Lectins and Lectin Receptors on β-Lactam Resistant and Susceptible PseudomonasAeruginosa Strains: Significance for Attachment and Intracellular Killing by Human PMN

  • G. Stübner
Chapter

Abstract

Polymorphonuclear granulocytes (PMN) constantly come in contact with particles in a large number, but they attack and ingest only a few, because their plasma membrane bears structures that recognize specific molecules on the surface of the particles, and therefore, they are able to discriminate between ordered well known and disordered foreign structures (8). In recent years, evidence has been accumulated suggesting that sugar binding proteins, the lectins, being present on bacterial surfaces may serve as attachment moieties of certain pathogens to sugar residues on a variety of animal cells (3,6,10,13,14,17,18).

Keywords

Sugar Carbohydrate EDTA Glycine Sedimentation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Z. Bar-Shavit, J. Ofek and R. Goldman, Mannose residues on phagocytes as receptors for the attachment of Escherichia coli and Salmonella typhi, Biochem. Biophys. Res. Commun. 78:455 (1977).PubMedCrossRefGoogle Scholar
  2. 2.
    A. Bøyum, Separation of leukocytes from blood and bone marrow, Scand. J. clin. Invest. 21(Suppl. 97) (1968).Google Scholar
  3. 3.
    W. A. Collier and J. C. de Miranda, Bakterienhamagglutination I. Versuche mit einem hamagglutinierenden Stamm von E. coli, Antonie von Leeuwenhoek, J. Microbiol. Serol. 21:133 (1955).Google Scholar
  4. 4.
    M. C. Connelly, D. C. Stein, F. E. Young, S. A. More, and P. Z. Allen, Interaction with lectins and differential wheat germ agglutinin binding of pyocin 103 sensitive and resistant Neisseria gonorrhoeae, J. Bacteriol. 148:796 (1981).PubMedGoogle Scholar
  5. 5.
    J. W. Costerton, Pseudomonas aeruginosa in nature and disease, in: “Pseudomonas aeruginosa”. C. D. Sabath, ed., Huber, Bern. (1979).Google Scholar
  6. 6.
    R. Goldman and Z. Bar-Shavit, Phagocytosis — modes of particle recognition and stimulation by natural peptides, in: “Phagocytosis-Past and Future,” L. Karnovsky and L. Bolis, eds., Academic Press, New York, London (1982).Google Scholar
  7. 7.
    I. J. Goldstein and C. E. Hayes, The lectins: carbohydrate-binding proteins of plants and animals, in: “Advances in Carbohydrate Chemistry and Biochemistry,” R. S. Tipson and D. Horton, eds., Academic Press, New York, San Francisco, London (1978).Google Scholar
  8. 8.
    F. M. Griffin, Mononuclear cell phagocytic mechanisms and host defense, in: “Advances in Host Defense Mechanisms,” J. I. Gallin and A. S. Fauci, eds., Raven Press, New York (1982).Google Scholar
  9. 9.
    D. A. Hart, Lectins in biological systems: applications to microbiology, Am. J. Clin. Nutr. 33:2416 (1980).PubMedGoogle Scholar
  10. 10.
    K. Jann, B. Jann, G. Schmidt and K. Vosbeck, Adhesion of Escherichia coli mammalian and yeast cells: role of piliation and hydrophobicity, Infect. Immun. 32:484 (1981).PubMedGoogle Scholar
  11. 11.
    M. A. Kenward, R. W. Brown, S. R. Hesslewood and C. Dillon, Influence of R - plasmid RP1 of Pseudomonas aeruginosa on cell wall composition, drug resistance and sensitivity to cold shock, Antimicrob. Agents Chemother. 13:446 (1978).PubMedGoogle Scholar
  12. 12.
    H. Lis and N. Sharon, Lectins: Their chemistry and application in immunology, in: “The Antigens,” M. Sela, ed., Academic Press, New York (1977).Google Scholar
  13. 13.
    I. Ofek, D. Mirelman and N. Sharon, Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors, Nature (London) 265:623 (1977).CrossRefGoogle Scholar
  14. 14.
    G. Rottini, F. Cian, M. R. Sorenzo, R. Albrigo and P. Patriarca, Evidence for the involvement of human polymorphonuclear leucocyte mannose-like receptors in the phagocytosis of Escherichia coli, FEBS Letters 105:307 (1979).PubMedCrossRefGoogle Scholar
  15. 15.
    G. Stubner and A. Dalhoff, Effect of subinhibitory β-lactam concentrations on intracellular killing of Ps.aeruginosa by human polymorphonuclear granulocytes, 13th International Congress for Chemotherapy, Vienna, Abstract SE 3.2./1-4 (1983).Google Scholar
  16. 16.
    G. Stubner and R. Marre, Use of lectins to characterize surface alterations of β-lactam resistant mutants of Pseudomonas aeruginosa, Symposium: The Influence of Antibiotics on the Host-Parasite Relationship, Munich (1985).Google Scholar
  17. 17.
    G. Uhlenbruck, R. Gross, O. Koch et al., Die Bedeutung von Lektinen fur den Adhasionsmechanismus von Bakterien, Deutsches Arzteblatt 80:27 (1983).Google Scholar
  18. 18.
    D. M. Weir and H. Ogmundsdottir, Non-specific recognition mechanisms by mononuclear phagocytes, Clin. Exp. Immunol. 30:323 (1977).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • G. Stübner
    • 1
  1. 1.Institut für Bakteriologie und HygieneKrankenhaus NordstadtHannoverGermany

Personalised recommendations