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Proteins pp 283–298Cite as

Structural Analysis of Proteins of the Nervous System

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Abstract

Proteins and peptides are essential to normal development, growth, and function of the nervous system1. The structural analysis of these proteins and peptides is complicated by the difficulty in obtaining sufficient material for complete, chemical characterization. It is difficult to obtain a homogeneous population of cells to serve as the starting material for protein isolation because the tissues of the nervous system, including brain, spinal cord, peripheral nerves, and sensory systems, are composed of multiple cell types2. Primary cultures of specific populations of cells3, 4 have been useful as models of neural development and function but are limited in their general application. Systems of cultured cells 5, 6, such as embryonic neurons, glia, neuroblastomas, or gliomas, that proliferate in culture also have limited roles as models of normal nervous system function. Therefore, upon purification land characterization of a protein of the nervous system, it is imperative to return to the whole organism to demonstrate the physiological function, anatomical distribution, and pharmacological regulation of the protein. In this way, the structural analysis of proteins of the nervous system is one part of a multidisclplinary approach to neuroscience.

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  1. Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, Maryland, USA

    Daniel R. Marshak

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  1. Molecular Genetics, Inc, Minnetonka, Minnesota, USA

    James J. L’Italien

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© 1987 Plenum Press, New York

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Marshak, D.R. (1987). Structural Analysis of Proteins of the Nervous System. In: L’Italien, J.J. (eds) Proteins. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-1787-6_29

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