The Proteins in Avian Eggs: A Test of Protein Separation Methods Based On HPLC
The vitelline membrane. A CI8 column was suitable for separating six proteins from the outer layer of this membrane. Three of these were isolated and partial N-terminal sequences determined on a gas-phase sequencer. One proved to be egg lysozyme; another VMOI (vitelline membrane outer I) and the third has been named VMOII.
Egg yolk low density lipoprotein. The apoproteins from this lipoprotein were separated on a phenyl hydrophobic column using a gradient of acidic urea. The results were similar to those obtained with phenyl Sepharose.
Egg yolk livetins. A column of DEAE — 5 PW was used to separate the main proteins using an ionic-strength gradient. A comparison was made with blood serum.
Albumen (i.e. egg white). The same column was used with the same eluant, which separated the main egg-white proteins.
For each of these analyses the samples required a minimum of preparation.
KeywordsHPLC Urea Acetonitrile Sodium Chloride Phenyl
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