Regulation of Gene Expression from the Polyoma Late Promoter

  • F. G. Kern
  • P. Delli-Bovi
  • S. Pellegrini
  • C. Basilico
Part of the Developments in Molecular Virology book series (DMVI, volume 9)

Abstract

An experimental system has been developed in which foreign coding sequences for easily assayed genes or selectable markers are placed under the control of the polyoma late promoter. The use of this system has allowed the elucidation of the cisacting elements that control expression from this promoter and the demonstration of transactivation of this promoter by the viral early proteins. In addition to these forms of transcriptional control, the results also suggest that late transcription, both in polyoma transformed cells and early in the course of a lytic infection, may also be regulated at a posttranscriptional level.

Keywords

Agar Attenuation Recombination Agarose Sarcoma 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Tooze, J. DNA tumor virus: Molecular Biology of Tumor Viruses, part 2. Cold Spring Harbor Laboratory, Cold Spring Harbor N.Y., 1980.Google Scholar
  2. 2.
    Franke, B. and Eckhart, W. Virology 55:1 27–135, 1973.Google Scholar
  3. 3.
    Gaudray, P., Tyndall, C., Kamen, R. and Cuzin, F. Nucleic Acids Res. 9:5697–5710, 1981.PubMedCrossRefGoogle Scholar
  4. 4.
    Dilworth, S.M., Cowie, A., Kamen, R. and Griffin, B.E. Proc. Natl. Acad. Sci. U.S.A. 81:1941–1945, 1984.PubMedCrossRefGoogle Scholar
  5. 5.
    Pomerantz, B.J., Mueller, C.R. and Hassell, J.A. J. Virol. 47:600–610, 1983.PubMedGoogle Scholar
  6. 6.
    Fenton, R.G. and Basilico, C. Virology 121:384–392, 1982.PubMedCrossRefGoogle Scholar
  7. 7.
    Farmerie, W.G. and Folk, W.R. Proc. Natl. Acad. Sci. U.S.A. 81:6919–6923, 1984.PubMedCrossRefGoogle Scholar
  8. 8.
    Icheson, N.H. Proc. Natl J Acad. Sci. U.S.A. 75:4754–4758, 1978.CrossRefGoogle Scholar
  9. 9.
    Treisman, R. Nucleic Acids Res. 8:4867–4888, 1980.PubMedCrossRefGoogle Scholar
  10. 10.
    Soeda, E., Arrand, A., Smolar, N., Walsh, J. and Griffin, B., 1980. Nature 283:445–453.PubMedCrossRefGoogle Scholar
  11. 11.
    Legon, S. J. Mol. Biol. 134:219–240, 1979.CrossRefGoogle Scholar
  12. 12.
    Adami, G.R. and Carmichael, G.G. J. Virol. 58:417–425, 1986.PubMedGoogle Scholar
  13. 13.
    Luthman, H., Nilsson, M.G. and Magnusson, G. J. Mol. Biol. 161:533–550, 1982.CrossRefGoogle Scholar
  14. 14.
    Katinka, M. and Yaniv, M. J. Virol. 47:244–248, 1983.PubMedGoogle Scholar
  15. 15.
    Cowie, A. and Kamen, R. J. Virol. 52:750–760, 1984.PubMedGoogle Scholar
  16. 16.
    de Villiers, J. and Schaffner, W. Nucleic Acids Res. 9:6251–6264, 1981.PubMedCrossRefGoogle Scholar
  17. 17.
    Tyndall, C., La Mantia, G., Thacker, C.M., Favaloro, J. and Kamen, R. Nucleic Acids Res. 9:6231–6250, 1981.PubMedCrossRefGoogle Scholar
  18. 18.
    Banerji, J., Rusconi, S. and Schaffner, W. Cell 27:299–308, 1981.PubMedCrossRefGoogle Scholar
  19. 19.
    Jat, P., Novak, U., Cowie, A., Tyndall, C. and Kamen, R. Mol. Cell. Biol. 2:737–751, 1982.PubMedGoogle Scholar
  20. 20.
    Cowie, A., Tyndall, C. and Kamen, R. Nucleic Acids Res. 9:6305–6322, 1981.PubMedCrossRefGoogle Scholar
  21. 21.
    de Villiers, J., Schaffner, W., Tyndall, C., Lupton, S. and Kamen, R. Nature 312:242–246, 1984.PubMedCrossRefGoogle Scholar
  22. 22.
    Basilico, C. Pharmac. Ther. 26:235–272, 1985.CrossRefGoogle Scholar
  23. 23.
    Treisman, R., Novak, V., Favaloro, J. and Kamen, R. Nature 292:595–600, 1981.PubMedCrossRefGoogle Scholar
  24. 24.
    Pillegrini, S., Dailey, L. and Basilico, C. Cell 3:943–949, 1984.CrossRefGoogle Scholar
  25. 25.
    Kern, F.G. and Basilico, C. Mol. Cell. Biol. 5:797–807, 1985.PubMedGoogle Scholar
  26. 26.
    Kern, F.G., Dailey, L. and Basilico, C. Mol. Cell. Biol. 5:2070–2079, 1985.PubMedGoogle Scholar
  27. 27.
    Kern, F.G., Pellegrini, S. and Basilico, C. In: Cancer Cells, vol. 4, DNA Tumor Viruses: Control of Gene Expression and Replication. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., in press.Google Scholar
  28. 28.
    Kern, F.G., Pellegrini, S., Cowie, A. and Basilico, C. J. Virol., in press.Google Scholar
  29. 29.
    Colantuoni, V., Dailey, L. and Basilico, C. Proc. Natl. Acad. Sci. U.S.A. 77:3850–3854.Google Scholar
  30. 30.
    Frischauf, A.M., Lehrach, H., Poutska, A. and Murray, N. J. Mol. Biol. 170:824–842.Google Scholar
  31. 31.
    Basilico, C., Fenton, R.G. and Delia Valle, G. In: Expression of Differentiated Functions in Cancer Cells (Ed. R.F. Revoltella), Raven Press, New York, 1982, pp. 323–335.Google Scholar
  32. 32.
    Colberre-Garapin, F., Horodniceanu, F., Kourilsky, P. and Garapin, A.C. J. Mol. Biol. 150:1–14, 1981.CrossRefGoogle Scholar
  33. 33.
    Zenke, M., Grundstrom, T., Matthes, H., Winzerith, M., Schatz, C., Wildeman, A. and Chambon, P. EMBO J. 5:387–397, 1986.PubMedGoogle Scholar
  34. 34.
    Herbomel, P., Bourachot, B. and Yaniv, M. Cell 39:653–662, 1984.PubMedCrossRefGoogle Scholar
  35. 35.
    Maione, R., Passananti, C., De Simone, V., Delli-Bovi, P., Augusti-Tocco, G. and Amati, P. EMBO J. 4:3215–3221, 1985.PubMedGoogle Scholar
  36. 36.
    Weiher, H., Konig, M. and Gruss, P. Science 219:626–631, 1983.PubMedCrossRefGoogle Scholar
  37. 37.
    Veldman, G.M., Lupton, S. and Kamen, R. Mol. Cell. Biol. 5:649–658, 1985.PubMedGoogle Scholar
  38. 38.
    Hearing, P. and Shenk, T. Cell 33:695–703, 1983.PubMedCrossRefGoogle Scholar
  39. 39.
    Gorman, CM., Moffat, L.F. and Howard, B.H. Mol. Cell. Biol. 2:1044–1051, 1982.PubMedGoogle Scholar
  40. 40.
    Wong, G.G., Witch, J.S., Temple, P.A., Wilkins, K.M., Leary, A.C., Luxenberg, D.P., Jones, S.S., Brown E.L., Kay, R.M., Orr, E.C., Shoemaker, C, Golde, D.W., Kaufman, R.J., Hewick, R.M., Wang, E.A. and Clark, S.C. Science 228:810–815, 1985.PubMedCrossRefGoogle Scholar
  41. 41.
    Lanoix, J., Tseng, R.W. and Acheson, N.H. J. Virol. 58:733–742, 1986.PubMedGoogle Scholar
  42. 42.
    Izant, J.G. and Weintraub, H. Cell 36:1007–1015, 1984.PubMedCrossRefGoogle Scholar
  43. 43.
    Kamen, R., Jat, P., Treisman, R. and Favaloro, J. J. Mol. Biol. 159:189–224, 1982.CrossRefGoogle Scholar
  44. 44.
    Fenton, R.G. and Basilico, C. Proc. Natl. Acad. Sci. U.S.A. 79:7142–7146, 1982.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • F. G. Kern
    • 1
  • P. Delli-Bovi
    • 1
  • S. Pellegrini
    • 1
  • C. Basilico
    • 1
  1. 1.Department of PathologyNew York University School of MedicineNew YorkUSA

Personalised recommendations