Abstract
Some features of the 80S nucleoprotein extracted from the nuclei of SV40-infected cells have been explored. A region of the SV40 genome including the origin of replication and about 400 base pairs of adjacent sequence is contained in an altered chromatin structure, rendering it hypersensitive to endonuclease digestion and accessible to exonuclease digestion once an endonucleolytic cut has been introduced. DNA sequences within this region are responsible for the nuclease-sensitive configuration. At least two genetic elements, each of which can generate a hypersensitive site, have been mapped to the region between nucleotide positions 37 and 287. Insertion of segments of DNA at position 37/38 does not disrupt the hypersensitive site but moves it away from the origin of replication. Insertion of 90 base pairs or more causes the origin to lie outside the hypersensitive site and the resulting genome replicates inefficiently. This result suggests that efficient functioning of the viral replication origin may depend on location within a hypersensitive site in the chromatin. The same may be true for viral promoters although the genetic evidence is incomplete.
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Scott, W.A. and Wigmore, D.J. Cell 15:1511–1518, 1978.
Waldeck, W., Fohring, B., Chowdhury, K., Gruss, P. and Sauer, G. Proc. Natl. Acad. Sci. USA 75:5964–5968, 1978.
Varshavsky, A.J., Sundin, O. and Bohn, M. Cell 16:453–466, 1979.
Elgin, S.C.R. Cell 27:413–415, 1981.
Cartwright, I.L., Abmayr, S.M., Fleischmann, G., Lowenhaupt, K., Elgin, S.C.R., Keene, M.A. and Howard, G.C. CRC Crit. Rev. Biochem. 13:1–86, 1982.
Weisbrod, S. Nature (London) 297:289–295, 1982.
Shelton, E.R., Wassarman, P.M. and DePamphilis, M.L. J. Biol. Chem. 255:771–782, 1980.
Griffith, J.D. Science 187: 1201–1203, 1975.
Moyne, G., Freeman, R., Saragosti, S. and Yaniv, M. J. Mol. Biol. 149:735–744, 1981.
Garber, E.A., Seidman, M.M. and Levine, A.J. Virology 90:305–316, 1978.
Fernandez-Munoz, R., Coca-Prados, M. and Hsu, M.-T. J. Virol. 29:612–623, 1979.
Baumgartner, K., Kuhn, C. and Fanning, E. Virology 96:54–63, 1979.
Jakobovitz, E.B. and Aloni, Y. Virology 102:107–118, 1980.
Hartmann, J.P. and Scott, W.A. J. Virol. 46:1034–1038, 1983.
Weintraub, H. and Groudine, M. Science 193:848–856, 1976.
Buchman, A.R., Burnett, L. and Berg, P. In: DNA Tumor Viruses: Molecular Biology of Tumor Viruses (2nd ed.) Part 2. (Ed. J. Tooze), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1980, pp. 799–829.
Saragosti, S., Cereghini, S. and Yaniv, M. J. Mol. Biol. 160:133–146, 1982.
Scott, W.A., Walter, CF. and Cryer, B.L. Mol. Cell. Biol. 4:604–610, 1984.
Sundin, O. and Varshavsky, A. J. Mol. Biol. 132:535–546, 1979.
Lake, R.S., Barban, S. and Salzman, M.P. Biochem. Biophys. Res. Commun. 54:640–647, 1973.
Brady, J.N., Winston, V.D. and Consigli, R.A. J. Virol. 23:717–724, 1977.
Christiansen, G., Landers, T., Griffith, J. and Berg, P. J. Virol. 21:1079–1084, 1977.
Moyne, G., Harper, F., Saragosti, S. and Yaniv, M. Cell 30:123–130, 1982.
Hartmann, J.P. and Scott, W.A. J. Virol. 37:908–915, 1981.
Riley, D. and Weintraub, H. Cell 13:281–293, 1978.
Riley, D.E. Biochemistry 19:2977–2992, 1980.
Kunzler, P. and Stein, A. Biochemistry 22:1783–1789, 1983.
Scott, W.A., Wigmore, D.J. and Eaton, R.W. Miami Winter Symposia 16:83–98, 1979.
Wigmore, D.J., Eaton, R.W. and Scott, W.A. Virology 104:462–473, 1980.
Shenk, T. Cell 13:791–798, 1978.
Innis, J.W. and Scott, W.A. Mol. Cell. Biol. 4:1499–1507, 1984.
Gerard, R.D., Montelone, B.A., Walter, C.F., Innis, J.W. and Scott, W.A. Mol. Cell. Biol. 5:52–58, 1985.
Gerard, R.D., Woodworth-Gutai, M. and Scott, W.A. Mol. Cell. Biol. 2:782–788, 1982.
Innis, J.W. and Scott, W.A. Mol. Cell. Biol. 3:2203–2210, 1983.
Jongstra, J., Reudelhuber, T.L., Oudet, P., Benoist, C., Chae, C.-B., Jeltsch, J.-M., Mathis, D.J. and Chambon, P. Nature (London) 307:708–714, 1984.
Fromm, M. and Berg, P. Mol. Cell. Biol. 3:991–999, 1983.
Choder, M., Bratosin, S. and Aloni, Y. EMB0 J. 3:2929–2936, 1984.
Brady, J.N., Radonovich, M., Lavialle, C. and Salzman, N.P. J. Virol. 39:603–611, 1981.
Kondoleon, S.K., Robinson, G.W. and Hallick, L.M. Virology 129:261–273, 1983.
Ambrose, C., Blasquez, V. and Bina, M. Proc. Natl. Acad. Sci. USA 83:3287–3291, 1986.
Bergsma, D.J., Olive, D.M., Hartzell, S.W. and Subramanian, K.N. Proc. Natl. Acad. Sci. USA 79:381–385, 1982.
Weintraub, H. Cell 32:1191–1203, 1983.
Saragosti, S., Moyne, G. and Yaniv, M. Cell 20:65–73, 1980.
Jakobovits, E.B., Bratosin, S. and Aloni, Y. Nature (London) 285:263–265, 1980.
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© 1987 Martinus Nijhoff Publishers
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Scott, W.A. (1987). SV40 Chromatin Structure. In: Aloni, Y. (eds) Molecular Aspects of Papovaviruses. Developments in Molecular Virology, vol 9. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2087-6_10
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DOI: https://doi.org/10.1007/978-1-4613-2087-6_10
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