Microvascularization of the alimentary canal as studied by scanning electron microscopy of corrosion casts

  • Aiji Ohtsuka
  • Osamu Ohtani
  • Takuro Murakami
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 4)


Scanning electron microscopy (SEM) of corrosion casts allows a clearer three-dimensional view of fine vascular architecture than conventional light or transmission electron microscopy of sectioned or injected tissue specimens (1–8). Positional relations of each vessel to the tissue elements cannot be examined in the cast samples, since the cast itself is observed in isolation from the surrounding tissues. However, SEM of vascular corrosion casts has already shown itself to be valuable and useful in the study of microvascular architecture in various organs and tissues, including the gastrointestinal canal (9–24).


Capillary Network Lymphoid Follicle Fundic Gland Capillary Plexus Vascular Corrosion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Murakami T: Application of the scanning electron microscope to the study of the fine distribution of the blood vessels. Arch Histol Jpn 32: 445–454, 1971.PubMedGoogle Scholar
  2. 2.
    Nowell JA, Lohse CL: Injection replication of the microvasculature for SEM. In: Scanning Electron Microscopy/1974-I, O Johari, I Corvin (eds), Chicago: IIT Research Institute, p 267–274, 1974.Google Scholar
  3. 3.
    Murakami, T: Methyl methacrylate injection replica method. In: Principles and Techniques of Scanning Electron Microscopy, vol. 6. MA Hayat (ed), New York: Van Nostrand Reinhold Co., p 159–169, 1978.Google Scholar
  4. 4.
    Gannon, BJ: Vascular casting. In: Principles and Techniques of Scanning Electron Microscopy, vol 6. MA Hayat (ed), New York: Van Nostrand Reinhold Co., p 170–193, 1978.Google Scholar
  5. 5.
    Frasca JM, Carter HW, Schaffer WA: An improved latex injection media for replicating the pulmonary microvasculature. In: Scanning Electron Microscopy/1978/II. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 485–489, 1978.Google Scholar
  6. 6.
    Hodde KC, Nowell JA: SEM of microcorrosion casts. In: Scanning Electron Microscopy/1980/11. RP Becker, O Johari (eds), Chicago: AMF O’Hare, SEM Inc, p 88–106, 1980.Google Scholar
  7. 7.
    Murakami T, Ohtani O, Ohtsuka A, Kikuta A: Injection replication and scanning electron microscopy of blood vessels. In: Biomedical Research Applications of Scanning Electron Microscopy, Vol 3. GM Hodges, KE Carr (eds), London: Academic Press, p 1–30, 1983.Google Scholar
  8. 8.
    Lametschwandtner A, Lametschwandtner U, Weiger T: Scanning electron microscopy of vascular corrosion casts - technique and applications. In: Scanning Electron Microscopy/1984/II. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 663–695, 1984.Google Scholar
  9. 9.
    Ohashi Y, Kita S, Murakami T: Microcirculation of the rat small intestine as studied by the injection replica scanning electron microscope method. Arch Histol Jpn 39: 271–282, 1976.PubMedGoogle Scholar
  10. 10.
    Kobayashi A, Takeda S, Hattori S, Kohli Y, Hattori T, Kawai K: Vascular architecture of human gastric mucosa. Jpn J Gastroenterol 73: 169–178, 1976 (in Japanese).Google Scholar
  11. 11.
    Takao, M: Microcirculation of gastric ulcer. A scanning electron microscopic study of vascular casts. Okayama Igakkai Z 90: 915–948, 1978 (in Japanese).Google Scholar
  12. 12.
    Nopanitaya W, Aghajanian JG, Gray LD: An improved plastic mixture for corrosion casting of the gastrointestinal microvascular system. In: Scanning Electron Microscopy/ 1979/111. RP Becker, O Johari (eds), Chicago: AMF O’Hare, SEM Inc, p 751–755, 1979.Google Scholar
  13. 13.
    Tamura A: Scanning electron microscopic studies on lymphatic casts of the rat stomach and the rabbit small intestine. Okayama Igakkai Z 91: 1075–1099, 1979 (in Japanese).Google Scholar
  14. 14.
    Gannon BJ, Rogers PAW, O’Brien PE: Two capillary plexuses in human intestinal villi. Micron 11: 447–448, 1980.Google Scholar
  15. 15.
    Bhalla DK, Murakami T, Owen RL: Microcirculation of intestinal lymphoid follicles in rat Peyer’s patches. Castroenterol 81: 481–491, 1981.Google Scholar
  16. 16.
    Gannon BJ, Gore RW, Rogers PAW: Is there an anatomical basis for a vascular countercurrent mechanism in rabbit and human intestinal villi? Biomed Res 2 (suppl): 235–241, 1981.Google Scholar
  17. 17.
    Gannon B, Browning J, O’Brien P: The microvascular architecture of the glandular mucosa of rat stomach. J Anat 135: 667–683, 1982.PubMedGoogle Scholar
  18. 18.
    Métry JM, Neff M, Knoblauch M: The microcirculatory system of the intestinal mucosa of the rat. An injection cast and scanning electron microscopy study. Scand J Gastroenterol 17 (suppl 71): 159–162, 1982.Google Scholar
  19. 19.
    Bockman DE: Functional histology of appendix. Arch Histol Jpn 46: 271–292, 1983.PubMedCrossRefGoogle Scholar
  20. 20.
    Ohtani O, Kikuta A, Ohtsuka A, Taguchi T, Murakami T: Microvasculature as studied by the microvascular corrosion casting/scanning electron microscope method. I. Endocrine and digestive system. Arch Histol Jpn 46: 1–42, 1983.CrossRefGoogle Scholar
  21. 21.
    Yamaguchi K, Schoefl GI: Blood vessels of the Peyer’s patch in the mouse: I. Topographic studies. Anat Rec 206: 391–401. 1983.PubMedCrossRefGoogle Scholar
  22. 22.
    Gannon B, Browning J, O’Brien P, Rogers P: Mucosal microvascular architecture of the fundus and body of human stomach. Gastroenterol 86: 866–875, 1984.Google Scholar
  23. 23.
    Ohtsuka A, Ohtani O: The microvascular architecture of the rabbit stomach corpus in vascular corrosion casts. In: Scanning Electron Microscopy /1984/IV. O Johari (ed), Chicago: AMF O’Hare, SEM Inc, p 1951–1956, 1984.Google Scholar
  24. 24.
    Ohtani O, Ohtsuka A: Three-dimensional organization of lymphatics and their relationship to blood vessels in rabbit small intestine. A scanning electron microscopic study of corrosion casts. Arch Histol Jpn 48: 255–268, 1985.PubMedCrossRefGoogle Scholar
  25. 25.
    Murakami T, Itoshima T, Hitomi K, Ohtsuka A, Jones AL: A monomeric methyl and hydroxypropyl methacrylate injection medium and its utility in casting blood capillaries and bile canaliculi for scanning electron microscopy. Arch Histol Jpn 47: 223–237, 1984.PubMedCrossRefGoogle Scholar
  26. 26.
    Barlow TE, Bentley FH, Walder DN: Arteries, veins, and arteriovenous anastomoses in the human stomach. Surg Gynecol Obstet 93: 657–671, 1951.PubMedGoogle Scholar
  27. 27.
    Oka S: Studies on the microcirculation of gastrointestinal mucosa. Saishin Igaku 25: 1705–1713, 1970 (in Japanese).PubMedGoogle Scholar
  28. 28.
    Hase T, Moss BJ: Microvascular changes of gastric mucosa in the development of stress ulcer in rats. Gastroenterology 65: 224–234, 1973.PubMedGoogle Scholar
  29. 29.
    Guth PH, Rosenberg A: In vivo microscopy of the gastric microcirculation. Am J Digest Dis 17: 391–398, 1972.PubMedCrossRefGoogle Scholar
  30. 30.
    Piasecki C: Blood supply to the human gastroduodenal mucosa with special reference to the ulcer-bearing areas. J Anat 118: 295–335, 1974.PubMedGoogle Scholar
  31. 31.
    Piasecki C: Observations on the submucous plexus and mucosal arteries of the dog’s stomach and first part of the duodenum. J Anat 119: 133–148, 1975.PubMedGoogle Scholar
  32. 32.
    Fujita H, Fujita T: Textbook of histology, part 2, 2nd ed. Tokyo: Igaku-Shoin, 1984 (in Japanese).Google Scholar
  33. 33.
    Johansson C, Aly A: Stimulation of gastric mucus output by somatostatin in man. Eur J Clin Invest 12: 37–39, 1982.PubMedCrossRefGoogle Scholar
  34. 34.
    Kusumoto Y, Iwanaga T, Ito S, Fujita T: Juxtaposition of somatostatin cell and parietal cell in the dog stomach. Arch Histol Jpn 42: 459–465, 1979.PubMedGoogle Scholar
  35. 35.
    Browning J, Gannon B: The microvascular architecture of rat proximal duodenum, with particular reference to Brunner’s glands. Biomed Res 5: 245–258, 1984.Google Scholar
  36. 36.
    Mall JP: Die Blut- und Lymphwege im Dfinndarm des hundes. Abh Mat-Phys I Konig Sachs Gesell Wiss 14: 153–189, 1887.Google Scholar
  37. 37.
    Spanner R: Neue Befunde über die Blutwege der Darmwand und ihre funktionelle Bedeutung. Morphol Jahrb 69: 394–454, 1932.Google Scholar
  38. 38.
    Jacobson LF, Noer RJ: The vascular pattern of the intestinal villi in various laboratory animals and man. Anat Rec 114: 85–101, 1952.PubMedCrossRefGoogle Scholar
  39. 39.
    Nylander G, Olerud S: The vascular pattern of an isolated jejunal loop. A microangiographic study in the rat. Acta Chir Scand 121: 39–46, 1961.PubMedGoogle Scholar
  40. 40.
    Mohiuddin A: Blood and lymph vessels in the jejunal villi of the white rat. Anat Rec 156: 83–90, 1966.PubMedCrossRefGoogle Scholar
  41. 41.
    Reynolds DG, Brim J, Sheehy TW: The vascular architecture of the small intestinal mucosa of the monkey (Macaca mulatta). Anal Rec 159: 211–218, 1967.CrossRefGoogle Scholar
  42. 42.
    Miller DS, Rahman MA, Tanner R, Mathan VI, Baker SJ: The vascular architecture of the different forms of small intestinal villi in the rat (Rattus norvegicus). Scand J Gastroenterol 4: 477–482, 1969.PubMedCrossRefGoogle Scholar
  43. 43.
    Reynolds DG, Swan KG: Intestinal microvascular architecture in endotoxic shock. Gastroenterol 63: 601–610, 1972.Google Scholar
  44. 44.
    Bellamy JEC, Latshaw WK, Nielsen NO: The vascular architecture of the porcine small intestine. Can J Comp Med 37: 56–62, 1973.PubMedGoogle Scholar
  45. 45.
    Svanvik J: Mucosal blood circulation and its influence on passive absorption in the small intestine. An experimental study in the cat. Acta Physiol Scand (suppl) 385: 1–44, 1973.Google Scholar
  46. 46.
    Winne D: The vasculature of the jejunal villus. Excerpta Medica International Congress Series 391: 56–57, 1973.Google Scholar
  47. 47.
    Szyika AS: Systématisation de l’angioarchitectonie du jéjunum et de l’iléon chez l’homme adulte. Arch Anat Histol Embryol Norm Exp 59: 79–142, 1976.Google Scholar
  48. 48.
    Wolfram-Gabel R: Systématisation de l’angioarchitectonic du jéjunum et de l’iléon chez l’homme en période périnatale. Arch Anat Histol Embryol Norm Exp 63: 39–142, 1980.Google Scholar
  49. 49.
    Wolfram-Gabel R, Maillot Cl: La vascularisation des tuniques sous-muqueuse et muqueuse des première et deuxième portions du duodenum chez l’homme adulte. Arch Anat Histol Embryol Norm Exp 64: 129–171, 1981.Google Scholar
  50. 50.
    Eriksson B: Microangiographic pattern in the small intestine of the cat after irradiaton. Scand J Gastroenterol 17: 887–895, 1982.PubMedCrossRefGoogle Scholar
  51. 51.
    Dabelow A: Die Blutgefäßversorgung der lymphatischen Organe. Verhand Anat Gesel 46 Versam (Anat Anz 87 (Erg)): 179–224, 1939.Google Scholar
  52. 52.
    Abe K, Ito T: A qualitative and quantitative morphologic study of Peyer’s patches of the mouse. Arch Histot Jpn 40: 407–420, 1977.Google Scholar
  53. 53.
    Blau JN: A comparative study of the microcirculation in the guinea pig thymus, lymph nodes and Peyer’s patches. Clin Exp Immunot 27: 340–347, 1977.Google Scholar
  54. 54.
    Gowans JL, Knight EJ: The route of recirculation of lymphocytes in the rat. Proc R Soc Lond (Biol) 159: 257–282, 1964.PubMedCrossRefGoogle Scholar
  55. 55.
    Marchesi VT, Gowans JL: The migration of lymphocytes through the endothelium of venules in lymph nodes: An electron microscope study. Proc R Soc Lond (Biol) 159: 283–290, 1964.PubMedCrossRefGoogle Scholar
  56. 56.
    Browning J, Gannon B: Mucosal microvascular organization of the rat colon. Acta Anat 126: 73–77, 1986.PubMedCrossRefGoogle Scholar
  57. 57.
    Wolfram-Gabel R, Maillot CI, Koritke JG: La vascularisation des tuniques sous-muqueuse et muqueuse du colon chez l’homme. Arch Anat Histol Embryol Norm Exp 66: 67–98, 1983.Google Scholar
  58. 58.
    Owen RL, Nemanic P: Antigen processing structures of the mammalian intestinal tract: An SEM study of lymphoepithelial organs. In: Scanning Electron Microscopy/1978/11 RP Becker, O Johari (eds). Chicago: AMF O’Hare. SEM Inc, p 367–378, 1978.Google Scholar
  59. 59.
    Shimizu S: Beiträge zur Anatomy des Lymphgefässsystems der Wirbeltiere und des Menschen (Japaner). Nr. 8. Darmzotten und ihre Gefässe, insbesondere die Chylusgefässe der Säugetiere und des Menschen. Okajimas Folia Anat Jpn 10: 193–227, 1932.Google Scholar
  60. 60.
    Ohtani O: Three-dimensional organization of lymphatics and its relationship to blood vessels in rat small intestine. Cell Tiss Res 248: 365–374, 1987CrossRefGoogle Scholar
  61. 61.
    Ohtani O, Ohtsuka A, Owen RL: Three-dimensional organization of the lymphatics in the rabbit appendix. A scanning electron and light microscopic study. Gastroenterology 91: 947–955, 1986.PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishing, Boston 1988

Authors and Affiliations

  • Aiji Ohtsuka
    • 1
  • Osamu Ohtani
    • 1
  • Takuro Murakami
    • 1
  1. 1.Department of AnatomyOkayama University Medical SchoolOkayamaJapan

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