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NA/K Pump Function in Cultured Embryonic Chick Heart Cells

  • Chapter
Heart Function and Metabolism

Part of the book series: Developments in Cardiovascular Medicine ((DICM,volume 66))

Abstract

The goal of research in our laboratory is to characterize the mechanisms responsible for ion transport across the cardiac sarcolemma using a multidisciplinary approach that combines biochemical and biophysical techniques. Cultured chick embryo heart cells are an ideal preparation for these studies because cells can be grown in one of several physiologically stable configurations ideally suited for a particular experimental technique (1): confluent mass cultures for biochemical measurements; small (~ 100 μm diameter) aggregates for voltage-clamp analysis; polystrands for isotope flux and ion-selective microelectrodes (ISME). In addition, cultured myocyte preparations are free from the physical (diffusional barriers) and biological (altered metabolic state) limitations associated with dissected cardiac muscle preparations and isolated heart cells, respectively.

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Author information

Authors and Affiliations

  1. Department of Physiology, Duke University Medical Center, Durham, North Carolina, 27710, USA

    L. Anderson Lobaugh, S. Liu & M. Lieberman

Authors
  1. L. Anderson Lobaugh
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  2. S. Liu
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  3. M. Lieberman
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Editor information

Editors and Affiliations

  1. Youville Research Institute, St. Boniface General Hospital, Winnipeg, Canada

    Naranjan S. Dhalla , Grant N. Pierce  & Robert E. Beamish ,  & 

  2. Department of Physiology, Unniversity of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla , Grant N. Pierce  & Robert E. Beamish ,  & 

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© 1987 Martinus Nijhoff Publishing, Boston

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Lobaugh, L.A., Liu, S., Lieberman, M. (1987). NA/K Pump Function in Cultured Embryonic Chick Heart Cells. In: Dhalla, N.S., Pierce, G.N., Beamish, R.E. (eds) Heart Function and Metabolism. Developments in Cardiovascular Medicine, vol 66. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-2053-1_11

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  • DOI: https://doi.org/10.1007/978-1-4613-2053-1_11

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4612-9220-3

  • Online ISBN: 978-1-4613-2053-1

  • eBook Packages: Springer Book Archive

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