Summary
Import of precursors of cytochromes b2 and c1 into mitochondria requires a mitochondrial membrane potential. We show here that in addition ΔΨ, nucleoside triphosphates (NTPs) are necessary for protein translocation. At low concentrations of NTPs, intermediate-sized cytochrome b2 was accumulated spanning the outer and inner membranes at contact sites. For complete translocation into mitochondria, higher concentrations of NTPs were necessary. We conclude that different levels of NTPs are required for distinct steps in the import pathway.
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© 1987 Plenum Press, New York
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Hartl, FU., Ostermann, J., Pfanner, N., Tropschug, M., Guiard, B., Neupert, W. (1987). Import of Cytochromes b2 and c1 into Mitochondria is Dependent on Both Membrane Potential and Nucleoside Triphosphates. In: Papa, S., Chance, B., Ernster, L. (eds) Cytochrome Systems. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1941-2_26
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DOI: https://doi.org/10.1007/978-1-4613-1941-2_26
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9078-0
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