Abstract
Human T-lymphotropic virus type III/lymphadenopathy virus (HTLV- III/LAV) is the etiologic agent of acquired immunodeficiency syndrome (AIDS),1-2 although cofactors may play a significant role in disease expression.5 Changes in social behavior may reduce the rate of AIDS transmission, but complete curtailment of the epidemic seems unlikely without immunological or pharmacological intervention. Much effort has been invested in the direct examination of the virus and its infection processes to elucidate promising strategies of intervention, one of which may be vaccination, Detailed information on HTLV-III/LAV components, especially proteins, should aid the rational design of vaccines by focusing attention on those viral constituents likely to induce protective immunity.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
M. Essex, M. F. McLane, T. H. Lee, L. Falk, C. W. S. Howe, J. I. Mullins, C. Cabradilla, and D. P. Francis, Antibodies to cell membrane antigens associated with human T-cell leukemia virus in patients with AIDS, Science 220: 859 (1983).
F. Barre-Sinoussi, J. C. Chermann, F. Rey, M. T. Nugeyre, S. Chamaret, J. Gruest, C. Dauguet, F. Axler-Bain, F. Vezinet-Brun, C. Rouzioux, W. Rozenboum, and L. Montagnier, Isolation of a T-lymphotropic retrovirus from a patient at risk of acquired immunodeficiency syndrome (AIDS), Science 220: 868 (1983).
R. C. Gallo, S. Z. Salahuddin, M. Popovic, G. M. Shearer, M. Kaplan, B. F. Haynes, T. J. Palker, R. Redfield, J. Oleske, B. Safai, G. White, P. Foster, and P. D. Markham, Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS, Science 224: 500 (1984).
J. A. Levy, A. D. Hoffman, S. M. Kramer, J. A. Landis, J. M. Shimabukuro, and L. S. Oshiro, Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS, Science 225: 840 (1984).
R. J. Albin, AIDS: A noncommunicable cofactor, JAMA 253 (23): 3398 (1985).
H. W. Haverkos and R. Edelman, Female-to-male transmission of AIDS, JAMA 254 (8): 1035 (1985).
I. Toplin and P. Sottong, Large-volume purification of tumor viruses by use of zonal centrifuges, App. Microbiol. 23: 1010 (1972).
J. C. Leavitt, M. A. Phelan, A. H. Leavitt, R. E. Mayner, and F. A. Ennis, Human influenza A virus: Comparative analysis of the structural polypeptides by two-dimensional polyacrylamide gel electrophoresis, Virology 99: 340 (1979).
B. J. Poiesz, F. W. Ruscetti, A. F. Gazdan, P. A. Bunn, J. D. Minna, and R. C. Gallo, Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma, Proc. Natfl. Acad. Sci. USA 77: 7415 (1980).
D. C. Redfield, D. D. Richman, M. N. Oxman, and L. H. Kronenberg, Psoralen inactivation of influenza and herpes simplex viruses and of virus-infected cells, Infect. Immun. 32: 1216 (1981).
M. Popovic, M. G. Sarngadharan, E. Read, and R. C. Gallo, Detection, isolation, and continuous production of cytopathic retroviruses (HTLV-III) from patients with AIDS and Pre-AIDS, Science 224: 497 (1984).
B. R. Murphy, M. A. Phelan, D. L. Nelson, R. Yarchoan, E. L. Tierney, D. A. Ailing, and R. M. Chanock, Hemagglutinin-specific enzyme- linked immunosorbent assay for antibodies to influenza A and B viruses, J. Clin. Micro. 13 (3): 554 (1981).
U. K. Laemmli, Cleavage of structural proteins during assembly of the head of bacteriophage T4, Nature (London) 277: 680 (1970).
H. Towbin, T. Staehelin, and J. Grodon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications, Proc. Nat’l. Acad. Sci. USA 76: 4350 (1979).
J. C. S. Clegg, Glycoprotein detection in nitrocellulose transfers of electrophoretically separated protein mixtures using Concanavalin A and peroxidase: Application to Arenavirus and Flavivirus proteins, Anal. Biochem. 127: 389 (1982).
M. G. Sarngadharan, L. Bruch, M. Popovic, and R. C. Gallo, Immunological properties of the Gag protein p24 of the acquired immunodeficiency syndrome retrovirus (human T-cell leukemia virus type-Ill), Proc. Nat’l. Acad. Sci. USA 82: 3481 (1985).
C. V. Hanson, Inactivation of viruses for use as vaccines and immunodiagnostic reagents, in: “Medical Virology 2,” L. M. de la Maza and E. M. Peterson, eds., Elsevier Science Publishing Co., Inc., New York (1983).
L. Montagnier, F. Clavel, B. Krust, S. Chamaret, F. Rey, F. Barre-Sinoussi, and J. C. Chermann, Identification and antigenicity of the major envelope glycoprotein of lymphadenopathy-associated virus, Virology 144: 283 (1985).
V. S. Kalyanaraman, C. D. Cabradilla, J. P. Getchell, R. Narayanan, E. H. Braff, J.-C. Chermann, F. Barre-Sinoussi, L. Montagnier, T. J. Spira, J. Kaplan, D. Fishbein, H. W. Jaffe, J. W. Curran, and D. P. Francis, Antibodies to the core protein of lymphadenopathy- associated virus (LAV) in patients with AIDS, Science 225: 321 (1984).
J. L. Saborio, F. Diaz-Barriga, G. Duran, V. Tsutsumi, and E. Palmer, Purification and characterization of GP-55, a protein associated with actin-based cytoplasmic gels derived from brain tissue, J. Biol. Chem. 260: 8627 (1985).
W. G. Robey, B. Safai, S. Oroszlan, L. O. Arthur, M. A. Gonda, R. C. Gallo, and P. J. Fischinger, Characterization of envelope and core structural gene products of HTLV-III with sera from AIDS patients, Science 228: 593 (1985).
J. Schneider, H. Bayer, U. Bienzle, and G. Hunsmann, A glycopoly-peptide (gp 100) is the main antigen detected by HTLV-III antisera, Med. Microbiol. Immunol. 174: 35 (1985).
J. S. Allan, J. E. Coligan, F. Barin, M. F. McLane, J. G. Sodroski, C. A. Rosen, W. A, Haseltine, T. H. Lee, and M. Essex, Major glycoprotein antigens that induce antibodies in AIDS patients are encoded by HTLV-III, Science 228: 1091 (1985).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Phelan, M. et al. (1987). HTLV-III/LAV particle-associated proteins: I. Virus purification, inactivation and basic characterization. In: L’Italien, J.J. (eds) Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1787-6_75
Download citation
DOI: https://doi.org/10.1007/978-1-4613-1787-6_75
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4612-9001-8
Online ISBN: 978-1-4613-1787-6
eBook Packages: Springer Book Archive