Immunocharacterization of the Nuclear Acceptor Sites for the Avian Oviduct Progesterone Receptor

  • A. Goldberger
  • M. Horton
  • T. C. Spelsberg


It is well known that steroid hormones, certain vitamins and sterols, enter target cells and bind to specific protein receptors in the cytoplasm or nucleus (1–4). This binding is saturable, high affinity, and steroid specific. Once bound by the steroid, the receptor undergoes chemical/conformation change, termed “activation,” which enables the receptor (as a complex with the steroid) to bind to nuclear acceptor sites on the nuclear chromatin (5,6) or nuclear matrix (7,8). It is generally accepted that the binding of the steroid receptor complex to these nuclear acceptor sites is a prerequisite for the steroid regulation of gene transcription and possibly post-transcriptional events.


Ascites Fluid Acceptor Site Chromosomal Protein Nuclear Binding Acceptor Protein 
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  1. 1.
    Thrall, C, Webster, R.A. and Spelsberg, T.C. In: The Cell Nucleus, Vol. VI, Part 1, (Ed. H. Busch), Academic Press, New York, 1978, pp. 461–529.Google Scholar
  2. 2.
    Spelsberg, T.C, Littlefield, B.A., Seelke, R., Martin-Dani, G., Toyoda, H., Boyd-Leinen, P., Thrall, C. and Kon, O.L. Recent Prog. Hormone Res. 39:463–517, 1983.Google Scholar
  3. 3.
    Moore, J.T., Norvitch, M.D., Wieben, E.D. and Veneziale, C.M. J. Biol. Chem. 259:14750–14756, 1984.PubMedGoogle Scholar
  4. 4.
    Jensen, E.V. and DeSombre, E.R. Ann. Rev. Biochem. 41:203–230, 1972.PubMedCrossRefGoogle Scholar
  5. 5.
    Spelsberg, T.C., Knowler, J., Boyd, P.A., Thrall, C.L. and Martin-Dani, G. J. Steroid Biochem. 11:373–388, 1979.PubMedCrossRefGoogle Scholar
  6. 6.
    Spelsberg, T.C. In: Biochemical Actions of Hormones, (Ed. G. Litwack), Academic Press, Vol. 9, 1982, pp. 141–204.Google Scholar
  7. 7.
    Barrack, E.R. and Coffey, D.S. J. Biol. Chem. 255:7265–7275, 1980.PubMedGoogle Scholar
  8. 8.
    Barrack, E.R. and Coffey, D.S. Recent Prog. Hormone Res. 38:133–195, 1982.Google Scholar
  9. 9.
    Martin-Dani, G. and Spelsberg, T.C. Biochemistry 24:6988–6997, 1985.CrossRefGoogle Scholar
  10. 10.
    Ruh, T.S. and Spelsberg, T.C. Biochem. J. 210:905–912, 1982.Google Scholar
  11. 11.
    Ruh, T.S., Ross, Jr., P., Wood, D.M. and Keene, J.L. Biochem. J. 200:133–142, 1981.PubMedGoogle Scholar
  12. 12.
    Ross, Jr. P. and Ruh, T.S. Biochim. Biophys. Acta 782:18–25, 1984.PubMedGoogle Scholar
  13. 13.
    Ruh, M.F., Singh, R.K., Bellone, C.J. and Ruh, T.S. Biochim. Biophys. Acta 844:24–33, 1985.PubMedCrossRefGoogle Scholar
  14. 14.
    Lopez, A., Burgos, J. and Ventanas, J. Internal J. Biochem. 17:1207–1211, 1985.CrossRefGoogle Scholar
  15. 15.
    Spelsberg, T.C, Gosse, B., Littlefield, B., Toyoda, H. and Seelke, R. Biochemistry 23:5103–5112, 1984.PubMedCrossRefGoogle Scholar
  16. 16.
    Cobb, A. and Leavitt, W.W. (abstract). 67th Annual Meeting of the Endocrine Society, June 19–21 (Baltimore, MD), pp. 83, No. 330.Google Scholar
  17. 17.
    Perry, B.N. and Lopez, A. Biochem. J. 176:873–883, 1978.PubMedGoogle Scholar
  18. 18.
    Klyzsejko-Stefanowicz, L., Chui, J.F., Tsai, Y.H. and Hnilica, L.S. Natl. Acad. Sci. USA 73:1954–1958, 1976.CrossRefGoogle Scholar
  19. 19.
    Hamana, K. and Iwai, K. J. Biochem. (Tokyo) 83:279–286, 1978.Google Scholar
  20. 20.
    Payvar, F., Wrange, O., Carlstedt-Duke, J., Okret, S., Gustafsson, J.A. and Yamamato, K. Proc. Natl. Acad. Sci. USA 78:6628–6632, 1981.PubMedCrossRefGoogle Scholar
  21. 21.
    Chandler, V.L., Maler, B.A. and Yamamoto, K.R. Cell 33:489–499, 1983.PubMedCrossRefGoogle Scholar
  22. 22.
    Payvar, F., DeFranco, D., Firestone, G.L., Edgar, B., Wrange, O., Okret, S., Gustafsson, J.A. and Yamamoto, K.R. Cell 35:381–392.Google Scholar
  23. 23.
    Lee, F., Mulligan, R., Berg P. and Ringold, G. Nature 294:228–232, 1981.PubMedCrossRefGoogle Scholar
  24. 24.
    Compton, J.G., Schrader, W.T. and O’Malley, B.W. Biochem. Biophys. Res. Commun. 105:96–104, 1982.PubMedCrossRefGoogle Scholar
  25. 25.
    Dean, D.C, Knoll, B.J., Riser, M.E. and O’Malley, B.W. Nature 305:551–554, 1983.PubMedCrossRefGoogle Scholar
  26. 26.
    Bailley, A., Atger, M., Atger, P., Cerbon, M.A., Alizon, M., Vu Hai, M.T., Logeat, F. and Milgrom, E. J. Biol. Chem. 258:10384–10389, 1983.Google Scholar
  27. 27.
    Mulvihill, E.R., LePennec, J.P. and Chambon, P. Cell 28:621–632, 1982.PubMedCrossRefGoogle Scholar
  28. 28.
    Miller, P.A., Ostrowski, M.C, Hager, G.L. and Simons, Jr., S.S. Biochemistry 23:6883–6889, 1984.PubMedCrossRefGoogle Scholar
  29. 29.
    deAhe, D., Janich, S., Scheidert, C, Renkawitz, R., Schutz, G. and Beato, M. Nature 313:706–709, 1985.CrossRefGoogle Scholar
  30. 30.
    Jost, J-P., Seldram, M., Geiser, M. Proc. Natl. Acad. Sci. USA 81:429–433, 1984.PubMedCrossRefGoogle Scholar
  31. 31.
    Goldberger, A., Littlefield, B.A., Katzmann, J. and Spelsberg, T.C Endocrinology 118:2235–2261, 1985.CrossRefGoogle Scholar
  32. 32.
    Spelsberg, T.C. Biochemistry 22:13–21, 1983.PubMedCrossRefGoogle Scholar
  33. 33.
    Boyd, P.A. and Spelsberg, T.C. Biochemistry 18:3685–3690, 1979.PubMedCrossRefGoogle Scholar
  34. 34.
    Boyd-Leinen, P., Gosse, B., Martin-Dani, G. and Spelsberg, T.C. J. Biol. Chem. 259:2411–2421, 1984.PubMedGoogle Scholar
  35. 35.
    Puri, R.K,, Grandies, P., Dougherty, J.J. and Toft, D.O. J. Biol. Chem. 257:10831–10837, 1982.PubMedGoogle Scholar
  36. 36.
    Dougherty, J.J., Puri, R.K. and Toft, D.O. J. Biol. Chem. 259:8004–8009, 1984.PubMedGoogle Scholar
  37. 37.
    Hora, J., Gosse, B., Rasmussen, K. and Spelsberg, T.C. Endocrinology 119:1118–1125, 1986.PubMedCrossRefGoogle Scholar
  38. 38.
    Boyd-Leinen, P.A., Fournier, D. and Spelsberg, T.C. Endocrinology 111:30–36, 1982.PubMedCrossRefGoogle Scholar
  39. 39.
    Thrall, T.C. and Spelsberg, T.C. Biochemistry 19:4130–4138, 1980.PubMedCrossRefGoogle Scholar
  40. 40.
    Littlefield, B.A. and Spelsberg, T.C. Endocrinology 117:412–424, 1984.CrossRefGoogle Scholar
  41. 41.
    Hora, J., Horton, M.J., Toft, D.O. and Spelsberg, T.C. Proc. Natl. Acad. Sci. USA, In press, 1986.Google Scholar
  42. 42.
    Toyoda, H., Seelke, R., Littlefield, B.A. and Spelsberg, T.C. Proc. Natl. Acad. Sci. USA 82:4722–4726, 1985.PubMedCrossRefGoogle Scholar
  43. 43.
    Spelsberg, T.C, Pikier, G.M. and Webster, R.A. Science 194:197–199, 1976.PubMedCrossRefGoogle Scholar
  44. 44.
    Kon, O.L. and Spelsberg, T.C. Endocrinology 111:1925–1936, 1982.PubMedCrossRefGoogle Scholar
  45. 45.
    Singh, R.K., Ruh, M.F. and Ruh, T.S. Biochem. Biophys. Acta 800:33–40, 1984.PubMedGoogle Scholar
  46. 46.
    Chuknyiska, R.S., Haji, M., Foote, R.H. and Roth, G.S. Endocrinology 115:836–838, 1984.PubMedCrossRefGoogle Scholar
  47. 47.
    Spelsberg, T.C., Reinhart, G.D. and Barham, S. Analytical Biochem. 143:237–248, 1984.CrossRefGoogle Scholar
  48. 48.
    Pikier, G.M., Webster, R.A. and Spelsberg, T.C. Biochem. J. 156:399–408, 1976.Google Scholar
  49. 49.
    Greene, G.L., Closs, L.E., Fleming, H., DeSombre, E.R. and Jensen, E.V. Proc. Natl. Acad. Sci. USA 74:3681–3685, 1977.PubMedCrossRefGoogle Scholar
  50. 50.
    Webster, R., Pikier, G. and Spelsberg, T. Biochem. J. 156:409–419, 1976.PubMedGoogle Scholar
  51. 51.
    Spelsberg, T., Webster, R. and Pikier, G. Nature. 262:65–67, 1976.PubMedCrossRefGoogle Scholar
  52. 52.
    Spelsberg, T.C. Biochem. J. 156:391–398, 1976.PubMedGoogle Scholar
  53. 53.
    Hewish, D.R. and Burgoyne, L.A. Biochem. Biophys. Res. Comm. 52:475–481, 1973PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1987

Authors and Affiliations

  • A. Goldberger
    • 1
  • M. Horton
    • 1
  • T. C. Spelsberg
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyMayo Clinic and Mayo Graduate School of MedicineRochesterUSA

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