Antibody Guided Diagnosis and Therapy of Patients with Breast Cancer

  • Haralambos P. Kalofonos
  • Agemenmon A. Epenetos


Tumour associated monoclonal antibodies HHFG1 and HMFG2, labelled with iodine-123, and F(ab’)2 of HMFG1, labelled with indium-Ill, were used to detect primary and metastatic breast cancer, by external body scintigraphy, in 13 patients with measurable disease. Tumours became visible in the majority (9 out of 13) of patients. We detected both primary breast cancer and bony and soft tissue metastases. The presence of antibody in the tumours was confirmed by autoradiography and immunoperoxidase staining of surgically removed tissues. Ill-In labelled F(ab’)2 fragments were superior to whole antibody. We imaged successfully the disease in 5 out of 7 patients, injected with radiolabeled F(ab’)2 °f HMFG1. 2 patients with negative antibody scans had received prior chemotherapy and were in clinical remission.

Tumour associated monoclonal antibodies HHFG1, HMFG2 and AUA1 radiolabeled with iodine-131, were given intracavitary (intrapleurally and intrapericardially) to patients with malignant serous effusions secondary to breast and other epithelial cancers. Ten out of thirteen effusions (3 pericardial and 7 pleural) 1n 11 patients, responded completely with no fluid reaccumulation, between 3 and 18 months. No toxicity was encountered.

We conclude that this new method of treatment is nontoxic and effective, resulting in improved quality of life and in some cases, prolongation of survival of patients with breast or other epithelial origin neoplasms.


Breast Cancer Pericardial Effusion Malignant Pleural Effusion Malignant Effusion Medical Internal Radiation Dose 
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Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Haralambos P. Kalofonos
    • 1
  • Agemenmon A. Epenetos
    • 1
  1. 1.Imperial Cancer Research Fund Oncology Group, Department of Clinical OncologyRoyal Postgraduate Medical School, Hammersmith Hospital and Imperial Cancer Research FundLondonUK

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