Cytochrome C Oxidase: Past, Present and Future

  • Michele Müller
  • Nestor Labonia
  • Beatrice Schläpfer
  • Angelo Azzi

Abstract

In the present report the last ten years of scientific research in our laboratory will be reviewed. A particular accent will be given to the data being presently elaborated and to the future lines which can be already forseen. The work with cytochrome c oxidase has experienced the same evolution as the general field of bioenergetics: Studies centered largely on function have developed into more structural research to end up in the beginning of the molecular biology of such a complex enzyme.

Keywords

Permeability Lithium Cystein Respiration Sedimentation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Azzi, K. Bill, and C. Broger, Affinity chromatography purification of cytochrome c binding enzymes, Proc. Natl. Acad. Sci USA 79: 2447 (1982).CrossRefGoogle Scholar
  2. 2.
    K. Bill and A. Azzi, Interaction of reduced and oxidized cytochrome c with the mitochondrial cytochrome c oxidase and be-j-complex, Biochem. Biophys. Res. Commun. 120: 124 (1984).CrossRefGoogle Scholar
  3. 3.
    K. Bill, R.P. Casey, C. Broger, and A. Azzi, Affinity chromatography purification of cytochrome c oxidase: Use of a yeast cytochrome c-thiol-sepharose 4B column, FEBS Lett. 120: 248 (1980).CrossRefGoogle Scholar
  4. 4.
    K. Bill, C. Broger, and A. Azzi, Affinity chromatography purification of cytochrome c oxidase and b-c-j complex from beef heart mitochondria. Use of thiol-Sepharose-bound Saccharomyces cerevisiae cytochrome c, Biochim. Biophys. Acta 679: 28 (1982).CrossRefGoogle Scholar
  5. 5.
    A. Azzi and R.B. Gennis, Purification of the aa3-type cytochrome-c oxidase from Rhodopseudomonas sphaeroides, Methods Enzymol. 126: 138 (1986).CrossRefGoogle Scholar
  6. 6.
    W. deVrij, B. Poolman, W.N. Konings, and A. Azzi, Purification, enzymatic properties, and reconstitution of cytochrome-c oxidase from Bacillus subtilis, Methods Enzymol. 126: 159 (1986).CrossRefGoogle Scholar
  7. 7.
    R.B. Gennis, R.P. Casey, A. Azzi, and B. Ludwig, B. Purification and characterization of the cytochrome c oxidase from Rhodopseudomonas sphaeroides, Eur. J. Biochem. 125: 189 (1982).CrossRefGoogle Scholar
  8. 8.
    W. deVrij, A. Azzi, and W.N. Konings, Structural and functional properties of cytochrome c oxidase from Bacillus subtilis W23, Eur. J. Biochem. 131: 97 (1983).CrossRefGoogle Scholar
  9. 9.
    M.J. Corbley and A. Azzi, Resolution of bovine heart cytochrome c oxidase into smaller complexes by controlled subunit denaturation, Eur. J. Biochem. 139: 535 (1984).CrossRefGoogle Scholar
  10. 10.
    R. Bisson, H. Gutweniger, and A. Azzi, Photoaffinity labeling of yeast cytochrome oxidase with arylazido cytochrome c derivatives, FEBS Lett. 92: 219 (1978).CrossRefGoogle Scholar
  11. 11.
    R. Bisson, A. Azzi, H. Gutweniger, R. Colonna, C. Montecucco, and A. Zanotti, Interaction of cytochrome c with cytochrome c oxidase. Photoaffinity labeling of beef heart cytochrome c oxidase with arylazido-cytochrome c, J. Biol. Chem. 253: 1874 (1978).Google Scholar
  12. 12.
    R. Bisson, H. Gutweniger, C. Montecucco, R. Colonna, A. Zanotti, and A. Azzi, Covalent binding of arylazido derivatives of cytochrome c to cytochrome oxidase, FEBS Lett. 81: 147 (1977).CrossRefGoogle Scholar
  13. 13.
    R. Bisson, C. Montecucco, H. Gutweniger, and A. Azzi, Hydrophobic labelling of bovine heart cytochrome c oxidase with an azido- phosphatidylcholine, Biochem. Soc. Trans. 7: 156 (1979).Google Scholar
  14. 14.
    R. Bisson, C. Montecucco, H. Gutweniger, and A. Azzi, Cytochrome c oxidase subunits in contact wit phospholipids. Hydrophobic photo- labeling with azidophospholipids, J. Biol. Chem. 254: 9962 (1979).Google Scholar
  15. 15.
    R. Bolli, K.A. Nałcz, and A. Azzi, The aggregation state of bovine heart cytochrome c oxidase and its kinetics in monomeric and dimeric form, Arch. Biochem. Biophys. 240: 102 (1985).CrossRefGoogle Scholar
  16. 16.
    K.A. Nałecz, R. Bolli, B. Ludwig, and A. Azzi, The role of subunit III in bovine cytochrome c oxidase. Comparison between native, subunit Ill-depleted and Paracoccus denitrificans enzymes, Biochim. Biophys. Acta 808: 259 (1985).CrossRefGoogle Scholar
  17. 17.
    R. Bolli, K.A. Nałcz, and A. Azzi, The interconversion between monomeric and dimeric bovine heart cytochrome c oxidase, Biochimie 67: 119 (1985).CrossRefGoogle Scholar
  18. 18.
    K.A. Nałcz, R. Bolli, and A. Azzi, Preparation of monomeric cytochrome c oxidase: its kinetics differ from those of the dimeric enzyme, Biochem. Biophys. Res. Commun. 114: 822 (1983).CrossRefGoogle Scholar
  19. 19.
    R. Bolli, K.A. Nałcz, and A. Azzi, Cytochrome c oxidase from Paracoccus denitrificans in Triton X-100: Aggregation state and kinetics, J. Bioenerg. Biomembr. 18: 277 (1986).CrossRefGoogle Scholar
  20. 20.
    K.A. Nałcz, R. Bolli, and A. Azzi, Techniques for the study of bovine cytochrome-c oxidase monomer-dimer association, Methods Enzymol. 126: 45 (1986).CrossRefGoogle Scholar
  21. 21.
    R.P. Casey, P.S. O’Shea, J.B. Chappell, and A. Azzi, A quantitative characterisation of H+ translocation by cytochrome c oxidase vesicles, Biochim. Biophys. Acta 765: 30 (1984).CrossRefGoogle Scholar
  22. 22.
    A. Azzi, Cytochrome c oxidase. Towards a clarification of its structure, interactions and mechanism, Biochim. Biophys. Acta 594: 231 (1980).Google Scholar
  23. 23.
    M. Mueller, M. Thelen, P.S. O’Shea, and A. Azzi, Functional reconstitution of proton-pumping cytochrome-c oxidase in phospholipid vesicles, Methods Enzymol. 126: 78 (1986).CrossRefGoogle Scholar
  24. 24.
    R.P. Casey, C. Broger, M. Thelen, and A. Azzi, Studies on the molecular basis of H+ translocation by cytochrome c oxidase, J. Bioenerg. Biomembr. 13: 219 (1981).CrossRefGoogle Scholar
  25. 25.
    A. Azzi, Mitochondria: the utilization of oxygen for cell life, Experientia 49: 901 (1984).CrossRefGoogle Scholar
  26. 26.
    R.P. Casey and A. Azzi, An evaluation of the evidence for H+ pumping by reconstituted cytochrome c oxidase in the light of recent criticism, FEBS Lett. 154: 237 (1983).CrossRefGoogle Scholar
  27. 27.
    M. Thelen, P.S. O’Shea, G. Petrone, and A. Azzi, Proton translocation by a native and subunit Ill-depleted cytochrome c oxidase reconstituted into phospholipid vesicles. Use of fluorescein-phosphatidylethanolamine as an intravesicular pH indicator, J. Biol. Chem. 260: 3626 (1985).Google Scholar
  28. 28.
    A. Azzi, M. Mueller, P.S. O’Shea, and M. Thelen, Molecular properties of reconstituted cytochrome c oxidase: New evidence supports vectorial proton translocation, J. Inorg. Biochem. 23: 341 (1985).CrossRefGoogle Scholar
  29. 29.
    M. Thelen, P.S. O’Shea, and A. Azzi, New insights on the cytochrome c oxidase proton pump, Biochem. J. 227: 63 (1985).Google Scholar
  30. 30.
    R.P. Casey, M. Thelen, and A. Azzi, Dicyclohexylcarbodiimide binds specifically and covalently to cytochrome c oxidase while inhibiting its H+-translocating activity, J. Biol. Chem. 255: 3994 (1980).Google Scholar
  31. 31.
    R.P. Casey, C. Broger, and A. Azzi, Structural studies on the cytochrome c oxidase proton pump using a spin-label probe, Biochim. Biophys. Acta 638: 86 (1981).CrossRefGoogle Scholar
  32. 32.
    A. Azzi, R.P. Casey, and M.J. Nałcz, The effect of N,Nf-Dicyclo-hexylcarbodiimide on enzymes of bioenergetic relevance, Biochim. Biophys. Acta 768: 209 (1984).Google Scholar
  33. 33.
    M.J. Nałcz, R.P. Casey, and A. Azzi, Use of N,Nf-dicyclohexylcarbodiimide to study membrane-bound enzymes, Methods Enzymol. 125: 86 (1986).Google Scholar
  34. 34.
    R.P. Casey, B.H. Ariano, and A. Azzi, Studies on the transmembrane orientation of cytochrome c oxidase in phospholipid vesicles, Eur. J. Biochem. 122: 313 (1982).CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Michele Müller
    • 1
  • Nestor Labonia
    • 1
  • Beatrice Schläpfer
    • 1
  • Angelo Azzi
    • 1
  1. 1.Institut für Biochemie und MolekularbiologieUniversität BernBernSwitzerland

Personalised recommendations