Proteins pp 455-460 | Cite as

Computer Analysis of Protein Sequencing Data

  • Norman Froelich
  • Lynn C. Williams
  • John T. Casagrande
  • Minnie McMillan

Abstract

The concept of a Microcbemical facility, in which sophisticated instruments for the analysis and synthesis of genes and proteins of biological significance are centralized in one laboratory and are available to principal investigators on a service basis, serves as a powerful, effective method of interfacing the highly efficient and complex chemistries utilized by these machines with fundamental problems in biology (1). The cornerstone of such a facility is the gas-phase sequenator service which is used by investigators who are cognizant of the fundamental importance and clinical relevance of their proteins, but who are often not familiar with the techniques of protein purification or protein sequencing. In order to assist these investigators in interpretation of their results we have developed a computer program for the analysis of protein-sequencing data. This program plots peak area for each PTH amiho acid for every cycle in the sequencing run. From such plots, one can readily establish the sequences of pure proteins by inspection; in addition, one can; analyse minor species and monitor the technical performance of the sequenator and autosampler, for example. This system is likely to be but a primitive prototype of one in which a protein, after being loaded on the sequenator, will be automatically analyzed and the sequence(s) directly printed out by computer.

Keywords

HPLC Proline Arginine Histidine Leucine 

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References

  1. (1).
    M. Hunkapiller, S. Kent, M. Carutbers, W. Dreyer, J. Firca, C. Giffin, S. Horvatb, T. Hunkapiller, P. Tempst, and L. Hood, Nature 310: 105 (1984).PubMedCrossRefGoogle Scholar
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    R. Hewick, M. Hunkapiller, L. Hood and W. Dreyer, J. Biol. Chem. 256: 7990 (1981).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1987

Authors and Affiliations

  • Norman Froelich
    • 1
  • Lynn C. Williams
    • 1
  • John T. Casagrande
    • 1
  • Minnie McMillan
    • 1
  1. 1.Norris Cancer CenterUSC School of MedicineLos AngelesUSA

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