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The pH-Dependence of the Reductional Levels of Cytochrome c and Cytochrome a During Turnover of Cytochrome c Oxidase

  • Per-Eric Thörnström
  • Brigitta Maison-Peteri
  • Peter Brzezinski
  • Lars Arvidsson
  • Per-Olof Fredriksson
  • Bo G. Malmström

Abstract

The four redox centers of cytochrome oxidase (the cytochromes a and a3 and the coppers CuA. and CuB) are since long known to be involved in electron transport through the enzyme, leading to the reduction of dioxygen. Now it is also known that the enzyme is a proton pump. The energy needed for pumping protons is derived from electron transport, so that the requirements for a redox-linked proton pump must be fullfilled. Namely: 1. Two different conformations must account for an alternating access of a proton-translocating group to the two different sides of the membrane. 2. A redox center must be reduced in one conformation and reoxidized in the other conformation. This also means that there is an obligatory coupling between electron transfer and proton pumping (1, 2),

Keywords

Electron Transfer Electron Transport Proton Pump Cytochrome Oxidase Reductional Level 
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References

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    M. Fabian, P.-E. Thornstrom, P. Brzezinski, and B.G. Malmström (1987) FEBS Lett. 213, 396–400.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Per-Eric Thörnström
    • 1
    • 2
  • Brigitta Maison-Peteri
    • 1
    • 2
  • Peter Brzezinski
    • 1
    • 2
  • Lars Arvidsson
    • 1
    • 2
  • Per-Olof Fredriksson
    • 1
    • 2
  • Bo G. Malmström
    • 1
    • 2
  1. 1.Department of Biochemistry and BiophysicsChalmers University of TechnologySweden
  2. 2.University of GöteborgGöteborgSweden

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