar 

  • Wilmut, I., Polge, C., and Rowson, L. E. A., 1975, The effect on cow embryos of cooling to 20, 0 and −196°C, J. Reprod. Fertil. 45: 409–411.

    Article  PubMed  CAS  Google Scholar 

  • Wood, M. J., and Farrant, J., 1980, Preservation of mouse embryos by two-step freezing, Cryobiology 17: 178–180.

    Article  PubMed  CAS  Google Scholar 

  • Wood, M. J., and Whittingham, D. G., 1981, Low temperature storage of rat embryos, in: Frozen Storage of Laboratory Animals ( G. H. Zeilmaker, ed.), pp. 119–127, Gustav Fischer, Stuttgart.

    Google Scholar 

  • Yokoyama, M., Wakasuki, N., and Nomura, T., (1981), An attempt to store inbred mouse strains, in: Frozen Storage of Laboratory Animals ( G. H. Zeilmaker, ed.), pp. 113–117, Gustav Fischer, Stuttgart.

    Google Scholar 

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© 1986 Plenum Press, New York

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Wilmut, I. (1986). Cryopreservation of Mammalian Eggs and Embryos. In: Gwatkin, R.B.L. (eds) Manipulation of Mammalian Development. Developmental Biology, vol 4. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2143-9_7

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  • DOI: https://doi.org/10.1007/978-1-4613-2143-9_7

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